John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan at Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., University of Toledo, 1974


Toffaletti, D. L., T. H. Rude, S. A. Johnston, D. T. Durack, and J. R. Perfect. “Gene transfer in Cryptococcus neoformans by use of biolistic delivery of DNA..” J Bacteriol 175, no. 5 (March 1993): 1405–11.

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Cox, G. M., and J. R. Perfect. “Fungal infections.” Current Opinion in Infectious Diseases 6, no. 3 (January 1, 1993): 422–26.


PERFECT, J. R. “Uptake of itraconazole by alveolar macrophages.” Antimicrob Agents Chemother 37 (1993): 903–4.


Perfect, J. R., T. H. Rude, L. M. Penning, and S. A. Johnson. “Cloning the Cryptococcus neoformans TRP1 gene by complementation in Saccharomyces cerevisiae..” Gene 122, no. 1 (December 1, 1992): 213–17.

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Perfect, J. R., M. E. Klotman, C. C. Gilbert, D. D. Crawford, G. L. Rosner, K. A. Wright, and W. P. Peters. “Prophylactic intravenous amphotericin B in neutropenic autologous bone marrow transplant recipients..” J Infect Dis 165, no. 5 (May 1992): 891–97.

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