John David Hamilton, MD

Professor Emeritus of Medicine
Campus mail Division Of Infectious Diseases, Room 185 Hanes House, Durham, NC 27710
Phone (919) 668-3368
Email address hamil008@mc.duke.edu

1) My laboratory is engaged in studies of viral latency using murine cytomegalovirus, a member of the family of beta-Herpes viruses. Of particular interest in our laboratory, has been the mechanisms of control of latent virus. This question is, we believe, a biologically very important one since it has direct and indirect implications for viral transmission, prevention and treatment of disease.

2) In experiments using reverse transcriptase PCR, we were able to detect immediate-early I transcripts at a level roughly 10-fold higher than that of viral genome, indicating that a significant fraction of latent virus is transcriptionally active. These findings may be indicative of an abortive infection in which transcription is limited to the immediate-early genes, or the result of a very low level persistent infection, or a transient reactivation of latent virus. To further assess the state of viral activity in latent infection we have tested the activity of monoclonal antibodies against putative latent viral proteins. We have further established the gene map location encoding one of these unique proteins and is found to be distinct from the immediate early genes. Further studies using immunohistochemistry indicate that this viral gene is active during latency although not exclusively and therefore not likely to be a regulatory gene for latency. Presently using a recombinant virus containing the gene for green fluorescent protein we will attempt to localize the cellular reservoir latent virus by confocal microscopy.

3) In addition, I have been very active in the conduct of clinical trials relevant to HIV/AIDS. These include a study of early versus late AZT of which I was co-principal investigator in this VA Cooperative Study and I am principal investigator for a pending application to the NIH for an Adult AIDS Clinical Trials Network.

4) I am both a frequent reviewer of manuscripts submitted for publication in the general area of basic and clinical virology and I have been appointed to several national advisory committees including White House Task Force on HIV Vaccine (FCCSET), the Medical Research Advisory Committee of the Department of Veterans Affairs, and the FDA Antiviral Advisory Committee.

5) Key Words
Virus, latency, molecular, cytomegalovirus, immunocompromised, HIV, hepatitis

Education and Training

  • M.D., University of Colorado at Colorado Springs, 1964

Publications

Banks, TA, Rickert, S, Benedict, CA, Ma, L, Ko, M, Meier, J, Ha, W, Schneider, K, Granger, SW, Turovskaya, O, Elewaut, D, Otero, D, French, AR, Henry, SC, Hamilton, JD, Scheu, S, Pfeffer, K, and Ware, CF. "A lymphotoxin-IFN-beta axis essential for lymphocyte survival revealed during cytomegalovirus infection." J Immunol 174, no. 11 (June 1, 2005): 7217-7225.

PMID
15905567
Scholars@Duke

Gopal, IN, Quinn, A, Henry, SC, Hamilton, JD, Staats, HF, and Frothingham, R. "Nasal peptide vaccination elicits CD8 responses and reduces viral burden after challenge with virulent murine cytomegalovirus." Microbiol Immunol 49, no. 2 (2005): 113-119.

PMID
15722596
Scholars@Duke

Weintrob, AC, Hamilton, JD, Hahn, C, Klinzman, D, Moyo, G, Zdunek, D, Hess, G, Benjamin, DK, and Stapleton, JT. "Active or prior GB virus C infection does not protect against vertical transmission of HIV in coinfected women from Tanzania." Clin Infect Dis 38, no. 6 (March 15, 2004): e46-e48.

PMID
14999645
Full Text

Ole-Nguyaine, S, Crump, JA, Kibiki, GS, Kiang, K, Taylor, J, Schimana, W, Bartlett, JA, Shao, JF, Hamilton, JD, and Thielman, NM. "HIV-associated morbidity, mortality and diagnostic testing opportunities among inpatients at a referral hospital in northern Tanzania." Ann Trop Med Parasitol 98, no. 2 (March 2004): 171-179.

PMID
15035727
Full Text

Dalod, M, Hamilton, T, Salomon, R, Salazar-Mather, TP, Henry, SC, Hamilton, JD, and Biron, CA. "Dendritic cell responses to early murine cytomegalovirus infection: subset functional specialization and differential regulation by interferon alpha/beta." J Exp Med 197, no. 7 (April 7, 2003): 885-898.

PMID
12682109
Full Text

Lee, PY, Matchar, DB, Clements, DA, Huber, J, Hamilton, JD, and Peterson, ED. "Economic analysis of influenza vaccination and antiviral treatment for healthy working adults." Ann Intern Med 137, no. 4 (August 20, 2002): 225-231.

PMID
12186512
Scholars@Duke

Henry, SC, Schmader, K, Brown, TT, Miller, SE, Howell, DN, Daley, GG, and Hamilton, JD. "Enhanced green fluorescent protein as a marker for localizing murine cytomegalovirus in acute and latent infection." J Virol Methods 89, no. 1-2 (September 2000): 61-73.

PMID
10996640
Scholars@Duke

Hamilton, JD, and Bridge, J. "Outcome at 6 months for 50 adolescents with major depression treated in a health maintenance organization." J Am Acad Child Adolesc Psychiatry 38, no. 11 (November 1999): 1340-1346.

PMID
10560219
Full Text

Wilson, RM, Harrison, BT, Gibberd, RW, and Hamilton, JD. "An analysis of the causes of adverse events from the Quality in Australian Health Care Study." Med J Aust 170, no. 9 (May 3, 1999): 411-415.

PMID
10341771
Scholars@Duke

Abene, MV, and Hamilton, JD. "Resolution of fear of flying with fluoxetine treatment." J Anxiety Disord 12, no. 6 (November 1998): 599-603.

PMID
9879038
Scholars@Duke

Pages