John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • M.D., University of Toledo, 1974

Publications

Perfect, JR, Tenor, JL, Miao, Y, and Brennan, RG. "Trehalose pathway as an antifungal target." Virulence 8, no. 2 (February 2017): 143-149. (Review)

PMID
27248439
Full Text

Perfect, JR. "Reply to Argüelles." Virulence 8, no. 2 (February 2017): 239-. (letter)

PMID
27494147
Full Text

Chen, Y, and Perfect, JR. "Efficient, Cost-Effective, High-Throughput, Multilocus Sequencing Typing (MLST) Method, NGMLST, and the Analytical Software Program MLSTEZ." Methods in molecular biology (Clifton, N.J.) 1492 (January 2017): 197-202.

PMID
27822866
Full Text

Matzaraki, V, Gresnigt, MS, Jaeger, M, Ricaño-Ponce, I, Johnson, MD, Oosting, M, Franke, L, Withoff, S, Perfect, JR, Joosten, LAB, Kullberg, BJ, van de Veerdonk, FL, Jonkers, I, Li, Y, Wijmenga, C, Netea, MG, and Kumar, V. "An integrative genomics approach identifies novel pathways that influence candidaemia susceptibility." PloS one 12, no. 7 (January 2017): e0180824-.

PMID
28727728
Full Text

Messina, JA, Maziarz, EK, Spec, A, Kontoyiannis, DP, and Perfect, JR. "Disseminated cryptococcosis with brain involvement in patients with chronic lymphoid malignancies on ibrutinib." Open Forum Infectious Diseases 4, no. 1 (January 1, 2017).

Full Text

Chang, CC, and Perfect, JR. "Repeated therapeutic lumbar punctures in cryptococcal meningitis - necessity and/or opportunity?." Current opinion in infectious diseases 29, no. 6 (December 2016): 539-545.

PMID
27607912
Full Text

Yu, Y, Sabulski, MJ, Schell, WA, Pires, MM, Perfect, JR, and Regen, SL. "Simple Strategy for Taming Membrane-Disrupting Antibiotics." Bioconjugate chemistry 27, no. 12 (December 2016): 2850-2853.

PMID
27801580
Full Text

Shorman, M, Evans, D, Gibson, C, and Perfect, J. "Cases of disseminated cryptococcosis in intravenous drug abusers without HIV infection: A new risk factor?." Medical Mycology Case Reports 14 (December 2016): 17-19.

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Lortholary, O, Fernández-Ruiz, M, and Perfect, JR. "The current treatment landscape: other fungal diseases (cryptococcosis, fusariosis and mucormycosis)." The Journal of antimicrobial chemotherapy 71, no. suppl 2 (November 2016): ii31-ii36.

PMID
27880667
Full Text

Steenwyk, JL, Soghigian, JS, Perfect, JR, and Gibbons, JG. "Copy number variation contributes to cryptic genetic variation in outbreak lineages of Cryptococcus gattii from the North American Pacific Northwest." BMC genomics 17 (September 2, 2016): 700-.

PMID
27590805
Full Text

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