John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • M.D., University of Toledo, 1974

Publications

Drew, RH, Townsend, ML, Pound, MW, Johnson, SW, and Perfect, JR. "Recent advances in the treatment of life-threatening, invasive fungal infections." Expert Opin Pharmacother 14, no. 17 (December 2013): 2361-2374. (Review)

PMID
24050675
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Lionakis, MS, Swamydas, M, Fischer, BG, Plantinga, TS, Johnson, MD, Jaeger, M, Green, NM, Masedunskas, A, Weigert, R, Mikelis, C, Wan, W, Lee, C-CR, Lim, JK, Rivollier, A, Yang, JC, Laird, GM, Wheeler, RT, Alexander, BD, Perfect, JR, Gao, J-L, Kullberg, B-J, Netea, MG, and Murphy, PM. "CX3CR1-dependent renal macrophage survival promotes Candida control and host survival." J Clin Invest 123, no. 12 (December 2013): 5035-5051.

PMID
24177428
Full Text

Frazzitta, AE, Vora, H, Price, MS, Tenor, JL, Betancourt-Quiroz, M, Toffaletti, DL, Cheng, N, and Perfect, JR. "Nitrogen source-dependent capsule induction in human-pathogenic cryptococcus species." Eukaryot Cell 12, no. 11 (November 2013): 1439-1450.

PMID
23975889
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Rubach, MP, Pavlisko, EN, and Perfect, JR. "Pericarditis mediated by respiratory syncytial virus in a hematopoietic stem cell transplant patient." Transplant infectious disease : an official journal of the Transplantation Society 15, no. 4 (August 2013): E152-E156.

PMID
23725410
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O'Connor, L, Livermore, J, Sharp, AD, Goodwin, J, Gregson, L, Howard, SJ, Felton, TW, Schwartz, JA, Neely, MN, Harrison, TS, Perfect, JR, and Hope, WW. "Pharmacodynamics of liposomal amphotericin B and flucytosine for cryptococcal meningoencephalitis: safe and effective regimens for immunocompromised patients." J Infect Dis 208, no. 2 (July 15, 2013): 351-361.

PMID
23599314
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Bratton, EW, El Husseini, N, Chastain, CA, Lee, MS, Poole, C, Stürmer, T, Weber, DJ, Juliano, JJ, and Perfect, JR. "Approaches to antifungal therapies and their effectiveness among patients with cryptococcosis." Antimicrob Agents Chemother 57, no. 6 (June 2013): 2485-2495.

PMID
23478968
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Sudan, A, Livermore, J, Howard, SJ, Al-Nakeeb, Z, Sharp, A, Goodwin, J, Gregson, L, Warn, PA, Felton, TW, Perfect, JR, Harrison, TS, and Hope, WW. "Pharmacokinetics and pharmacodynamics of fluconazole for cryptococcal meningoencephalitis: implications for antifungal therapy and in vitro susceptibility breakpoints." Antimicrob Agents Chemother 57, no. 6 (June 2013): 2793-2800.

PMID
23571544
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Perfect, JR. "Efficiently killing a sugar-coated yeast." N Engl J Med 368, no. 14 (April 4, 2013): 1354-1356.

PMID
23550675
Full Text

Pappas, PG, Kontoyiannis, DP, Perfect, JR, and Chiller, TM. "Real-time treatment guidelines: considerations during the Exserohilum rostratum outbreak in the United States." Antimicrob Agents Chemother 57, no. 4 (April 2013): 1573-1576.

PMID
23384532
Full Text

Boehm, RD, Miller, PR, Schell, WA, Perfect, JR, and Narayan, RJ. "Inkjet Printing of Amphotericin B onto Biodegradable Microneedles Using Piezoelectric Inkjet Printing." JOM 65, no. 4 (April 2013): 525-533.

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