John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan at Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., University of Toledo, 1974

Publications

Cornely, Oliver A., Kathleen M. Mullane, Luis Ostrosky-Zeichner, Rochelle M. Maher, Rodney Croos-Dabrera, Qiaoyang Lu, Christopher Lademacher, et al. “Isavuconazole for treatment of rare invasive fungal diseases..” Mycoses 61, no. 8 (August 2018): 518–33. https://doi.org/10.1111/myc.12778.

PMID
29611246
Full Text

Ponzio, Vinicius, Luis Fernando Camargo, José Medina-Pestana, John Robert Perfect, and Arnaldo Lopes Colombo. “Outcomes of cryptococcosis in renal transplant recipients in a less-resourced health care system..” Transpl Infect Dis 20, no. 4 (August 2018). https://doi.org/10.1111/tid.12910.

PMID
29677399
Full Text

Shaw, Karen Joy, Wiley A. Schell, Jonathan Covel, Gisele Duboc, C. Giamberardino, Mili Kapoor, Molly Moloney, et al. “In Vitro and In Vivo Evaluation of APX001A/APX001 and Other Gwt1 Inhibitors against Cryptococcus..” Antimicrob Agents Chemother 62, no. 8 (August 2018). https://doi.org/10.1128/AAC.00523-18.

PMID
29891599
Full Text

Hussaini, Syed Mohammed Qasim, Deng Madut, Betty C. Tong, Elizabeth N. Pavlisko, Wiley A. Schell, John R. Perfect, and Nathan M. Thielman. “Pulmonary blastomycosis presenting as primary lung cancer..” Bmc Infect Dis 18, no. 1 (July 18, 2018). https://doi.org/10.1186/s12879-018-3244-0.

PMID
30021526
Full Text

Perfect, John R., Oliver A. Cornely, Markus Heep, Luis Ostrosky-Zeichner, Kathleen M. Mullane, Rochelle Maher, Rodney Croos-Dabrera, et al. “Isavuconazole treatment for rare fungal diseases and for invasive aspergillosis in patients with renal impairment: Challenges and lessons of the VITAL trial..” Mycoses 61, no. 7 (July 2018): 420–29. https://doi.org/10.1111/myc.12769.

PMID
29570857
Full Text

Marty, Francisco M., Oliver A. Cornely, Kathleen M. Mullane, Luis Ostrosky-Zeichner, Rochelle M. Maher, Rodney Croos-Dabrera, Qiaoyang Lu, et al. “Isavuconazole for treatment of invasive fungal diseases caused by more than one fungal species..” Mycoses 61, no. 7 (July 2018): 485–97. https://doi.org/10.1111/myc.12777.

PMID
29611227
Full Text

Maskarinec, Stacey A., Zehra Parlak, Qing Tu, Vrad Levering, Stefan Zauscher, Gabriel P. López, Vance G. Fowler, and John R. Perfect. “On-demand release of Candida albicans biofilms from urinary catheters by mechanical surface deformation..” Biofouling 34, no. 6 (July 2018): 595–604. https://doi.org/10.1080/08927014.2018.1474461.

PMID
29897277
Full Text

Mourad, A., melissa Johnson, and john Perfect. “Predictors of Mortality in Patients Receiving Voriconazole.” Atlanta, GA: American Society of Microbiology - Microbe, 2018.

Scholars@Duke

Garcia-Santamarina, Sarela, Richard A. Festa, Aaron D. Smith, Chen-Hsin Yu, Corinna Probst, Chen Ding, Christina M. Homer, et al. “Genome-wide analysis of the regulation of Cu metabolism in Cryptococcus neoformans..” Mol Microbiol 108, no. 5 (June 2018): 473–94. https://doi.org/10.1111/mmi.13960.

PMID
29608794
Full Text

Pages