John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan at Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., University of Toledo, 1974

Publications

Drew, R. H., J. R. Perfect, and H. A. Gallis. “Use of fluconazole in a patient with documented malabsorption of ketoconazole..” Clin Pharm 7, no. 8 (August 1988): 622–23.

PMID
2844468
Scholars@Duke

Perfect, J. R., M. M. Hobbs, D. L. Granger, and D. T. Durack. “Cerebrospinal fluid macrophage response to experimental cryptococcal meningitis: relationship between in vivo and in vitro measurements of cytotoxicity..” Infect Immun 56, no. 4 (April 1988): 849–54.

PMID
3346075
Scholars@Duke

Granger, D. L., J. B. Hibbs, J. R. Perfect, and D. T. Durack. “Specific amino acid (L-arginine) requirement for the microbiostatic activity of murine macrophages..” J Clin Invest 81, no. 4 (April 1988): 1129–36. https://doi.org/10.1172/JCI113427.

PMID
3280600
Full Text

Hobbs, M., J. Perfect, and D. Durack. “Evaluation of in vitro antifungal activity of LY121019..” Eur J Clin Microbiol Infect Dis 7, no. 1 (February 1988): 77–80.

PMID
3132385
Scholars@Duke

Perfect, J. R. “Mycobacterium avium-intracellulare complex infections in the acquired immunodeficiency syndrome..” J Electron Microsc Tech 8, no. 1 (January 1988): 105–13. https://doi.org/10.1002/jemt.1060080107.

PMID
3073192
Full Text

Kim, J. H., and J. R. Perfect. “To help the sick: an historical and ethical essay concerning the refusal to care for patients with AIDS..” Am J Med 84, no. 1 (January 1988): 135–38.

PMID
3276180
Scholars@Duke

Perfect, J. R., D. L. Granger, and D. T. Durack. “Effects of antifungal agents and gamma interferon on macrophage cytotoxicity for fungi and tumor cells..” J Infect Dis 156, no. 2 (August 1987): 316–23. https://doi.org/10.1093/infdis/156.2.316.

PMID
3110308
Full Text

Gross, M. H., W. W. Pickard, and J. R. Perfect. “Retrospective review of amphotericin B use in a tertiary-care medical center..” Am J Hosp Pharm 44, no. 6 (June 1987): 1353–57.

PMID
3618612
Scholars@Duke

Pages