Russel E. Kaufman, MD

Professor Emeritus of Medicine
Email address kaufm001@duke.edu

The overall focus of this laboratory is the study of the genetic regulation of normal and leukemic hematopoietic cells. Hematopoietic stem cells are produced during embryonic and fetal development and migrate to fetal liver, spleen, thymus, and bone marrow to populate those organs for the "definitive" stages of hematopoiesis.

We have cloned and determined the structural organization of the human c-kit gene, normally expressed on erythroid progenitors, and identified important regulatory elements in its promoter. Furthermore, we have identified the ets transcription factors as mediators of its signaling pathway and have isolated a novel ets transcription factor, designated PE-2/ERF. Finally, we have cloned and characterized the human GATA-1 and GATA-2 genes and promoters and demonstrate that signaling through the Kit signal transduction pathway has direct effects on these genes, showing that Kit signal transduction in setting up the erythroid developmental program.

This laboratory also has isolated the gene encoding the T cell specific gene CD7, expressed in T cell progenitors. We have determined that the promoter has several motifs similar to the Thy-1 gene and lck gene and present in the murine CD7 gene. We have made transgenic mice carrying the human CD7 gene and find that it is expressed in a pattern similar to the murine gene. These studies and other biochemical studies have helped identify a region of the gene that binds a T cell specific transcription factor. This factor is now being characterized.

Key Words:Hematopoiesis, c-kit, ets, erythropoiesis, leukemia, gene regulation

for more information, see http:marini.biochem.duke.edu/Faculty/Kaufman.html

Education and Training

  • M.D., Ohio State University, 1973

Publications

Wang, Tao, Min Xiao, Yingbin Ge, Clemens Krepler, Eric Belser, Alfonso Lopez-Coral, Xiaowei Xu, et al. “BRAF Inhibition Stimulates Melanoma-Associated Macrophages to Drive Tumor Growth.” Clin Cancer Res 21, no. 7 (April 1, 2015): 1652–64. https://doi.org/10.1158/1078-0432.CCR-14-1554.

PMID
25617424
Full Text

Wang, Tao, Yingbin Ge, Min Xiao, Alfonso Lopez-Coral, Ling Li, Alexander Roesch, Catherine Huang, et al. “SECTM1 produced by tumor cells attracts human monocytes via CD7-mediated activation of the PI3K pathway.” J Invest Dermatol 134, no. 4 (April 2014): 1108–18. https://doi.org/10.1038/jid.2013.437.

PMID
24157461
Full Text

Ge, Y., R. Azuma, B. Gekonge, A. Lopez-Coral, M. Xiao, G. Zhang, X. Xu, et al. “Induction of metallothionein expression during monocyte to melanoma-associated macrophage differentiation.” Frontiers in Biology 7, no. 4 (August 1, 2012): 359–67. https://doi.org/10.1007/s11515-012-1237-8.

Full Text

Wang, Tao, Yingbin Ge, Min Xiao, Alfonso Lopez-Coral, Rikka Azuma, Rajasekharan Somasundaram, Gao Zhang, et al. “Melanoma-derived conditioned media efficiently induce the differentiation of monocytes to macrophages that display a highly invasive gene signature.” Pigment Cell Melanoma Res 25, no. 4 (July 2012): 493–505. https://doi.org/10.1111/j.1755-148X.2012.01005.x.

PMID
22498258
Full Text

Wang, Tao, Catherine Huang, Alfonso Lopez-Coral, Kimberly A. Slentz-Kesler, Min Xiao, E John Wherry, and Russel E. Kaufman. “K12/SECTM1, an interferon-γ regulated molecule, synergizes with CD28 to costimulate human T cell proliferation.” J Leukoc Biol 91, no. 3 (March 2012): 449–59. https://doi.org/10.1189/jlb.1011498.

PMID
22184754
Full Text

Patel, A. A., J. R. Gilbertson, L. C. Showe, J. W. London, E. Ross, M. F. Ochs, J. Carver, et al. “A novel cross-disciplinary multi-institute approach to translational cancer research: Lessons learned from Pennsylvania Cancer Alliance Bioinformatics Consortium (PCABC).” Cancer Informatics 3 (December 1, 2007): 255–74.

Scholars@Duke

Lam, Gordon K., Hua-Xin Liao, Yan Xue, S Munir Alam, Richard M. Scearce, Russel E. Kaufman, Gregory D. Sempowski, and Barton F. Haynes. “Expression of the CD7 ligand K-12 in human thymic epithelial cells: regulation by IFN-gamma.” J Clin Immunol 25, no. 1 (January 2005): 41–49. https://doi.org/10.1007/s10875-005-0356-5.

PMID
15742156
Full Text

Danforth, W., J. T. Bigger, F. Davidoff, M. J. Delaney, S. Dentzer, S. H. Ehringhaus, G. Graham, et al. “Protecting subjects, preserving trust, promoting progress I: Policy and guidelines for the oversight of individual financial interests in human subjects research.” Academic Medicine 78, no. 2 (February 1, 2003): 226–36.

Scholars@Duke

Pages