David Stephen Pisetsky, MD, PhD

Professor of Medicine
Professor of Immunology
Member of the Duke Cancer Institute
Member of the Duke Human Vaccine Institute
Campus mail VA Med Ctr, 508 Fulton St, Durham, NC 27705
Phone (919) 286-6835
Email address david.pisetsky@duke.edu

Studies in my laboratory concern the immunological properties of DNA as related to two main topics: 1) the induction of anti-DNA responses in systemic lupus erythematosus; and 2) the stimulation of innate immunity by bacterial DNA. These topics are closely linked since we have established novel disease models in which lupus-like illness can be induced in normal mice by bacterial DNA under conditions in which mammalian DNA is inactive. This model has been useful in elucidating mechanisms of DNA antigen drive in autoimmunity.

To pursue these studies, our laboratory conducts investigations in the following areas: 1) specificity of anti-DNA for epitopes on mammalian and bacterial DNA; 2) molecular analysis of murine mononclonal anti-DNA antibodies; 3) histopathological analyses of DNA-immunized mice; 4) in vitro analysis of proliferation, antibody production and cytokine expression in human and murine immune cells; and 5) analysis of DNA binding to cell surface molecules on B cells, T cells and macrophages. Results of these studies have allowed identification of at least two structural motifs that are immunoactive. We are also exploring the impact of chemical modifications of the DNA backbone.

In addition to work on the immunology of DNA, I am also involved in clinical trials related to new immunomodulatory agents in the treatment of rheumatoid arthritis as well as serological markers of disease activity.

The areas of research for which I am known nationally are anti-DNA antibodies, systemic lupus erythematosus and immunological properties of DNA. I have written textbook chapters and reviews on all these subjects.

Education and Training

  • Resident, Medicine, Yale University School of Medicine, 1974 - 1974
  • Intern, Medicine, Yale University School of Medicine, 1973 - 1974
  • M.D., Albert Einstein College of Medicine of Yeshiva University, 1973
  • Ph.D., Albert Einstein College of Medicine of Yeshiva University, 1972

Publications

Spencer, DM, Dye, JR, Piantadosi, CA, and Pisetsky, DS. "The release of microparticles and mitochondria from RAW 264.7 murine macrophage cells undergoing necroptotic cell death in vitro." Experimental cell research 363, no. 2 (February 2018): 151-159.

PMID
29291399
Full Text

Frank-Bertoncelj, M, Pisetsky, DS, Kolling, C, Michel, BA, Gay, RE, Jüngel, A, and Gay, S. "TLR3 Ligand Poly(I:C) Exerts Distinct Actions in Synovial Fibroblasts When Delivered by Extracellular Vesicles." Frontiers in immunology 9 (January 29, 2018): 28-.

PMID
29434584
Full Text

Pisetsky, DS. "The biological functions of DNA: from the sublime to the slime." Arthritis research & therapy 19, no. 1 (December 15, 2017): 275-.

PMID
29246183
Full Text

Zerwas, S, Larsen, JT, Petersen, L, Thornton, LM, Quaranta, M, Koch, SV, Pisetsky, D, Mortensen, PB, and Bulik, CM. "Eating Disorders, Autoimmune, and Autoinflammatory Disease." Pediatrics 140, no. 6 (December 2017).

PMID
29122972
Full Text

Borenstein, DG, Hassett, AL, and Pisetsky, D. "Pain management in rheumatology research, training, and practice." Clinical and experimental rheumatology 35 Suppl 107, no. 5 (September 28, 2017): 2-7. (Review)

PMID
28967362
Scholars@Duke

Pisetsky, DS. "Advances in the Treatment of Rheumatoid Arthritis: Costs and Challenges." North Carolina medical journal 78, no. 5 (September 2017): 337-340.

PMID
28963273
Full Text

Pisetsky, DS. "Antinuclear antibody testing - misunderstood or misbegotten?." Nature reviews. Rheumatology 13, no. 8 (August 2017): 495-502. (Review)

PMID
28541299
Full Text

Pisetsky, DS. "EULAR recommendations for disease management: guidance not guidelines." Annals of the rheumatic diseases 76, no. 6 (June 2017): 935-938.

PMID
28465346
Full Text

Pisetsky, DS, Rovin, BH, and Lipsky, PE. "New Perspectives in Rheumatology: Biomarkers as Entry Criteria for Clinical Trials of New Therapies for Systemic Lupus Erythematosus: The Example of Antinuclear Antibodies and Anti-DNA." Arthritis & rheumatology (Hoboken, N.J.) 69, no. 3 (March 2017): 487-493.

PMID
27899010
Full Text

Pisetsky, DS. "Mechanisms of Chromatin Remodeling and Repurposing During Extracellular Translocation." Advances in protein chemistry and structural biology 106 (January 2017): 113-137. (Review)

PMID
28057209
Full Text

Pages