John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • M.D., University of Toledo, 1974

Publications

Kumar, V, Cheng, S-C, Johnson, MD, Smeekens, SP, Wojtowicz, A, Giamarellos-Bourboulis, E, Karjalainen, J, Franke, L, Withoff, S, Plantinga, TS, van de Veerdonk, FL, van der Meer, JWM, Joosten, LAB, Bochud, P-Y, Marchetti, O, Perfect, JR, Xavier, R, Kullberg, BJ, Wijmenga, C, and Netea, MG. "Immunochip SNP array identifies novel genetic variants conferring susceptibility to candidaemia." Nature communications 5 (September 8, 2014): 4675-.

PMID
25197941
Full Text

Perfect, JR. "Cryptococcosis (Cryptococcus neoformans and Cryptococcus gattii)." In Mandell, Douglas, and Bennett's Principles and Practice of Infectious Diseases, 2934-2948. August 28, 2014.

Full Text

Jarvis, JN, Bicanic, T, Loyse, A, Meintjes, G, Hogan, L, Roberts, CH, Shoham, S, Perfect, JR, Govender, NP, and Harrison, TS. "Very low levels of 25-hydroxyvitamin D are not associated with immunologic changes or clinical outcome in South African patients with HIV-associated cryptococcal meningitis." Clinical infectious diseases : an official publication of the Infectious Diseases Society of America 59, no. 4 (August 2014): 493-500.

PMID
24825871
Full Text

Janout, V, Bienvenu, C, Schell, W, Perfect, JR, and Regen, SL. "Molecular umbrella-amphotericin B conjugates." Bioconjugate chemistry 25, no. 8 (August 2014): 1408-1411.

PMID
25068916
Full Text

Kung, H-C, Johnson, MD, Drew, RH, Saha-Chaudhuri, P, and Perfect, JR. "Clinical effectiveness of posaconazole versus fluconazole as antifungal prophylaxis in hematology-oncology patients: a retrospective cohort study." Cancer medicine 3, no. 3 (June 2014): 667-673.

PMID
24644249
Full Text

Rosentul, DC, Plantinga, TS, Farcas, M, Oosting, M, Hamza, OJM, Scott, WK, Alexander, BD, Yang, JC, Laird, GM, Joosten, LAB, van der Meer, JWM, Perfect, JR, Kullberg, B-J, van der Ven, AJAM, Johnson, MD, and Netea, MG. "Role of autophagy genetic variants for the risk of Candida infections." Medical mycology 52, no. 4 (May 2014): 333-341.

PMID
24713404
Full Text

Perfect, JR. "Cryptococcosis: a model for the understanding of infectious diseases." The Journal of clinical investigation 124, no. 5 (May 2014): 1893-1895.

PMID
24743152
Full Text

Smeekens, SP, Malireddi, RK, Plantinga, TS, Buffen, K, Oosting, M, Joosten, LAB, Kullberg, BJ, Perfect, JR, Scott, WK, van de Veerdonk, FL, Xavier, RJ, van de Vosse, E, Kanneganti, T-D, Johnson, MD, and Netea, MG. "Autophagy is redundant for the host defense against systemic Candida albicans infections." Eur J Clin Microbiol Infect Dis 33, no. 5 (May 2014): 711-722.

PMID
24202731
Full Text

Horan, JL, and Perfect, JR. "Fungal meningitis." In Infections of the Central Nervous System: Fourth Edition. April 21, 2014.

Scholars@Duke

Janbon, G, Ormerod, KL, Paulet, D, Byrnes, EJ, Yadav, V, Chatterjee, G, Mullapudi, N, Hon, C-C, Billmyre, RB, Brunel, F, Bahn, Y-S, Chen, W, Chen, Y, Chow, EWL, Coppée, J-Y, Floyd-Averette, A, Gaillardin, C, Gerik, KJ, Goldberg, J, Gonzalez-Hilarion, S, Gujja, S, Hamlin, JL, Hsueh, Y-P, Ianiri, G, Jones, S, Kodira, CD, Kozubowski, L, Lam, W, Marra, M, Mesner, LD, Mieczkowski, PA, Moyrand, F, Nielsen, K, Proux, C, Rossignol, T, Schein, JE, Sun, S, Wollschlaeger, C, Wood, IA, and Zeng, Q et al. "Analysis of the genome and transcriptome of Cryptococcus neoformans var. grubii reveals complex RNA expression and microevolution leading to virulence attenuation." PLoS genetics 10, no. 4 (April 17, 2014): e1004261-.

PMID
24743168
Full Text

Pages