David Stephen Pisetsky, MD, PhD

Professor of Medicine
Professor of Immunology
Member of the Duke Cancer Institute
Member of the Duke Human Vaccine Institute
Campus mail VA Med Ctr, 508 Fulton St, Durham, NC 27705
Phone (919) 286-6835
Email address david.pisetsky@duke.edu

Studies in my laboratory concern the immunological properties of DNA as related to two main topics: 1) the induction of anti-DNA responses in systemic lupus erythematosus; and 2) the stimulation of innate immunity by bacterial DNA. These topics are closely linked since we have established novel disease models in which lupus-like illness can be induced in normal mice by bacterial DNA under conditions in which mammalian DNA is inactive. This model has been useful in elucidating mechanisms of DNA antigen drive in autoimmunity.

To pursue these studies, our laboratory conducts investigations in the following areas: 1) specificity of anti-DNA for epitopes on mammalian and bacterial DNA; 2) molecular analysis of murine mononclonal anti-DNA antibodies; 3) histopathological analyses of DNA-immunized mice; 4) in vitro analysis of proliferation, antibody production and cytokine expression in human and murine immune cells; and 5) analysis of DNA binding to cell surface molecules on B cells, T cells and macrophages. Results of these studies have allowed identification of at least two structural motifs that are immunoactive. We are also exploring the impact of chemical modifications of the DNA backbone.

In addition to work on the immunology of DNA, I am also involved in clinical trials related to new immunomodulatory agents in the treatment of rheumatoid arthritis as well as serological markers of disease activity.

The areas of research for which I am known nationally are anti-DNA antibodies, systemic lupus erythematosus and immunological properties of DNA. I have written textbook chapters and reviews on all these subjects.

Education and Training

  • Resident, Medicine, Yale University School of Medicine, 1974 - 1974
  • Intern, Medicine, Yale University School of Medicine, 1973 - 1974
  • M.D., Albert Einstein College of Medicine of Yeshiva University, 1973
  • Ph.D., Albert Einstein College of Medicine of Yeshiva University, 1972

Publications

Pisetsky, DS, Spencer, DM, Lipsky, PE, and Rovin, BH. "Response to 'Antinuclear antibodies by indirect immunofluorescence and solid phase assays' by Bossuyt et al." Annals of the Rheumatic Diseases (May 10, 2019).

PMID
31076389
Full Text

Pisetsky, DS, and Lipsky, PE. "Role of Antinuclear Antibody Determinations in Classification Criteria for Systemic Lupus Erythematosus: Comment on the Article by Leuchten et al." Arthritis Care & Research 71, no. 5 (May 2019): 696-null. (Letter)

PMID
29579355
Full Text

Pisetsky, DS, Spencer, DM, Lipsky, PE, and Rovin, BH. "Response to: 'Lack of standardization of ANA and implications for drug development and precision medicine' by Mahler." Annals of the Rheumatic Diseases 78, no. 5 (May 2019): e34-null.

PMID
29592916
Full Text

Toes, R, and Pisetsky, DS. "Pathogenic effector functions of ACPA: Where do we stand?." Annals of the Rheumatic Diseases (April 20, 2019).

PMID
31005898
Full Text

Pisetsky, DS, Spencer, DM, Lipsky, PE, and Rovin, BH. "Response to: 'Can solid-phase assays replace immunofluorescence for ANA screening?' by Bizzaro." Annals of the Rheumatic Diseases (January 18, 2019).

PMID
30659048
Full Text

Pisetsky, DS. "The central role of nucleic acids in the pathogenesis of systemic lupus erythematosus." F1000research 8 (January 2019). (Review)

PMID
31001416
Full Text

Mobarrez, F, Svenungsson, E, and Pisetsky, DS. "Microparticles as autoantigens in systemic lupus erythematosus." European Journal of Clinical Investigation 48, no. 12 (December 2018): e13010-null.

PMID
30062774
Full Text

Pisetsky, DS, Spencer, DM, Lipsky, PE, and Rovin, B. "Response to: "Antinuclear autoantibodies: discordance among four different assays" by Pacheco et al." Annals of the Rheumatic Diseases (December 2018).

PMID
30504443
Full Text

Pisetsky, DS, Spencer, DM, Lipsky, PE, and Rovin, BH. "Response to: 'ANA testing in "real life"' by Infantino et al." Annals of the Rheumatic Diseases (November 17, 2018).

PMID
30448767
Full Text

Pisetsky, DS, Clowse, MEB, Criscione-Schreiber, LG, and Rogers, JL. "A Novel System to Categorize the Symptoms of Systemic Lupus Erythematosus." Arthritis Care & Research (October 24, 2018). (Review)

PMID
30354033
Full Text

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