S. Munir Alam, PhD

Professor in Medicine
Professor of Pathology
Member of the Duke Human Vaccine Institute
Campus mail 2 Genome Ct, MSRB II - Room 4004, Durham, NC 27710
Phone (919) 668-6372
Email address alam0004@mc.duke.edu

Research Interests.
Biophysical analysis of coreceptor modulation of TCR-MHC interactions. One of our research interests is to study the molecular mechanisms of T cell recognition. We have particular interest in understanding the trimolecular interactions between membrane bound T cell receptor (TCR-CD3 complex) and its ligand, the peptide-MHC complex (pMHC), and co-receptor molecules. We are using different biophysical approaches which include surface plasmon resonance, isothermal titration calorimetry and atomic force spectroscopy to study the influence of co-stimulatory molecules on the kinetics and thermodynamics of TCR-pMHC binding. The goal is to use integrated molecular and biophysical approaches to study membrane TCR-MHC interactions and to understand how these interactions are modulated by co-receptors during T cell development and activation.
Energetics and kinetics of monoclonal antibody binding to soluble HIV Envelope proteins. An additional interest of our lab involves biophysical characterization of soluble, recombinant human HIV-1 Envelope proteins (gp120 and gp140). We have employed surface plasmon resonance, analytical sedimentation equilibrium analysis, isothermal titration calorimetry and atomic force spectroscopy to characterize the antigenicity and study the energetics, kinetics and single molecule force measurements of the binding of anti-gp120 and anti-gp41 antibodies to several recombinant HIV-1 Envelope proteins. These studies have relevance in immunogen design for generating anti-HIV vaccines.

Education and Training

  • Ph.D., University of Glasgow (Scotland), 1992

Publications

Bonsignori, M, Zhou, T, Sheng, Z, Chen, L, Gao, F, Joyce, MG, Ozorowski, G, Chuang, G-Y, Schramm, CA, Wiehe, K, Alam, SM, Bradley, T, Gladden, MA, Hwang, K-K, Iyengar, S, Kumar, A, Lu, X, Luo, K, Mangiapani, MC, Parks, RJ, Song, H, Acharya, P, Bailer, RT, Cao, A, Druz, A, Georgiev, IS, Kwon, YD, Louder, MK, Zhang, B, Zheng, A, Hill, BJ, Kong, R, Soto, C, NISC Comparative Sequencing Program, , Mullikin, JC, Douek, DC, Montefiori, DC, Moody, MA, Shaw, GM, Hahn, BH, Kelsoe, G, Hraber, PT, Korber, BT, Boyd, SD, Fire, AZ, Kepler, TB, Shapiro, L, Ward, AB, Mascola, JR, Liao, H-X, Kwong, PD, and Haynes, BF. "Maturation Pathway from Germline to Broad HIV-1 Neutralizer of a CD4-Mimic Antibody." Cell 165, no. 2 (April 2016): 449-463.

PMID
26949186
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Zhang, R, Verkoczy, L, Wiehe, K, Munir Alam, S, Nicely, NI, Santra, S, Bradley, T, Pemble, CW, Zhang, J, Gao, F, Montefiori, DC, Bouton-Verville, H, Kelsoe, G, Larimore, K, Greenberg, PD, Parks, R, Foulger, A, Peel, JN, Luo, K, Lu, X, Trama, AM, Vandergrift, N, Tomaras, GD, Kepler, TB, Moody, MA, Liao, H-X, and Haynes, BF. "Initiation of immune tolerance-controlled HIV gp41 neutralizing B cell lineages." Science Translational Medicine 8, no. 336 (April 2016): 336ra62-null.

PMID
27122615
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Wang, Y, Kapoor, P, Parks, R, Silva-Sanchez, A, Alam, SM, Verkoczy, L, Liao, H-X, Zhuang, Y, Burrows, P, Levinson, M, Elgavish, A, Cui, X, Haynes, BF, and Schroeder, H. "HIV-1 gp140 epitope recognition is influenced by immunoglobulin DH gene segment sequence." Immunogenetics 68, no. 2 (February 2016): 145-155.

PMID
26687685
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Soto, C, Ofek, G, Joyce, MG, Zhang, B, McKee, K, Longo, NS, Yang, Y, Huang, J, Parks, R, Eudailey, J, Lloyd, KE, Alam, SM, Haynes, BF, NISC Comparative Sequencing Program, , Mullikin, JC, Connors, M, Mascola, JR, Shapiro, L, and Kwong, PD. "Developmental Pathway of the MPER-Directed HIV-1-Neutralizing Antibody 10E8." PloS one 11, no. 6 (January 2016): e0157409-.

PMID
27299673
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Bradley, T, Fera, D, Bhiman, J, Eslamizar, L, Lu, X, Anasti, K, Zhang, R, Sutherland, LL, Scearce, RM, Bowman, CM, Stolarchuk, C, Lloyd, KE, Parks, R, Eaton, A, Foulger, A, Nie, X, Karim, SSA, Barnett, S, Kelsoe, G, Kepler, TB, Alam, SM, Montefiori, DC, Moody, MA, Liao, H-X, Morris, L, Santra, S, Harrison, SC, and Haynes, BF. "Structural Constraints of Vaccine-Induced Tier-2 Autologous HIV Neutralizing Antibodies Targeting the Receptor-Binding Site." Cell Reports 14, no. 1 (January 2016): 43-54.

PMID
26725118
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Mansour, RG, Stamper, L, Jaeger, F, McGuire, E, Fouda, G, Amos, J, Barbas, K, Ohashi, T, Alam, SM, Erickson, H, and Permar, SR. "The Presence and Anti-HIV-1 Function of Tenascin C in Breast Milk and Genital Fluids." Plos One 11, no. 5 (January 2016): e0155261-null.

PMID
27182834
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Hraber, P, Korber, B, Wagh, K, Giorgi, EE, Bhattacharya, T, Gnanakaran, S, Lapedes, AS, Learn, GH, Kreider, EF, Li, Y, Shaw, GM, Hahn, BH, Montefiori, DC, Alam, SM, Bonsignori, M, Moody, MA, Liao, H-X, Gao, F, and Haynes, BF. "Longitudinal Antigenic Sequences and Sites from Intra-Host Evolution (LASSIE) Identifies Immune-Selected HIV Variants." Viruses 7, no. 10 (October 21, 2015): 5443-5475.

PMID
26506369
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Moody, MA, Gao, F, Gurley, TC, Amos, JD, Kumar, A, Hora, B, Marshall, DJ, Whitesides, JF, Xia, S-M, Parks, R, Lloyd, KE, Hwang, K-K, Lu, X, Bonsignori, M, Finzi, A, Vandergrift, NA, Alam, SM, Ferrari, G, Shen, X, Tomaras, GD, Kamanga, G, Cohen, MS, Sam, NE, Kapiga, S, Gray, ES, Tumba, NL, Morris, L, Zolla-Pazner, S, Gorny, MK, Mascola, JR, Hahn, BH, Shaw, GM, Sodroski, JG, Liao, H-X, Montefiori, DC, Hraber, PT, Korber, BT, and Haynes, BF. "Strain-Specific V3 and CD4 Binding Site Autologous HIV-1 Neutralizing Antibodies Select Neutralization-Resistant Viruses." Cell Host & Microbe 18, no. 3 (September 2015): 354-362.

PMID
26355218
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Santra, S, Tomaras, GD, Warrier, R, Nicely, NI, Liao, H-X, Pollara, J, Liu, P, Alam, SM, Zhang, R, Cocklin, SL, Shen, X, Duffy, R, Xia, S-M, Schutte, RJ, Pemble Iv, CW, Dennison, SM, Li, H, Chao, A, Vidnovic, K, Evans, A, Klein, K, Kumar, A, Robinson, J, Landucci, G, Forthal, DN, Montefiori, DC, Kaewkungwal, J, Nitayaphan, S, Pitisuttithum, P, Rerks-Ngarm, S, Robb, ML, Michael, NL, Kim, JH, Soderberg, KA, Giorgi, EE, Blair, L, Korber, BT, Moog, C, Shattock, RJ, Letvin, NL, Schmitz, JE, Moody, MA, Gao, F, Ferrari, G, Shaw, GM, and Haynes, BF. "Human Non-neutralizing HIV-1 Envelope Monoclonal Antibodies Limit the Number of Founder Viruses during SHIV Mucosal Infection in Rhesus Macaques." Plos Pathogens 11, no. 8 (August 3, 2015): e1005042-null.

PMID
26237403
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Wieczorek, L, Krebs, SJ, Kalyanaraman, V, Whitney, S, Tovanabutra, S, Moscoso, CG, Sanders-Buell, E, Williams, C, Slike, B, Molnar, S, Dussupt, V, Alam, SM, Chenine, A-L, Tong, T, Hill, EL, Liao, H-X, Hoelscher, M, Maboko, L, Zolla-Pazner, S, Haynes, BF, Pensiero, M, McCutchan, F, Malek-Salehi, S, Cheng, RH, Robb, ML, VanCott, T, Michael, NL, Marovich, MA, Alving, CR, Matyas, GR, Rao, M, and Polonis, VR. "Comparable Antigenicity and Immunogenicity of Oligomeric Forms of a Novel, Acute HIV-1 Subtype C gp145 Envelope for Use in Preclinical and Clinical Vaccine Research." Journal of Virology 89, no. 15 (August 2015): 7478-7493.

PMID
25972551
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