John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan at Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., University of Toledo, 1974

Publications

Denning, D. W., J. Y. Lee, J. S. Hostetler, P. Pappas, C. A. Kauffman, D. H. Dewsnup, J. N. Galgiani, et al. “Erratum: NIAID mycoses study group multicenter trial of oral itraconazole therapy for invasive aspergillosis (American Journal of Medicine (August 1994) 97 (135-144)).” American Journal of Medicine 97, no. 5 (January 1, 1994). https://doi.org/10.1016/0002-9343(94)90341-7.

Full Text

Perfect, J. R., N. Ketabchi, G. M. Cox, C. W. Ingram, and C. L. Beiser. “Karyotyping of Cryptococcus neoformans as an epidemiological tool..” J Clin Microbiol 31, no. 12 (December 1993): 3305–9.

PMID
8308124
Scholars@Duke

Ingram, C. W., H. B. Haywood, V. M. Morris, R. L. Allen, and J. R. Perfect. “Cryptococcal ventricular-peritoneal shunt infection: clinical and epidemiological evaluation of two closely associated cases..” Infect Control Hosp Epidemiol 14, no. 12 (December 1993): 719–22.

PMID
8132998
Scholars@Duke

Cameron, M. L., W. A. Schell, S. Bruch, J. A. Bartlett, H. A. Waskin, and J. R. Perfect. “Correlation of in vitro fluconazole resistance of Candida isolates in relation to therapy and symptoms of individuals seropositive for human immunodeficiency virus type 1..” Antimicrob Agents Chemother 37, no. 11 (November 1993): 2449–53. https://doi.org/10.1128/aac.37.11.2449.

PMID
8285632
Full Text

Perfect, J. R., D. L. Toffaletti, and T. H. Rude. “The gene encoding phosphoribosylaminoimidazole carboxylase (ADE2) is essential for growth of Cryptococcus neoformans in cerebrospinal fluid..” Infect Immun 61, no. 10 (October 1993): 4446–51.

PMID
8406836
Scholars@Duke

Sharkey-Mathis, P. K., C. A. Kauffman, J. R. Graybill, D. A. Stevens, J. S. Hostetler, G. Cloud, and W. E. Dismukes. “Treatment of sporotrichosis with itraconazole. NIAID Mycoses Study Group..” Am J Med 95, no. 3 (September 1993): 279–85.

PMID
8396321
Scholars@Duke

Perfect, J. R., D. V. Savani, and D. T. Durack. “Uptake of itraconazole by alveolar macrophages..” Antimicrob Agents Chemother 37, no. 4 (April 1993): 903–4. https://doi.org/10.1128/aac.37.4.903.

PMID
8388205
Full Text

Perfect, J. R. “Antifungal prophylaxis: to prevent or not..” Am J Med 94, no. 3 (March 1993): 233–34.

PMID
8452146
Scholars@Duke

Toffaletti, D. L., T. H. Rude, S. A. Johnston, D. T. Durack, and J. R. Perfect. “Gene transfer in Cryptococcus neoformans by use of biolistic delivery of DNA..” J Bacteriol 175, no. 5 (March 1993): 1405–11. https://doi.org/10.1128/jb.175.5.1405-1411.1993.

PMID
8444802
Full Text

Pages