John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan at Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., University of Toledo, 1974

Publications

Kim, JH, and Perfect, JR. "To help the sick: an historical and ethical essay concerning the refusal to care for patients with AIDS." Am J Med 84, no. 1 (January 1988): 135-138.

PMID
3276180
Scholars@Duke

Hobbs, M, Perfect, J, and Durack, D. "Evaluation of in vitro antifungal activity of LY121019." European Journal of Clinical Microbiology & Infectious Diseases 7, no. 1 (1988): 77-80.

PMID
3132385
Full Text

Perfect, JR, Granger, DL, and Durack, DT. "Effects of antifungal agents and gamma interferon on macrophage cytotoxicity for fungi and tumor cells." J Infect Dis 156, no. 2 (August 1987): 316-323.

PMID
3110308
Scholars@Duke

Gross, MH, Pickard, WW, and Perfect, JR. "Retrospective review of amphotericin B use in a tertiary-care medical center." Am J Hosp Pharm 44, no. 6 (June 1987): 1353-1357.

PMID
3618612
Scholars@Duke

Lallinger, GJ, Reiner, SL, Cooke, DW, Toffaletti, DL, Perfect, JR, Granger, DL, and Durack, DT. "Efficacy of immune therapy in early experimental Naegleria fowleri meningitis." Infect Immun 55, no. 5 (May 1987): 1289-1293.

PMID
3570464
Scholars@Duke

GRANGER, DL, HIBBS, JB, PERFECT, JR, and DURACK, DT. "ARGININE-DEPENDENT MICROBIOSTATIC PATHWAY OF MACROPHAGES PRODUCING NO-2/NO-3." April 1987.

Scholars@Duke

Savani, DV, Perfect, JR, Cobo, LM, and Durack, DT. "Penetration of new azole compounds into the eye and efficacy in experimental Candida endophthalmitis." Antimicrobial Agents and Chemotherapy 31, no. 1 (January 1987): 6-10.

PMID
3032091
Full Text

GRANGER, DL, PERFECT, JR, and DURACK, DT. "REQUIREMENTS FOR THE EXPRESSION OF THE MICROBIOSTATIC ACTION OF MACROPHAGES." 1987.

Scholars@Duke

Granger, DL, Perfect, JR, and Durack, DT. "Macrophage-mediated fungistasis: requirement for a macromolecular component in serum." J Immunol 137, no. 2 (July 15, 1986): 693-701.

PMID
3522734
Scholars@Duke

GRANGER, DL, PERFECT, JR, and DURACK, DT. "INHIBITION OF FUNGAL CELL REPLICATION BY MACROPHAGES - REQUIREMENT FOR SERUM FACTOR(S)." July 1986.

Scholars@Duke

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