Christina Eleanor Barkauskas, MD

Assistant Professor of Medicine
Affiliate of the Regeneration Next Initiative
Campus mail Box 2629 Med Ctr, Durham, NC 27710
Phone (919) 681-6845
Email address christina.barkauskas@duke.edu

I am a physician-scientist and stem cell biologist focused on understanding the fundamental mechanisms involved in lung injury and repair. I am particularly interested in determining the critical components of the alveolar epithelial stem cell niche and the cellular crosstalk signals that dictate cell identity and behavior during steady state maintenance and repair after injury and infection. I believe this work is fundamental to better understanding the biology of the human lung and the pathogenesis of idiopathic pulmonary fibrosis (IPF), a disease that is characterized by relentless scar accumulation in the lung with only minimally effective therapy short of lung transplant.

Through my work, I have developed several areas of expertise which include the following: 1) Development and use of mouse models to genetically manipulate and lineage trace specific cell populations; 2) Isolation and characterization of murine lung cell subpopulations by flow cytometry; 3) Development and use of an ex vivo 3-dimensional organoid assay to study the self-renewal and differentiation of murine alveolar epithelial stem cells and their niche components; 4) Isolation and 3-dimensional culture of alveolar epithelial cells from fresh human lung; 5) Development and analysis of models of experimental lung fibrosis.

Education and Training

  • Advanced Training, Pulmonary & Critical Care Medicine, Duke University School of Medicine, 2011 - 2012
  • Fellowship, Pulmonary& Critical Care Medicine, Duke University School of Medicine, 2009 - 2011
  • Chief Medical Resident, Department Of Medicine, Duke University School of Medicine, 2008 - 2009
  • Fellow in Pulmonary Medicine, Medicine, Duke University, 2007 - 2008
  • Resident, Medicine, Duke University, 2004 - 2007
  • M.D., Duke University School of Medicine, 2004

Publications

Barkauskas, CE, Chung, M-I, Fioret, B, Gao, X, Katsura, H, and Hogan, BLM. "Lung organoids: current uses and future promise." Development (Cambridge, England) 144, no. 6 (March 2017): 986-997. (Review)

PMID
28292845
Full Text

Wansleeben, C, Barkauskas, CE, Rock, JR, and Hogan, BLM. "Erratum: Stem cells of the adult lung: Their development and role in homeostasis, regeneration, and disease [WIREs Dev Biol, 2, (2013), 131-148] doi: 10.1002/wdev.58." Wiley Interdisciplinary Reviews: Developmental Biology 4, no. 3 (May 1, 2015): 311-312.

Full Text

Fedeles, SV, So, J-S, Shrikhande, A, Lee, SH, Gallagher, A-R, Barkauskas, CE, Somlo, S, and Lee, A-H. "Sec63 and Xbp1 regulate IRE1α activity and polycystic disease severity." The Journal of clinical investigation 125, no. 5 (May 2015): 1955-1967.

PMID
25844898
Full Text

Jain, R, Barkauskas, CE, Takeda, N, Bowie, EJ, Aghajanian, H, Wang, Q, Padmanabhan, A, Manderfield, LJ, Gupta, M, Li, D, Li, L, Trivedi, CM, Hogan, BLM, and Epstein, JA. "Plasticity of Hopx(+) type I alveolar cells to regenerate type II cells in the lung." Nature communications 6 (April 13, 2015): 6727-.

PMID
25865356
Full Text

Alder, JK, Barkauskas, CE, Limjunyawong, N, Stanley, SE, Kembou, F, Tuder, RM, Hogan, BLM, Mitzner, W, and Armanios, M. "Telomere dysfunction causes alveolar stem cell failure." Proceedings of the National Academy of Sciences of the United States of America 112, no. 16 (April 3, 2015): 5099-5104.

PMID
25840590
Full Text

Hogan, BLM, Barkauskas, CE, Chapman, HA, Epstein, JA, Jain, R, Hsia, CCW, Niklason, L, Calle, E, Le, A, Randell, SH, Rock, J, Snitow, M, Krummel, M, Stripp, BR, Vu, T, White, ES, Whitsett, JA, and Morrisey, EE. "Repair and regeneration of the respiratory system: complexity, plasticity, and mechanisms of lung stem cell function." Cell stem cell 15, no. 2 (August 2014): 123-138. (Review)

PMID
25105578
Full Text

Barkauskas, CE, and Noble, PW. "Cellular mechanisms of tissue fibrosis. 7. New insights into the cellular mechanisms of pulmonary fibrosis." American journal of physiology. Cell physiology 306, no. 11 (June 2014): C987-C996. (Review)

PMID
24740535
Full Text

Barkauskas, CE, Cronce, MJ, Rackley, CR, Bowie, EJ, Keene, DR, Stripp, BR, Randell, SH, Noble, PW, and Hogan, BLM. "Type 2 alveolar cells are stem cells in adult lung." J Clin Invest 123, no. 7 (July 2013): 3025-3036.

PMID
23921127
Full Text

Wansleeben, C, Barkauskas, CE, Rock, JR, and Hogan, BLM. "Stem cells of the adult lung: their development and role in homeostasis, regeneration, and disease." Wiley Interdiscip Rev Dev Biol 2, no. 1 (January 2013): 131-148. (Review)

PMID
23799633
Full Text

Noble, PW, Barkauskas, CE, and Jiang, D. "Pulmonary fibrosis: patterns and perpetrators." J Clin Invest 122, no. 8 (August 2012): 2756-2762. (Review)

PMID
22850886
Full Text

Pages