David Stephen Pisetsky, MD, PhD

Professor of Medicine
Professor of Immunology
Member of the Duke Cancer Institute
Member of the Duke Human Vaccine Institute
Campus mail VA Med Ctr, 508 Fulton St, Durham, NC 27705
Phone (919) 286-6835
Email address david.pisetsky@duke.edu

Studies in my laboratory concern the immunological properties of DNA as related to two main topics: 1) the induction of anti-DNA responses in systemic lupus erythematosus; and 2) the stimulation of innate immunity by bacterial DNA. These topics are closely linked since we have established novel disease models in which lupus-like illness can be induced in normal mice by bacterial DNA under conditions in which mammalian DNA is inactive. This model has been useful in elucidating mechanisms of DNA antigen drive in autoimmunity.

To pursue these studies, our laboratory conducts investigations in the following areas: 1) specificity of anti-DNA for epitopes on mammalian and bacterial DNA; 2) molecular analysis of murine mononclonal anti-DNA antibodies; 3) histopathological analyses of DNA-immunized mice; 4) in vitro analysis of proliferation, antibody production and cytokine expression in human and murine immune cells; and 5) analysis of DNA binding to cell surface molecules on B cells, T cells and macrophages. Results of these studies have allowed identification of at least two structural motifs that are immunoactive. We are also exploring the impact of chemical modifications of the DNA backbone.

In addition to work on the immunology of DNA, I am also involved in clinical trials related to new immunomodulatory agents in the treatment of rheumatoid arthritis as well as serological markers of disease activity.

The areas of research for which I am known nationally are anti-DNA antibodies, systemic lupus erythematosus and immunological properties of DNA. I have written textbook chapters and reviews on all these subjects.

Education and Training

  • Resident, Medicine, Yale University School of Medicine, 1974 - 1974
  • Intern, Medicine, Yale University School of Medicine, 1973 - 1974
  • M.D., Albert Einstein College of Medicine of Yeshiva University, 1973
  • Ph.D., Albert Einstein College of Medicine of Yeshiva University, 1972

Publications

Pisetsky, David S., Dana Kathryn Thompson, Joseph Wajdula, Annette Diehl, and Sudhakar Sridharan. “Variability in Antinuclear Antibody Testing to Assess Patient Eligibility for Clinical Trials of Novel Treatments for Systemic Lupus Erythematosus..” Arthritis Rheumatol 71, no. 9 (September 2019): 1534–38. https://doi.org/10.1002/art.40910.

PMID
31385442
Full Text

Pisetsky, David S., Diane M. Spencer, Peter E. Lipsky, and Brad H. Rovin. “Response to: 'Antinuclear antibody as entry criterion for classification of systemic lupus erythematosus: pitfalls and opportunities' by Bossuyt et al..” Ann Rheum Dis 78, no. 8 (August 2019). https://doi.org/10.1136/annrheumdis-2018-213841.

PMID
29936440
Full Text

Mobarrez, Fariborz, Enrico Fuzzi, Iva Gunnarsson, Anders Larsson, Susanna Eketjäll, David S. Pisetsky, and Elisabet Svenungsson. “Microparticles in the blood of patients with SLE: Size, content of mitochondria and role in circulating immune complexes..” J Autoimmun 102 (August 2019): 142–49. https://doi.org/10.1016/j.jaut.2019.05.003.

PMID
31103269
Full Text

Pisetsky, David S., Diane M. Spencer, Peter E. Lipsky, and Brad H. Rovin. “Response to: 'Pitfalls of antinuclear antibody detection in systemic lupus erythematosus: the positive experience of a national multi-center study' by Pregnalato et al..” Ann Rheum Dis 78, no. 6 (June 2019). https://doi.org/10.1136/annrheumdis-2018-213582.

PMID
29730639
Full Text

Pisetsky, David S., Diane M. Spencer, Peter E. Lipsky, and Brad H. Rovin. “Response to: 'Variation in antinuclear antibody detection by automated indirect immunofluorescence analysis' by van Hoovels et al..” Ann Rheum Dis 78, no. 6 (June 2019). https://doi.org/10.1136/annrheumdis-2018-213558.

PMID
29730638
Full Text

Pisetsky, David S., Diane M. Spencer, Peter E. Lipsky, and Brad H. Rovin. “Response to: 'Unending story of the indirect immunofluorescence assay on HEp-2 cells: old problems and new solutions?' by Meroni et al..” Ann Rheum Dis 78, no. 6 (June 2019). https://doi.org/10.1136/annrheumdis-2018-213537.

PMID
29705742
Full Text

Pisetsky, David S., Megan E. B. Clowse, Lisa G. Criscione-Schreiber, and Jennifer L. Rogers. “A Novel System to Categorize the Symptoms of Systemic Lupus Erythematosus..” Arthritis Care Res (Hoboken) 71, no. 6 (June 2019): 735–41. https://doi.org/10.1002/acr.23794.

PMID
30354033
Full Text

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