John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan at Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., University of Toledo, 1974

Publications

Granger, D. L., J. R. Perfect, and D. T. Durack. “Macrophage-mediated fungistasis: requirement for a macromolecular component in serum..” J Immunol 137, no. 2 (July 15, 1986): 693–701.

PMID
3522734
Scholars@Duke

GRANGER, D. L., J. R. PERFECT, and D. T. DURACK. “INHIBITION OF FUNGAL CELL REPLICATION BY MACROPHAGES - REQUIREMENT FOR SERUM FACTOR(S).” In Zentralblatt Fur Bakteriologie Mikrobiologie Und Hygiene Series a Medical Microbiology Infectious Diseases Virology Parasitology, 262:58–58. GUSTAV FISCHER VERLAG, 1986.

Scholars@Duke

PERFECT, J. R., and D. T. DURACK. “THE ANTIFUNGAL ACTIVITY OF THE TRIANGLE UK-49,858 IN THE TREATMENT OF EXPERIMENTAL CANDIDA PYELONEPHRITIS AND CRYPTOCOCCAL MENINGITIS.” In Zentralblatt Fur Bakteriologie Mikrobiologie Und Hygiene Series a Medical Microbiology Infectious Diseases Virology Parasitology, 262:153–54. GUSTAV FISCHER VERLAG, 1986.

Scholars@Duke

GRANGER, D. L., J. R. PERFECT, and D. T. DURACK. “ROLE OF IRON AND HEME BINDING SERUM-PROTEINS IN MACROPHAGE FUNGISTASIS.” In Clinical Research, 34:A658–A658. SLACK INC, 1986.

Scholars@Duke

PERFECT, J. R., M. M. HOBBS, D. L. GRANGER, and D. T. DURACK. “DYNAMICS OF CEREBROSPINAL-FLUID MACROPHAGE ACTIVATION IN EXPERIMENTAL CRYPTOCOCCAL MENINGITIS.” In Clinical Research, 34:A678–A678. SLACK INC, 1986.

Scholars@Duke

Perfect, J. R., D. V. Savani, and D. T. Durack. “Comparison of itraconazole and fluconazole in treatment of cryptococcal meningitis and candida pyelonephritis in rabbits..” Antimicrob Agents Chemother 29, no. 4 (April 1986): 579–83. https://doi.org/10.1128/aac.29.4.579.

PMID
3010846
Full Text

Granger, D. L., J. R. Perfect, and D. T. Durack. “Macrophage-mediated fungistasis in vitro: requirements for intracellular and extracellular cytotoxicity..” J Immunol 136, no. 2 (January 1986): 672–80.

PMID
3510251
Scholars@Duke

PERFECT, J. R. “Comparison of itraconazole and flucanazole in the treatment of cryptococcal meningitis and Candida pyelonephritis in rabbits.” Antimicr Agents Chemoth 29 (1986): 579–83.

Scholars@Duke

Perfect, J. R., and D. T. Durack. “Comparison of amphotericin B and N-D-ornithyl amphotericin B methyl ester in experimental cryptococcal meningitis and Candida albicans endocarditis with pyelonephritis..” Antimicrob Agents Chemother 28, no. 6 (December 1985): 751–55. https://doi.org/10.1128/aac.28.6.751.

PMID
4083860
Full Text

Perfect, J. R., and D. T. Durack. “Effects of cyclosporine in experimental cryptococcal meningitis..” Infect Immun 50, no. 1 (October 1985): 22–26.

PMID
3899932
Scholars@Duke

Pages