John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan at Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., University of Toledo, 1974

Publications

Cournoyer, JM, Garms, AP, Thiessen, KN, Bowers, MT, Johnson, MD, and Relf, MV. "Cardiovascular Disease and HIV: Pathophysiology, Treatment Considerations, and Nursing Implications." Critical care nurse 36, no. 5 (October 2016): 37-46. (Review)

PMID
27694356
Full Text

Cronan, MR, Beerman, RW, Rosenberg, AF, Saelens, JW, Johnson, MG, Oehlers, SH, Sisk, DM, Jurcic Smith, KL, Medvitz, NA, Miller, SE, Trinh, LA, Fraser, SE, Madden, JF, Turner, J, Stout, JE, Lee, S, and Tobin, DM. "Macrophage Epithelial Reprogramming Underlies Mycobacterial Granuloma Formation and Promotes Infection." Immunity 45, no. 4 (October 2016): 861-876.

PMID
27760340
Full Text

Steenwyk, JL, Soghigian, JS, Perfect, JR, and Gibbons, JG. "Copy number variation contributes to cryptic genetic variation in outbreak lineages of Cryptococcus gattii from the North American Pacific Northwest." Bmc Genomics 17 (September 2, 2016): 700-null.

PMID
27590805
Full Text

Matty, MA, Beerman, RW, and Tobin, DM. "Drug-Inducible, Cell-Specific Manipulation of Intracellular Calcium in Zebrafish Through Mammalian TRPV1 Expression." Zebrafish 13, no. 4 (August 2016): 374-375.

PMID
27058231
Full Text

Thompson, GR, Rendon, A, Ribeiro Dos Santos, R, Queiroz-Telles, F, Ostrosky-Zeichner, L, Azie, N, Maher, R, Lee, M, Kovanda, L, Engelhardt, M, Vazquez, JA, Cornely, OA, and Perfect, JR. "Isavuconazole Treatment of Cryptococcosis and Dimorphic Mycoses." Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America 63, no. 3 (August 2016): 356-362.

PMID
27169478
Full Text

Marty, FM, Ostrosky-Zeichner, L, Cornely, OA, Mullane, KM, Perfect, JR, Thompson, GR, Alangaden, GJ, Brown, JM, Fredricks, DN, Heinz, WJ, Herbrecht, R, Klimko, N, Klyasova, G, Maertens, JA, Melinkeri, SR, Oren, I, Pappas, PG, Ráčil, Z, Rahav, G, Santos, R, Schwartz, S, Vehreschild, JJ, Young, J-AH, Chetchotisakd, P, Jaruratanasirikul, S, Kanj, SS, Engelhardt, M, Kaufhold, A, Ito, M, Lee, M, Sasse, C, Maher, RM, Zeiher, B, Vehreschild, MJGT, and VITAL and FungiScope Mucormycosis Investigators, . "Isavuconazole treatment for mucormycosis: a single-arm open-label trial and case-control analysis." The Lancet. Infectious Diseases 16, no. 7 (July 2016): 828-837.

PMID
26969258
Full Text

Miao, Y, Tenor, JL, Toffaletti, DL, Washington, EJ, Liu, J, Shadrick, WR, Schumacher, MA, Lee, RE, Perfect, JR, and Brennan, RG. "Structures of trehalose-6-phosphate phosphatase from pathogenic fungi reveal the mechanisms of substrate recognition and catalysis." Proceedings of the National Academy of Sciences of the United States of America 113, no. 26 (June 15, 2016): 7148-7153.

PMID
27307435
Full Text

Maskarinec, SA, Johnson, MD, and Perfect, JR. "Genetic Susceptibility to Fungal Infections: What is in the Genes?." Current Clinical Microbiology Reports 3, no. 2 (June 2016): 81-91.

PMID
27547700
Full Text

Homer, CM, Summers, DK, Goranov, AI, Clarke, SC, Wiesner, DL, Diedrich, JK, Moresco, JJ, Toffaletti, D, Upadhya, R, Caradonna, I, Petnic, S, Pessino, V, Cuomo, CA, Lodge, JK, Perfect, J, Yates, JR, Nielsen, K, Craik, CS, and Madhani, HD. "Intracellular Action of a Secreted Peptide Required for Fungal Virulence." Cell host & microbe 19, no. 6 (June 2016): 849-864.

PMID
27212659
Full Text

Perfect, JR. ""Is there an emerging need for new antifungals?"." Expert Opinion on Emerging Drugs 21, no. 2 (June 2016): 129-131.

PMID
26883732
Full Text

Pages