John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan at Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., University of Toledo, 1974

Publications

Miao, Y, Tenor, JL, Toffaletti, DL, Maskarinec, SA, Liu, J, Lee, RE, Perfect, JR, and Brennan, RG. "Structural and In Vivo Studies on Trehalose-6-Phosphate Synthase from Pathogenic Fungi Provide Insights into Its Catalytic Mechanism, Biological Necessity, and Potential for Novel Antifungal Drug Design." mBio 8, no. 4 (July 25, 2017).

PMID
28743811
Full Text

Desjardins, CA, Giamberardino, C, Sykes, SM, Yu, C-H, Tenor, JL, Chen, Y, Yang, T, Jones, AM, Sun, S, Haverkamp, MR, Heitman, J, Litvintseva, AP, Perfect, JR, and Cuomo, CA. "Population genomics and the evolution of virulence in the fungal pathogen Cryptococcus neoformans." Genome research 27, no. 7 (July 2017): 1207-1219.

PMID
28611159
Full Text

Thammahong, A, Puttikamonkul, S, Perfect, JR, Brennan, RG, and Cramer, RA. "Central Role of the Trehalose Biosynthesis Pathway in the Pathogenesis of Human Fungal Infections: Opportunities and Challenges for Therapeutic Development." Microbiology and molecular biology reviews : MMBR 81, no. 2 (June 2017). (Review)

PMID
28298477
Full Text

Lestner, J, McEntee, L, Johnson, A, Livermore, J, Whalley, S, Schwartz, J, Perfect, JR, Harrison, T, and Hope, W. "Experimental Models of Short Courses of Liposomal Amphotericin B for Induction Therapy for Cryptococcal Meningitis." Antimicrobial agents and chemotherapy 61, no. 6 (June 2017).

PMID
28320715
Full Text

Köhler, JR, Hube, B, Puccia, R, Casadevall, A, and Perfect, JR. "Fungi that Infect Humans." Microbiology spectrum 5, no. 3 (June 2017). (Review)

PMID
28597822
Full Text

Chen, Y, Farrer, RA, Giamberardino, C, Sakthikumar, S, Jones, A, Yang, T, Tenor, JL, Wagih, O, Van Wyk, M, Govender, NP, Mitchell, TG, Litvintseva, AP, Cuomo, CA, and Perfect, JR. "Microevolution of Serial Clinical Isolates of Cryptococcus neoformans var. grubii and C. gattii." mBio 8, no. 2 (March 7, 2017).

PMID
28270580
Full Text

Oehlers, SH, Cronan, MR, Beerman, RW, Johnson, MG, Huang, J, Kontos, CD, Stout, JE, and Tobin, DM. "Infection-Induced Vascular Permeability Aids Mycobacterial Growth." The Journal of infectious diseases 215, no. 5 (March 2017): 813-817.

PMID
27496976
Full Text

Perfect, JR, Tenor, JL, Miao, Y, and Brennan, RG. "Trehalose pathway as an antifungal target." Virulence 8, no. 2 (February 2017): 143-149. (Review)

PMID
27248439
Full Text

Perfect, JR. "Reply to Argüelles." Virulence 8, no. 2 (February 2017): 239-. (Letter)

PMID
27494147
Full Text

Farber, SH, Murphy, KR, Suryadevara, CM, Babu, R, Yang, S, Feng, L, Xie, J, Perfect, JR, and Lad, SP. "Comparing outcomes of early, late, and non-surgical management of intraspinal abscess." Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia 36 (February 2017): 64-71.

PMID
27836393
Full Text

Pages