John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan at Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., University of Toledo, 1974

Publications

Chang, Christina C., and John R. Perfect. “Repeated therapeutic lumbar punctures in cryptococcal meningitis - necessity and/or opportunity?.” Curr Opin Infect Dis 29, no. 6 (December 2016): 539–45. https://doi.org/10.1097/QCO.0000000000000315.

PMID
27607912
Full Text

Lortholary, Olivier, Mario Fernández-Ruiz, and John R. Perfect. “The current treatment landscape: other fungal diseases (cryptococcosis, fusariosis and mucormycosis)..” J Antimicrob Chemother 71, no. suppl 2 (November 2016): ii31–36. https://doi.org/10.1093/jac/dkw394.

PMID
27880667
Full Text

Schneeweiss, Sebastian, Peggy L. Carver, Kausik Datta, Alicia Galar, Melissa D. Johnson, Matthew G. Johnson, Francisco M. Marty, et al. “Short-term risk of liver and renal injury in hospitalized patients using micafungin: a multicentre cohort study..” J Antimicrob Chemother 71, no. 10 (October 2016): 2938–44. https://doi.org/10.1093/jac/dkw225.

PMID
27407049
Full Text

Cournoyer, Justin M., Aven P. Garms, Kimberly N. Thiessen, Margaret T. Bowers, Melissa D. Johnson, and Michael V. Relf. “Cardiovascular Disease and HIV: Pathophysiology, Treatment Considerations, and Nursing Implications..” Crit Care Nurse 36, no. 5 (October 2016): 37–46. https://doi.org/10.4037/ccn2016839.

PMID
27694356
Full Text

Steenwyk, Jacob L., John S. Soghigian, John R. Perfect, and John G. Gibbons. “Copy number variation contributes to cryptic genetic variation in outbreak lineages of Cryptococcus gattii from the North American Pacific Northwest..” Bmc Genomics 17 (September 2, 2016). https://doi.org/10.1186/s12864-016-3044-0.

PMID
27590805
Full Text

Thompson, George R., Adrian Rendon, Rodrigo Ribeiro Dos Santos, Flavio Queiroz-Telles, Luis Ostrosky-Zeichner, Nkechi Azie, Rochelle Maher, et al. “Isavuconazole Treatment of Cryptococcosis and Dimorphic Mycoses..” Clin Infect Dis 63, no. 3 (August 1, 2016): 356–62. https://doi.org/10.1093/cid/ciw305.

PMID
27169478
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Marty, Francisco M., Luis Ostrosky-Zeichner, Oliver A. Cornely, Kathleen M. Mullane, John R. Perfect, George R. Thompson, George J. Alangaden, et al. “Isavuconazole treatment for mucormycosis: a single-arm open-label trial and case-control analysis..” Lancet Infect Dis 16, no. 7 (July 2016): 828–37. https://doi.org/10.1016/S1473-3099(16)00071-2.

PMID
26969258
Full Text

Miao, Yi, Jennifer L. Tenor, Dena L. Toffaletti, Erica J. Washington, Jiuyu Liu, William R. Shadrick, Maria A. Schumacher, Richard E. Lee, John R. Perfect, and Richard G. Brennan. “Structures of trehalose-6-phosphate phosphatase from pathogenic fungi reveal the mechanisms of substrate recognition and catalysis..” Proc Natl Acad Sci U S A 113, no. 26 (June 28, 2016): 7148–53. https://doi.org/10.1073/pnas.1601774113.

PMID
27307435
Full Text

Perfect, John R. “Editorial Commentary: Life-saving Antimicrobial Drugs: What Are We Doing to Pricing and Availability?.” Clin Infect Dis 62, no. 12 (June 15, 2016): 1569–70. https://doi.org/10.1093/cid/ciw153.

PMID
27009252
Full Text

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