David Stephen Pisetsky, MD, PhD

Professor of Medicine
Professor of Immunology
Member of the Duke Cancer Institute
Member of the Duke Human Vaccine Institute
Campus mail VA Med Ctr, 508 Fulton St, Durham, NC 27705
Phone (919) 286-6835
Email address david.pisetsky@duke.edu

Studies in my laboratory concern the immunological properties of DNA as related to two main topics: 1) the induction of anti-DNA responses in systemic lupus erythematosus; and 2) the stimulation of innate immunity by bacterial DNA. These topics are closely linked since we have established novel disease models in which lupus-like illness can be induced in normal mice by bacterial DNA under conditions in which mammalian DNA is inactive. This model has been useful in elucidating mechanisms of DNA antigen drive in autoimmunity.

To pursue these studies, our laboratory conducts investigations in the following areas: 1) specificity of anti-DNA for epitopes on mammalian and bacterial DNA; 2) molecular analysis of murine mononclonal anti-DNA antibodies; 3) histopathological analyses of DNA-immunized mice; 4) in vitro analysis of proliferation, antibody production and cytokine expression in human and murine immune cells; and 5) analysis of DNA binding to cell surface molecules on B cells, T cells and macrophages. Results of these studies have allowed identification of at least two structural motifs that are immunoactive. We are also exploring the impact of chemical modifications of the DNA backbone.

In addition to work on the immunology of DNA, I am also involved in clinical trials related to new immunomodulatory agents in the treatment of rheumatoid arthritis as well as serological markers of disease activity.

The areas of research for which I am known nationally are anti-DNA antibodies, systemic lupus erythematosus and immunological properties of DNA. I have written textbook chapters and reviews on all these subjects.

Education and Training

  • Resident, Medicine, Yale University School of Medicine, 1974 - 1974
  • Intern, Medicine, Yale University School of Medicine, 1973 - 1974
  • M.D., Albert Einstein College of Medicine of Yeshiva University, 1973
  • Ph.D., Albert Einstein College of Medicine of Yeshiva University, 1972


Pisetsky, David S., Diane M. Spencer, Peter E. Lipsky, and Brad H. Rovin. “Response to: 'Can solid-phase assays replace immunofluorescence for ANA screening?' by Bizzaro.” Ann Rheum Dis 79, no. 3 (March 2020): e33. https://doi.org/10.1136/annrheumdis-2018-214829.

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Pisetsky, David S., Diane M. Spencer, Fariborz Mobarrez, Enrico Fuzzi, Iva Gunnarsson, and Elisabet Svenungsson. “The binding of SLE autoantibodies to mitochondria.” Clin Immunol 212 (March 2020): 108349. https://doi.org/10.1016/j.clim.2020.108349.

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Pisetsky, David S., Diane M. Spencer, Peter E. Lipsky, and Brad Rovin. “Response to: "Antinuclear autoantibodies: discordance among four different assays" by Pacheco et al.” Ann Rheum Dis 79, no. 1 (January 2020): e7. https://doi.org/10.1136/annrheumdis-2018-214766.

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Weinmann, Sophia C., and David S. Pisetsky. “Mechanisms of immune-related adverse events during the treatment of cancer with immune checkpoint inhibitors.” Rheumatology (Oxford) 58, no. Supplement_7 (December 1, 2019): vii59–67. https://doi.org/10.1093/rheumatology/kez308.

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Pisetsky, David S., Dana Kathryn Thompson, Joseph Wajdula, Annette Diehl, and Sudhakar Sridharan. “Variability in Antinuclear Antibody Testing to Assess Patient Eligibility for Clinical Trials of Novel Treatments for Systemic Lupus Erythematosus.” Arthritis Rheumatol 71, no. 9 (September 2019): 1534–38. https://doi.org/10.1002/art.40910.

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