John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • M.D., University of Toledo, 1974

Publications

Perfect, JR. "Fungal diagnosis: how do we do it and can we do better?." Curr Med Res Opin 29 Suppl 4 (April 2013): 3-11. (Review)

PMID
23621588
Full Text

Moss, HB, Fowler, WC, Schell, WA, Perfect, JR, and Zedek, DC. "Dematiaceous fungi mimicking a conjunctival melanoma." Ophthal Plast Reconstr Surg 29, no. 2 (March 2013): e65-e66.

PMID
23250331
Full Text

Perfect, JR, and Andes, D. "Antifungal therapy: current concepts and evidence-based management." Curr Med Res Opin (January 22, 2013).

PMID
23336606
Full Text

Smeekens, SP, Ng, A, Kumar, V, Johnson, MD, Plantinga, TS, van Diemen, C, Arts, P, Verwiel, ETP, Gresnigt, MS, Fransen, K, van Sommeren, S, Oosting, M, Cheng, S-C, Joosten, LAB, Hoischen, A, Kullberg, B-J, Scott, WK, Perfect, JR, van der Meer, JWM, Wijmenga, C, Netea, MG, and Xavier, RJ. "Functional genomics identifies type I interferon pathway as central for host defense against Candida albicans." Nat Commun 4 (2013): 1342-.

PMID
23299892
Full Text

Chen, Y-L, Lehman, VN, Averette, AF, Perfect, JR, and Heitman, J. "Posaconazole exhibits in vitro and in vivo synergistic antifungal activity with caspofungin or FK506 against Candida albicans." PLoS One 8, no. 3 (2013): e57672-.

PMID
23472097
Full Text

Liu, T-B, Kim, J-C, Wang, Y, Toffaletti, DL, Eugenin, E, Perfect, JR, Kim, KJ, and Xue, C. "Brain inositol is a novel stimulator for promoting Cryptococcus penetration of the blood-brain barrier." PLoS Pathog 9, no. 4 (2013): e1003247-.

PMID
23592982
Full Text

Boehm, RD, Miller, PR, Schell, WA, Perfect, JR, and Narayan, RJ. "Inkjet printing of amphotericin B onto biodegradable microneedles using piezoelectric inkjet printing." JOM 65, no. 4 (2013): 525-533.

Full Text

Rubach, MP, Pavlisko, EN, and Perfect, JR. "Pericarditis mediated by respiratory syncytial virus in a hematopoietic stem cell transplant patient." Transplant Infectious Disease 15, no. 4 (2013): E152-E156.

Full Text

Perfect, JR. "Iatrogenic fungal meningitis: tragedy repeated." Ann Intern Med 157, no. 11 (December 4, 2012): 825-826.

PMID
23080380
Full Text

Plantinga, TS, Johnson, MD, Scott, WK, Joosten, LAB, van der Meer, JWM, Perfect, JR, Kullberg, BJ, and Netea, MG. "Human genetic susceptibility to Candida infections." Med Mycol 50, no. 8 (November 2012): 785-794. (Review)

PMID
22662758
Full Text

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