John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan at Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., University of Toledo, 1974

Publications

Walton, EM, Cronan, MR, Beerman, RW, and Tobin, DM. "The Macrophage-Specific Promoter mfap4 Allows Live, Long-Term Analysis of Macrophage Behavior during Mycobacterial Infection in Zebrafish." PloS one 10, no. 10 (January 2015): e0138949-.

PMID
26445458
Full Text

Molefi, M, Chofle, AA, Molloy, SF, Kalluvya, S, Changalucha, JM, Cainelli, F, Leeme, T, Lekwape, N, Goldberg, DW, Haverkamp, M, Bisson, GP, Perfect, JR, Letang, E, Fenner, L, Meintjes, G, Burton, R, Makadzange, T, Ndhlovu, CE, Hope, W, Harrison, TS, and Jarvis, JN. "AMBITION-cm: Intermittent high dose AmBisome on a high dose fluconazole backbone for cryptococcal meningitis induction therapy in sub-Saharan Africa: Study protocol for a randomized controlled trial." Trials (2015).

Full Text

Vehreschild, MJGT, Vehreschild, JJ, Marty, FM, Perfect, JR, Ostrosky-Zeichner, L, Rahav, G, Zeiher, B, Lee, M, Maher, R, Lovell, C, Engelhardt, M, and Cornely, OA. "Primary Treatment of Invasive Mucormycosis (IM) with Isavuconazole (VITAL Study) or Amphotericin Formulations (FungiScope (TM)): Case Matched Analysis." December 6, 2014.

Scholars@Duke

Ten Oever, J, Kox, M, van de Veerdonk, FL, Mothapo, KM, Slavcovici, A, Jansen, TL, Tweehuysen, L, Giamarellos-Bourboulis, EJ, Schneeberger, PM, Wever, PC, Stoffels, M, Simon, A, van der Meer, JWM, Johnson, MD, Kullberg, B-J, Pickkers, P, Pachot, A, Joosten, LAB, and Netea, MG. "The discriminative capacity of soluble Toll-like receptor (sTLR)2 and sTLR4 in inflammatory diseases." BMC immunology 15 (November 19, 2014): 55-.

PMID
25406630
Full Text

Chowdhary, A, Perfect, J, and de Hoog, GS. "Black Molds and Melanized Yeasts Pathogenic to Humans." Cold Spring Harbor perspectives in medicine 5, no. 8 (November 10, 2014): a019570-. (Review)

PMID
25384772
Full Text

Köhler, JR, Casadevall, A, and Perfect, J. "The spectrum of fungi that infects humans." Cold Spring Harbor perspectives in medicine 5, no. 1 (November 3, 2014): a019273-. (Review)

PMID
25367975
Full Text

Perfect, JR, Hachem, R, and Wingard, JR. "Update on epidemiology of and preventive strategies for invasive fungal infections in cancer patients." Clinical infectious diseases : an official publication of the Infectious Diseases Society of America 59 Suppl 5 (November 2014): S352-S355. (Review)

PMID
25352630
Full Text

Duarte, RF, López-Jiménez, J, Comely, OA, Laverdiere, M, Helfgott, D, Haider, S, Chandrasekar, P, Langston, A, Perfect, J, Ma, L, Van Lersel, MLPS, Connelly, N, Kartsonis, N, and Waskin, H. "Phase 1b study of new posaconazole tablet for prevention of invasive fungal infections in high-risk patients with neutropenia." Antimicrobial Agents and Chemotherapy 58, no. 10 (October 1, 2014): 5758-5765.

Full Text

Duarte, RF, López-Jiménez, J, Comely, OA, Laverdiere, M, Helfgott, D, Haider, S, Chandrasekar, P, Langston, A, Perfect, J, Ma, L, Van Lersel, MLPS, Connelly, N, Kartsonis, N, and Waskin, H. "Phase 1b study of new posaconazole tablet for prevention of invasive fungal infections in high-risk patients with neutropenia." Antimicrobial Agents and Chemotherapy 58, no. 10 (October 1, 2014): 5758-5765.

Full Text

Duarte, RF, López-Jiménez, J, Cornely, OA, Laverdiere, M, Helfgott, D, Haider, S, Chandrasekar, P, Langston, A, Perfect, J, Ma, L, van Iersel, MLPS, Connelly, N, Kartsonis, N, and Waskin, H. "Phase 1b study of new posaconazole tablet for prevention of invasive fungal infections in high-risk patients with neutropenia." Antimicrobial agents and chemotherapy 58, no. 10 (October 2014): 5758-5765.

PMID
25049247
Full Text

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