Christina Eleanor Barkauskas, MD

Assistant Professor of Medicine
Affiliate of the Regeneration Next Initiative
Campus mail Box 2629 Med Ctr, Durham, NC 27710
Phone (919) 681-6845
Email address christina.barkauskas@duke.edu

I am a physician-scientist and stem cell biologist focused on understanding the fundamental mechanisms involved in lung injury and repair. I am particularly interested in determining the critical components of the alveolar epithelial stem cell niche and the cellular crosstalk signals that dictate cell identity and behavior during steady state maintenance and repair after injury and infection. I believe this work is fundamental to better understanding the biology of the human lung and the pathogenesis of idiopathic pulmonary fibrosis (IPF), a disease that is characterized by relentless scar accumulation in the lung with only minimally effective therapy short of lung transplant.

Through my work, I have developed several areas of expertise which include the following: 1) Development and use of mouse models to genetically manipulate and lineage trace specific cell populations; 2) Isolation and characterization of murine lung cell subpopulations by flow cytometry; 3) Development and use of an ex vivo 3-dimensional organoid assay to study the self-renewal and differentiation of murine alveolar epithelial stem cells and their niche components; 4) Isolation and 3-dimensional culture of alveolar epithelial cells from fresh human lung; 5) Development and analysis of models of experimental lung fibrosis.

Education and Training

  • Advanced Training, Pulmonary & Critical Care Medicine, Duke University School of Medicine, 2011 - 2012
  • Fellowship, Pulmonary& Critical Care Medicine, Duke University School of Medicine, 2009 - 2011
  • Chief Medical Resident, Department Of Medicine, Duke University School of Medicine, 2008 - 2009
  • Fellow in Pulmonary Medicine, Medicine, Duke University, 2007 - 2008
  • Resident, Medicine, Duke University, 2004 - 2007
  • M.D., Duke University School of Medicine, 2004

Publications

Soliman, Hesham, Ben Paylor, R Wilder Scott, Dario R. Lemos, ChihKai Chang, Martin Arostegui, Marcela Low, et al. “Pathogenic Potential of Hic1-Expressing Cardiac Stromal Progenitors.” Cell Stem Cell 26, no. 3 (March 5, 2020): 459–61. https://doi.org/10.1016/j.stem.2020.01.023.

PMID
32142665
Full Text

Soliman, Hesham, Ben Paylor, R Wilder Scott, Dario R. Lemos, ChihKai Chang, Martin Arostegui, Marcela Low, et al. “Pathogenic Potential of Hic1-Expressing Cardiac Stromal Progenitors.” Cell Stem Cell 26, no. 2 (February 6, 2020): 205-220.e8. https://doi.org/10.1016/j.stem.2019.12.008.

PMID
31978365
Full Text

Glisinski, Kristen M., Adam J. Schlobohm, Sarah V. Paramore, Anastasiya Birukova, M Arthur Moseley, Matthew W. Foster, and Christina E. Barkauskas. “Interleukin-13 disrupts type 2 pneumocyte stem cell activity.” Jci Insight 5, no. 1 (January 16, 2020). https://doi.org/10.1172/jci.insight.131232.

PMID
31941839
Full Text

Loriaux, Daniel, Stephen P. Bergin, Sweta M. Patel, Jesse Tucker, and Christina E. Barkauskas. “Ethylene Glycol Toxicity in the Setting of Recurrent Ingestion: A Case Report and Literature Review.” Cureus 11, no. 4 (April 3, 2019): e4375. https://doi.org/10.7759/cureus.4375.

PMID
31218140
Full Text

Tighe, R. M., A. Birukova, A. Schlobohm, and C. E. Barkauskas. “Delayed Increases in Hyaluronan Following Ozone Exposure Associates with Alveolar Type 2 Cell Proliferation.” In American Journal of Respiratory and Critical Care Medicine, Vol. 199. AMER THORACIC SOC, 2019.

Scholars@Duke

Bates, C. A., B. Zhao, A. Schlobohm, C. Asquith, W. Zuercher, and C. E. Barkauskas. “Inhibition of Cyclin G-Associated Kinase (GAK) Impairs Growth of Alveolar Type 2 Cells in a 3-Dimensional Culture System.” In American Journal of Respiratory and Critical Care Medicine, Vol. 199. AMER THORACIC SOC, 2019.

Scholars@Duke

Chung, Mei-I, Melissa Bujnis, Christina E. Barkauskas, Yoshihiko Kobayashi, and Brigid L. M. Hogan. “Niche-mediated BMP/SMAD signaling regulates lung alveolar stem cell proliferation and differentiation.” Development 145, no. 9 (May 11, 2018). https://doi.org/10.1242/dev.163014.

PMID
29752282
Full Text

Barkauskas, Christina E., Mei-I Chung, Bryan Fioret, Xia Gao, Hiroaki Katsura, and Brigid L. M. Hogan. “Lung organoids: current uses and future promise.” Development 144, no. 6 (March 15, 2017): 986–97. https://doi.org/10.1242/dev.140103.

PMID
28292845
Full Text

Fedeles, Sorin V., Jae-Seon So, Amol Shrikhande, Seung Hun Lee, Anna-Rachel Gallagher, Christina E. Barkauskas, Stefan Somlo, and Ann-Hwee Lee. “Sec63 and Xbp1 regulate IRE1α activity and polycystic disease severity.” J Clin Invest 125, no. 5 (May 2015): 1955–67. https://doi.org/10.1172/JCI78863.

PMID
25844898
Full Text

Pages