Richard Frothingham, MD

Associate Professor of Medicine
Assistant Professor in Molecular Genetics and Microbiology
Member of the Duke Human Vaccine Institute
Campus mail 2424 Erwin Rd, Hock Plaza Room 9089, Durham, NC 27710
Phone (919) 684-5455
Email address richard.frothingham@duke.edu

Dr. Frothingham is the principal investigator of a research laboratory which studies Mycobacterium tuberculosis, the cause of tuberculosis, and Mycobacterium avium, a closely related bacterium causing serious infections in AIDS patients. We are pursuing two current projects.

The first project aims to develop vaccines against M. avium and M. tuberculosis. We inject mice with candidate plasmid DNA vaccines which produce bacterial proteins in mouse muscle. We use a variety of DNA adjuvants to modify the immune response. We hope to use DNA vaccination to protect against new infections and to modify the course of existing infections. We also hope to identify correlates of vaccine-induced protective immunity.

The second project uses variations in bacterial DNA sequences to identify species and strains. Dr. Frothingham was part of a team of four Duke scientists who used DNA sequence analysis to identify the cause of Whipple's disease. He also identified used DNA sequence to identify a particular group of M. avium strains which cause disseminated infections in AIDS patients. We recently developed a new tuberculosis typing method using variable numbers of tandem DNA repeats. We are applying this new typing method in national and international collaborations.

Dr. Frothingham does not currently conduct clinical trials.

Special areas of expertise include tuberculosis, mycobacteria, strain differentiation, DNA vaccination, and pyrazinamide.

Key words: tuberculosis, mycobacteria, Mycobacterium tuberculosis, Mycobacterium avium, DNA vaccines, tandem repeat DNA, pyrazinamide, mouse

Education and Training

  • Medicine and Pediatrics Resident, Medicine, University of Rochester, 1982 - 1986
  • M.D., Duke University, 1981

Publications

Bifani, P. J., B. Shopsin, P. Alcabes, B. Mathema, B. N. Kreiswirth, Z. Y. Liu, J. Driscoll, R. Frothingham, and J. M. Musser. “Molecular epidemiology and tuberculosis control - Reply.” Jama Journal of the American Medical Association 284, no. 3 (July 19, 2000): 306–7.

Scholars@Duke

Frothingham, R., W. A. Meeker-O’Connell, A. J. Cobb, and S. M. Holland. “Association of Mycobacterium avium sequevars Mav-B and Mav-E with disseminated disease in immunodeficient hosts.” Clinical Infectious Diseases 31, no. 1 (July 1, 2000): 309–309.

Scholars@Duke

Frothingham, R. “Mycobacteria: treatment approaches and mechanisms of resistance..” J Med Liban 48, no. 4 (July 2000): 248–54.

PMID
11214197
Scholars@Duke

Ellingson, J. L., J. R. Stabel, W. R. Bishai, R. Frothingham, and J. M. Miller. “Evaluation of the accuracy and reproducibility of a practical PCR panel assay for rapid detection and differentiation of Mycobacterium avium subspecies..” Mol Cell Probes 14, no. 3 (June 2000): 153–61. https://doi.org/10.1006/mcpr.2000.0299.

PMID
10860713
Full Text

Bifani, P. J., B. Mathema, Z. Liu, S. L. Moghazeh, B. Shopsin, B. Tempalski, J. Driscol, et al. “Identification of a W variant outbreak of Mycobacterium tuberculosis via population-based molecular epidemiology..” Jama 282, no. 24 (December 22, 1999): 2321–27. https://doi.org/10.1001/jama.282.24.2321.

PMID
10612319
Full Text

Cobb, A. J., and R. Frothingham. “The GroEs antigens of Mycobacterium avium and Mycobacterium paratuberculosis.” International Journal of Leprosy and Other Mycobacterial Diseases 67, no. 4 SUPPL. (December 1, 1999).

Scholars@Duke

Velaz Faircloth, M., A. J. Cobb, A. L. Horstman, S. C. Henry, and R. Frothingham. “Protection against Mycobacterium avium by DNA vaccines expressing mycobacterial antigens as fusion proteins with green fluorescent protein.” International Journal of Leprosy and Other Mycobacterial Diseases 67, no. 4 SUPPL. (December 1, 1999).

Scholars@Duke

Talbot, E. A., L. B. Reller, and R. Frothingham. “Bone marrow cultures for the diagnosis of mycobacterial and fungal infections in patients infected with the human immunodeficiency virus..” Int J Tuberc Lung Dis 3, no. 10 (October 1999): 908–12.

PMID
10524589
Scholars@Duke

Yaganehdoost, A., E. A. Graviss, M. W. Ross, G. J. Adams, S. Ramaswamy, A. Wanger, R. Frothingham, H. Soini, and J. M. Musser. “Complex transmission dynamics of clonally related virulent Mycobacterium tuberculosis associated with barhopping by predominantly human immunodeficiency virus-positive gay men..” J Infect Dis 180, no. 4 (October 1999): 1245–51. https://doi.org/10.1086/314991.

PMID
10479154
Full Text

Frothingham, R. “Centrifugation without a balance tube.” American Laboratory 31, no. 19 (September 1, 1999): 10–10.

Scholars@Duke

Pages