John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan at Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., University of Toledo, 1974

Publications

Smilnak, GJ, Charalambous, LT, Cutshaw, D, Premji, AM, Giamberardino, CD, Ballard, CG, Bartuska, AP, Ejikeme, TU, Sheng, H, Verbick, LZ, Hedstrom, BA, Pagadala, PC, McCabe, AR, Perfect, JR, and Lad, SP. "Novel Treatment of Cryptococcal Meningitis via NeurapheresisTM Therapy." The Journal of Infectious Diseases (May 17, 2018).

PMID
29788431
Full Text

Ponzio, V, Camargo, LF, Medina-Pestana, J, Perfect, JR, and Colombo, AL. "Outcomes of cryptococcosis in renal transplant recipients in a less-resourced health care system." Transplant Infectious Disease : an Official Journal of the Transplantation Society (April 20, 2018): e12910-null.

PMID
29677399
Full Text

Cornely, OA, Mullane, KM, Ostrosky-Zeichner, L, Maher, RM, Croos-Dabrera, R, Lu, Q, Lademacher, C, Perfect, JR, Oren, I, Schmitt-Hoffmann, A-H, Giladi, M, Marty, FM, and Rahav, G. "Isavuconazole for treatment of rare invasive fungal diseases." Mycoses (April 3, 2018).

PMID
29611246
Full Text

Vasquez, A, Zavasky, D, Chow, NA, Gade, L, Zlatanic, E, Elkind, S, Litvintseva, AP, Pappas, PG, Perfect, JR, Revankar, S, Lockhart, SR, Chiller, T, Ackelsberg, J, and Vallabhaneni, S. "Management of an Outbreak of Exophiala dermatitidis Bloodstream Infections at an Outpatient Oncology Clinic." Clinical infectious diseases : an official publication of the Infectious Diseases Society of America 66, no. 6 (March 2018): 959-962.

PMID
29121235
Full Text

Saelens, JW, Lau-Bonilla, D, Moller, A, Xet-Mull, AM, Medina, N, Guzmán, B, Calderón, M, Herrera, R, Stout, JE, Arathoon, E, Samayoa, B, and Tobin, DM. "Annotated Genome Sequences of 16 Lineage 4 Mycobacterium tuberculosis Strains from Guatemala." Genome Announcements 6, no. 7 (February 15, 2018).

PMID
29449380
Full Text

Charalambous, LT, Premji, A, Tybout, C, Hunt, A, Cutshaw, D, Elsamadicy, AA, Yang, S, Xie, J, Giamberardino, C, Pagadala, P, Perfect, JR, and Lad, SP. "Prevalence, healthcare resource utilization and overall burden of fungal meningitis in the United States." Journal of Medical Microbiology 67, no. 2 (February 2018): 215-227.

PMID
29244019
Full Text

Mourad, A, and Perfect, JR. "The war on cryptococcosis: A Review of the antifungal arsenal." Memorias Do Instituto Oswaldo Cruz 113, no. 7 (January 2018): e170391-null. (Review)

PMID
29513785
Full Text

Mourad, A, and Perfect, JR. "Tolerability profile of the current antifungal armoury." The Journal of Antimicrobial Chemotherapy 73, no. suppl_1 (January 2018): i26-i32.

PMID
29304209
Full Text

Wilkinson, RJ, Rohlwink, U, Misra, UK, van Crevel, R, Mai, NTH, Dooley, KE, Caws, M, Figaji, A, Savic, R, Solomons, R, and Thwaites, GE. "Tuberculous meningitis." Nature Reviews Neurology 13, no. 10 (September 8, 2017): 581-598.

Full Text

Perfect, JR. "The antifungal pipeline: a reality check." Nature Reviews. Drug Discovery 16, no. 9 (September 2017): 603-616. (Review)

PMID
28496146
Full Text

Pages