John David Hamilton, MD

Professor Emeritus of Medicine
Campus mail Division of Infectious Diseases, Room 185 Hanes House, Durham, NC 27710
Phone (919) 668-3368
Email address hamil008@mc.duke.edu

1) My laboratory is engaged in studies of viral latency using murine cytomegalovirus, a member of the family of beta-Herpes viruses. Of particular interest in our laboratory, has been the mechanisms of control of latent virus. This question is, we believe, a biologically very important one since it has direct and indirect implications for viral transmission, prevention and treatment of disease.

2) In experiments using reverse transcriptase PCR, we were able to detect immediate-early I transcripts at a level roughly 10-fold higher than that of viral genome, indicating that a significant fraction of latent virus is transcriptionally active. These findings may be indicative of an abortive infection in which transcription is limited to the immediate-early genes, or the result of a very low level persistent infection, or a transient reactivation of latent virus. To further assess the state of viral activity in latent infection we have tested the activity of monoclonal antibodies against putative latent viral proteins. We have further established the gene map location encoding one of these unique proteins and is found to be distinct from the immediate early genes. Further studies using immunohistochemistry indicate that this viral gene is active during latency although not exclusively and therefore not likely to be a regulatory gene for latency. Presently using a recombinant virus containing the gene for green fluorescent protein we will attempt to localize the cellular reservoir latent virus by confocal microscopy.

3) In addition, I have been very active in the conduct of clinical trials relevant to HIV/AIDS. These include a study of early versus late AZT of which I was co-principal investigator in this VA Cooperative Study and I am principal investigator for a pending application to the NIH for an Adult AIDS Clinical Trials Network.

4) I am both a frequent reviewer of manuscripts submitted for publication in the general area of basic and clinical virology and I have been appointed to several national advisory committees including White House Task Force on HIV Vaccine (FCCSET), the Medical Research Advisory Committee of the Department of Veterans Affairs, and the FDA Antiviral Advisory Committee.

5) Key Words
Virus, latency, molecular, cytomegalovirus, immunocompromised, HIV, hepatitis

Education and Training

  • M.D., University of Colorado at Colorado Springs, 1964

Publications

Merchant, J. A., J. C. Lumsden, K. H. Kilburn, W. M. O’Fallon, J. R. Ujda, V. H. Germino, and J. D. Hamilton. “Dose response studies in cotton textile workers..” J Occup Med 15, no. 3 (March 1973): 222–30.

PMID
4693165
Scholars@Duke

Merchant, J. A., J. C. Lumsden, K. H. Kilburn, W. M. O’Fallon, J. R. Ujda, V. H. Germino, and J. D. Hamilton. “An industrial study of the biological effects of cotton dust and cigarette smoke exposure..” J Occup Med 15, no. 3 (March 1973): 212–21.

PMID
4693164
Scholars@Duke

Hamilton, J. D. “The selection of medical students at McMaster University..” J R Coll Physicians Lond 6, no. 4 (July 1972): 348–51.

PMID
5043437
Scholars@Duke

Merchant, J. A., K. H. Kilburn, W. M. O’Fallon, J. D. Hamilton, and J. C. Lumsden. “Byssinosis and chronic bronchitis among cotton textile workers..” Ann Intern Med 76, no. 3 (March 1972): 423–33. https://doi.org/10.7326/0003-4819-76-3-423.

PMID
5015917
Full Text

Harris, T. R., J. A. Merchant, K. H. Kilburn, and J. D. Hamilton. “Byssinosis and respiratory diseases of cotton mill workers..” J Occup Med 14, no. 3 (March 1972): 199–206.

PMID
4112957
Scholars@Duke

Hamilton, J. D. “The absorption of oleic acid from emulsion and micellar solution..” Biochim Biophys Acta 239, no. 1 (June 8, 1971): 1–8. https://doi.org/10.1016/0005-2760(71)90185-8.

PMID
5569937
Full Text

Hamilton, J. D., N. H. Dyer, A. M. Dawson, F. W. O’Grady, A. Vince, J. C. Fenton, and D. L. Mollin. “Assessment and significance of bacterial overgrowth in the small bowel..” Q J Med 39, no. 154 (April 1970): 265–85.

PMID
5449592
Scholars@Duke

Edwards, A. J., J. D. Hamilton, W. D. Nichol, G. W. Taylor, and A. M. Dawson. “Experience with coeliac axis compression syndrome..” Br Med J 1, no. 5692 (February 7, 1970): 342–45. https://doi.org/10.1136/bmj.1.5692.342.

PMID
5416813
Full Text

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