David Stephen Pisetsky, MD, PhD

Professor of Medicine
Professor of Immunology
Member of the Duke Cancer Institute
Member of the Duke Human Vaccine Institute
Campus mail VA Med Ctr, 508 Fulton St, Durham, NC 27705
Phone (919) 286-6835
Email address david.pisetsky@duke.edu

Studies in my laboratory concern the immunological properties of DNA as related to two main topics: 1) the induction of anti-DNA responses in systemic lupus erythematosus; and 2) the stimulation of innate immunity by bacterial DNA. These topics are closely linked since we have established novel disease models in which lupus-like illness can be induced in normal mice by bacterial DNA under conditions in which mammalian DNA is inactive. This model has been useful in elucidating mechanisms of DNA antigen drive in autoimmunity.

To pursue these studies, our laboratory conducts investigations in the following areas: 1) specificity of anti-DNA for epitopes on mammalian and bacterial DNA; 2) molecular analysis of murine mononclonal anti-DNA antibodies; 3) histopathological analyses of DNA-immunized mice; 4) in vitro analysis of proliferation, antibody production and cytokine expression in human and murine immune cells; and 5) analysis of DNA binding to cell surface molecules on B cells, T cells and macrophages. Results of these studies have allowed identification of at least two structural motifs that are immunoactive. We are also exploring the impact of chemical modifications of the DNA backbone.

In addition to work on the immunology of DNA, I am also involved in clinical trials related to new immunomodulatory agents in the treatment of rheumatoid arthritis as well as serological markers of disease activity.

The areas of research for which I am known nationally are anti-DNA antibodies, systemic lupus erythematosus and immunological properties of DNA. I have written textbook chapters and reviews on all these subjects.

Education and Training

  • M.D., Albert Einstein College of Medicine of Yeshiva University, 1973
  • Ph.D., Albert Einstein College of Medicine of Yeshiva University, 1972

Publications

Pisetsky, DS. "Advances in the Treatment of Rheumatoid Arthritis: Costs and Challenges." North Carolina medical journal 78, no. 5 (September 2017): 337-340.

PMID
28963273
Full Text

Pisetsky, DS. "EULAR recommendations for disease management: guidance not guidelines." Annals of the rheumatic diseases 76, no. 6 (June 2017): 935-938.

PMID
28465346
Full Text

Borenstein, DG, Hassett, AL, and Pisetsky, DS. "Pain management in rheumatology research, training, and practice." Clinical and Experimental Rheumatology 35, no. 5 (January 1, 2017): S2-S7.

Scholars@Duke

Pisetsky, DS. "Mechanisms of Chromatin Remodeling and Repurposing During Extracellular Translocation." Advances in protein chemistry and structural biology 106 (January 2017): 113-137. (Review)

PMID
28057209
Full Text

Schett, G, Emery, P, Tanaka, Y, Burmester, G, Pisetsky, DS, Naredo, E, Fautrel, B, and van Vollenhoven, R. "Tapering biologic and conventional DMARD therapy in rheumatoid arthritis: current evidence and future directions." Annals of the rheumatic diseases 75, no. 8 (August 2016): 1428-1437. (Review)

PMID
27261493
Full Text

Mobarrez, F, Abraham-Nordling, M, Aguilera-Gatica, K, Friberg, I, Antovic, A, Pisetsky, DS, Jörneskog, G, and Wallen, H. "The expression of microvesicles in the blood of patients with Graves' disease and its relationship to treatment." Clinical endocrinology 84, no. 5 (May 2016): 729-735.

PMID
26252432
Full Text

Magna, M, and Pisetsky, DS. "The Alarmin Properties of DNA and DNA-associated Nuclear Proteins." Clinical therapeutics 38, no. 5 (May 2016): 1029-1041. (Review)

PMID
27021604
Full Text

Stearns, NA, and Pisetsky, DS. "The role of monogamous bivalency and Fc interactions in the binding of anti-DNA antibodies to DNA antigen." Clinical immunology (Orlando, Fla.) 166-167 (May 2016): 38-47.

PMID
27083935
Full Text

Pisetsky, DS. "Anti-DNA antibodies--quintessential biomarkers of SLE." Nature reviews. Rheumatology 12, no. 2 (February 2016): 102-110. (Review)

PMID
26581343
Full Text

Stearns, NA, Zhou, S, Petri, M, Binder, SR, and Pisetsky, DS. "The Use of Poly-L-Lysine as a Capture Agent to Enhance the Detection of Antinuclear Antibodies by ELISA." PloS one 11, no. 9 (January 2016): e0161818-.

PMID
27611194
Full Text

Pages