John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan at Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., University of Toledo, 1974

Publications

Roth, S, Bergmann, H, Jaeger, M, Yeroslaviz, A, Neumann, K, Koenig, P-A, Prazeres da Costa, C, Vanes, L, Kumar, V, Johnson, M, Menacho-Márquez, M, Habermann, B, Tybulewicz, VL, Netea, M, Bustelo, XR, and Ruland, J. "Vav Proteins Are Key Regulators of Card9 Signaling for Innate Antifungal Immunity." Cell Reports 17, no. 10 (December 2016): 2572-2583.

PMID
27926862
Full Text

Lortholary, O, Fernández-Ruiz, M, and Perfect, JR. "The current treatment landscape: other fungal diseases (cryptococcosis, fusariosis and mucormycosis)." The Journal of antimicrobial chemotherapy 71, no. suppl 2 (November 2016): ii31-ii36.

PMID
27880667
Full Text

McClean, CM, and Tobin, DM. "Macrophage form, function, and phenotype in mycobacterial infection: lessons from tuberculosis and other diseases." Pathogens and Disease 74, no. 7 (October 2016). (Review)

PMID
27402783
Full Text

Schneeweiss, S, Carver, PL, Datta, K, Galar, A, Johnson, MD, Johnson, MG, Marty, FM, Nagel, J, Najdzinowicz, M, Saul, M, Shoham, S, Silveira, FP, Varughese, CA, Wilck, M, Weatherby, L, Auton, T, and Walker, AM. "Short-term risk of liver and renal injury in hospitalized patients using micafungin: a multicentre cohort study." The Journal of antimicrobial chemotherapy 71, no. 10 (October 2016): 2938-2944.

PMID
27407049
Full Text

Cournoyer, JM, Garms, AP, Thiessen, KN, Bowers, MT, Johnson, MD, and Relf, MV. "Cardiovascular Disease and HIV: Pathophysiology, Treatment Considerations, and Nursing Implications." Critical care nurse 36, no. 5 (October 2016): 37-46. (Review)

PMID
27694356
Full Text

Cronan, MR, Beerman, RW, Rosenberg, AF, Saelens, JW, Johnson, MG, Oehlers, SH, Sisk, DM, Jurcic Smith, KL, Medvitz, NA, Miller, SE, Trinh, LA, Fraser, SE, Madden, JF, Turner, J, Stout, JE, Lee, S, and Tobin, DM. "Macrophage Epithelial Reprogramming Underlies Mycobacterial Granuloma Formation and Promotes Infection." Immunity 45, no. 4 (October 2016): 861-876.

PMID
27760340
Full Text

Steenwyk, JL, Soghigian, JS, Perfect, JR, and Gibbons, JG. "Copy number variation contributes to cryptic genetic variation in outbreak lineages of Cryptococcus gattii from the North American Pacific Northwest." Bmc Genomics 17 (September 2, 2016): 700-null.

PMID
27590805
Full Text

Matty, MA, Beerman, RW, and Tobin, DM. "Drug-Inducible, Cell-Specific Manipulation of Intracellular Calcium in Zebrafish Through Mammalian TRPV1 Expression." Zebrafish 13, no. 4 (August 2016): 374-375.

PMID
27058231
Full Text

Thompson, GR, Rendon, A, Ribeiro Dos Santos, R, Queiroz-Telles, F, Ostrosky-Zeichner, L, Azie, N, Maher, R, Lee, M, Kovanda, L, Engelhardt, M, Vazquez, JA, Cornely, OA, and Perfect, JR. "Isavuconazole Treatment of Cryptococcosis and Dimorphic Mycoses." Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America 63, no. 3 (August 2016): 356-362.

PMID
27169478
Full Text

Marty, FM, Ostrosky-Zeichner, L, Cornely, OA, Mullane, KM, Perfect, JR, Thompson, GR, Alangaden, GJ, Brown, JM, Fredricks, DN, Heinz, WJ, Herbrecht, R, Klimko, N, Klyasova, G, Maertens, JA, Melinkeri, SR, Oren, I, Pappas, PG, Ráčil, Z, Rahav, G, Santos, R, Schwartz, S, Vehreschild, JJ, Young, J-AH, Chetchotisakd, P, Jaruratanasirikul, S, Kanj, SS, Engelhardt, M, Kaufhold, A, Ito, M, Lee, M, Sasse, C, Maher, RM, Zeiher, B, Vehreschild, MJGT, and VITAL and FungiScope Mucormycosis Investigators, . "Isavuconazole treatment for mucormycosis: a single-arm open-label trial and case-control analysis." The Lancet. Infectious Diseases 16, no. 7 (July 2016): 828-837.

PMID
26969258
Full Text

Pages