David Stephen Pisetsky, MD, PhD

Professor of Medicine
Professor of Immunology
Member of the Duke Cancer Institute
Member of the Duke Human Vaccine Institute
Campus mail VA Med Ctr, 508 Fulton St, Durham, NC 27705
Phone (919) 286-6835
Email address david.pisetsky@duke.edu

Studies in my laboratory concern the immunological properties of DNA as related to two main topics: 1) the induction of anti-DNA responses in systemic lupus erythematosus; and 2) the stimulation of innate immunity by bacterial DNA. These topics are closely linked since we have established novel disease models in which lupus-like illness can be induced in normal mice by bacterial DNA under conditions in which mammalian DNA is inactive. This model has been useful in elucidating mechanisms of DNA antigen drive in autoimmunity.

To pursue these studies, our laboratory conducts investigations in the following areas: 1) specificity of anti-DNA for epitopes on mammalian and bacterial DNA; 2) molecular analysis of murine mononclonal anti-DNA antibodies; 3) histopathological analyses of DNA-immunized mice; 4) in vitro analysis of proliferation, antibody production and cytokine expression in human and murine immune cells; and 5) analysis of DNA binding to cell surface molecules on B cells, T cells and macrophages. Results of these studies have allowed identification of at least two structural motifs that are immunoactive. We are also exploring the impact of chemical modifications of the DNA backbone.

In addition to work on the immunology of DNA, I am also involved in clinical trials related to new immunomodulatory agents in the treatment of rheumatoid arthritis as well as serological markers of disease activity.

The areas of research for which I am known nationally are anti-DNA antibodies, systemic lupus erythematosus and immunological properties of DNA. I have written textbook chapters and reviews on all these subjects.

Education and Training

  • Resident, Medicine, Yale University School of Medicine, 1974 - 1974
  • Intern, Medicine, Yale University School of Medicine, 1973 - 1974
  • M.D., Yeshiva University, Albert Einstein College of Medicine, 1973
  • Ph.D., Yeshiva University, Albert Einstein College of Medicine, 1972


Pisetsky, David S., Angel Garza Reyna, Morgan E. Belina, and Diane M. Spencer. “The Interaction of Anti-DNA Antibodies with DNA: Evidence for Unconventional Binding Mechanisms.” Int J Mol Sci 23, no. 9 (May 7, 2022). https://doi.org/10.3390/ijms23095227.

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Dvergsten, Jeffrey A., Ann M. Reed, Lawrence Landerman, David S. Pisetsky, Olga Ilkayeva, and Kim M. Huffman. “Metabolomics analysis identifies a lipidomic profile in treatment-naïve juvenile dermatomyositis patients vs healthy control subjects.” Rheumatology (Oxford) 61, no. 4 (April 11, 2022): 1699–1708. https://doi.org/10.1093/rheumatology/keab520.

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Belina, Morgan E., Diane M. Spencer, and David S. Pisetsky. “The Binding Mechanisms of Antibodies to DNA from Healthy Subjects and Patients with Systemic Lupus Erythematosus: The Role of Monogamous Bivalency and Fc Dependence.” Immunohorizons 5, no. 10 (October 8, 2021): 792–801. https://doi.org/10.4049/immunohorizons.2100077.

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Pisetsky, David S., Robert Shaffer, Dustin D. Armstrong, and Diane M. Spencer. “The interaction of anti-DNA antibodies with DNA antigen: Evidence for hysteresis for high avidity binding.” Clin Immunol 231 (October 2021): 108848. https://doi.org/10.1016/j.clim.2021.108848.

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Spencer, Diane M., Angel Garza Reyna, and David S. Pisetsky. “The Binding of Monoclonal and Polyclonal Anti-Z-DNA Antibodies to DNA of Various Species Origin.” Int J Mol Sci 22, no. 16 (August 19, 2021). https://doi.org/10.3390/ijms22168931.

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Pisetsky, David S., Amanda M. Eudy, Megan E. B. Clowse, and Jennifer L. Rogers. “The Categorization of Pain in Systemic Lupus Erythematosus.” Rheum Dis Clin North Am 47, no. 2 (May 2021): 215–28. https://doi.org/10.1016/j.rdc.2020.12.004.

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