John David Hamilton, MD

Professor Emeritus of Medicine
Campus mail Division of Infectious Diseases, Room 185 Hanes House, Durham, NC 27710
Phone (919) 668-3368
Email address hamil008@mc.duke.edu

1) My laboratory is engaged in studies of viral latency using murine cytomegalovirus, a member of the family of beta-Herpes viruses. Of particular interest in our laboratory, has been the mechanisms of control of latent virus. This question is, we believe, a biologically very important one since it has direct and indirect implications for viral transmission, prevention and treatment of disease.

2) In experiments using reverse transcriptase PCR, we were able to detect immediate-early I transcripts at a level roughly 10-fold higher than that of viral genome, indicating that a significant fraction of latent virus is transcriptionally active. These findings may be indicative of an abortive infection in which transcription is limited to the immediate-early genes, or the result of a very low level persistent infection, or a transient reactivation of latent virus. To further assess the state of viral activity in latent infection we have tested the activity of monoclonal antibodies against putative latent viral proteins. We have further established the gene map location encoding one of these unique proteins and is found to be distinct from the immediate early genes. Further studies using immunohistochemistry indicate that this viral gene is active during latency although not exclusively and therefore not likely to be a regulatory gene for latency. Presently using a recombinant virus containing the gene for green fluorescent protein we will attempt to localize the cellular reservoir latent virus by confocal microscopy.

3) In addition, I have been very active in the conduct of clinical trials relevant to HIV/AIDS. These include a study of early versus late AZT of which I was co-principal investigator in this VA Cooperative Study and I am principal investigator for a pending application to the NIH for an Adult AIDS Clinical Trials Network.

4) I am both a frequent reviewer of manuscripts submitted for publication in the general area of basic and clinical virology and I have been appointed to several national advisory committees including White House Task Force on HIV Vaccine (FCCSET), the Medical Research Advisory Committee of the Department of Veterans Affairs, and the FDA Antiviral Advisory Committee.

5) Key Words
Virus, latency, molecular, cytomegalovirus, immunocompromised, HIV, hepatitis

Education and Training

  • M.D., University of Colorado at Colorado Springs, 1964

Publications

Oddone, E. Z., P. A. Cowper, J. D. Hamilton, and J. R. Feussner. “A cost-effectiveness analysis of hepatitis B vaccine in predialysis patients..” Health Serv Res 28, no. 1 (April 1993): 97–121.

PMID
8463111
Scholars@Duke

Henry, S. C., and J. D. Hamilton. “Detection of murine cytomegalovirus immediate early 1 transcripts in the spleens of latently infected mice..” J Infect Dis 167, no. 4 (April 1993): 950–54. https://doi.org/10.1093/infdis/167.4.950.

PMID
8383725
Full Text

Simberkoff, M. S., P. M. Hartigan, J. D. Hamilton, D. Deykin, M. Gail, J. G. Bartlett, P. Feorino, R. Redfield, R. Roberts, and D. Collins. “Ethical dilemmas in continuing a zidovudine trial after early termination of similar trials..” Control Clin Trials 14, no. 1 (February 1993): 6–18. https://doi.org/10.1016/0197-2456(93)90046-g.

PMID
8095008
Full Text

Hamilton, J. D., T. J. Courville, B. Richman, P. Hanson, C. Swanson, and J. Stafford. “Quality assessment and improvement in group psychotherapy..” Am J Psychiatry 150, no. 2 (February 1993): 316–20. https://doi.org/10.1176/ajp.150.2.316.

PMID
8422084
Full Text

Sanders, A. R., D. E. Rincon-Limas, R. Chakraborty, B. Grandchamp, J. D. Hamilton, W. E. Fann, and P. I. Patel. “Association between genetic variation at the porphobilinogen deaminase gene and schizophrenia..” Schizophr Res 8, no. 3 (January 1993): 211–21. https://doi.org/10.1016/0920-9964(93)90019-f.

PMID
8094629
Full Text

Schmader, K. E., R. Rahija, K. R. Porter, G. Daley, and J. D. Hamilton. “Aging and reactivation of latent murine cytomegalovirus..” J Infect Dis 166, no. 6 (December 1992): 1403–7. https://doi.org/10.1093/infdis/166.6.1403.

PMID
1331252
Full Text

Hartigan, P. M., J. D. Hamilton, and M. S. Simberkoff. “Early zidovudine and survival in HIV infection. The Veterans Affairs AIDS Cooperative Studies Group..” N Engl J Med 327, no. 11 (September 10, 1992): 814–15.

PMID
1501667
Scholars@Duke

Hamilton, J. D. “Late treatment of HIV infection--is it better?.” Infect Agents Dis 1, no. 3 (June 1992): 156–62.

PMID
1365540
Scholars@Duke

Simberkoff, M. S., P. M. Hartigan, and J. D. Hamilton. “Clinical trials: to terminate or not to terminate. The VA Cooperative Studies Group on AIDS..” Ann Intern Med 116, no. 8 (April 15, 1992): 699–700.

PMID
1546878
Scholars@Duke

Pages