John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan at Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., University of Toledo, 1974

Publications

Cronan, MR, Rosenberg, AF, Oehlers, SH, Saelens, JW, Sisk, DM, Jurcic Smith, KL, Lee, S, and Tobin, DM. "CLARITY and PACT-based imaging of adult zebrafish and mouse for whole-animal analysis of infections." Disease models & mechanisms 8, no. 12 (December 2015): 1643-1650.

PMID
26449262
Full Text

Janout, V, Bienvenu, C, Schell, W, Perfect, JR, and Regen, SL. "Correction to Molecular Umbrella-Amphotericin B Conjugates." Bioconjugate Chemistry 26, no. 10 (October 2015): 2168-null.

PMID
26421479
Full Text

Janout, V, Schell, WA, Thévenin, D, Yu, Y, Perfect, JR, and Regen, SL. "Taming Amphotericin B." Bioconjugate Chemistry 26, no. 10 (October 2015): 2021-2024.

PMID
26340430
Full Text

Janout, V, Schell, WA, Thevenin, D, Yu, Y, Perfect, JR, and Regen, SL. "Taming Amphotericin B." BIOCONJUGATE CHEMISTRY 26, no. 10 (October 2015): 2021-2024.

Full Text

Tenor, JL, Oehlers, SH, Yang, JL, Tobin, DM, and Perfect, JR. "Live Imaging of Host-Parasite Interactions in a Zebrafish Infection Model Reveals Cryptococcal Determinants of Virulence and Central Nervous System Invasion." Mbio 6, no. 5 (September 29, 2015): e01425-e01415.

PMID
26419880
Full Text

Wang, L, Oehlers, SH, Espenschied, ST, Rawls, JF, Tobin, DM, and Ko, DC. "CPAG: software for leveraging pleiotropy in GWAS to reveal similarity between human traits links plasma fatty acids and intestinal inflammation." Genome Biology 16 (September 15, 2015): 190-null.

PMID
26374098
Full Text

Clement, ME, Maziarz, EK, Schroder, JN, Patel, CB, and Perfect, JR. "Scedosporium apiosermum infection of the "Native" valve: Fungal endocarditis in an orthotopic heart transplant recipient." Medical mycology case reports 9 (September 2015): 34-36.

PMID
26288748
Full Text

Chen, Y, Litvintseva, AP, Frazzitta, AE, Haverkamp, MR, Wang, L, Fang, C, Muthoga, C, Mitchell, TG, and Perfect, JR. "Comparative analyses of clinical and environmental populations of Cryptococcus neoformans in Botswana." Molecular ecology 24, no. 14 (July 7, 2015): 3559-3571.

PMID
26053414
Full Text

Hart, BE, Asrican, R, Lim, S-Y, Sixsmith, JD, Lukose, R, Souther, SJR, Rayasam, SDG, Saelens, JW, Chen, C-J, Seay, SA, Berney-Meyer, L, Magtanong, L, Vermeul, K, Pajanirassa, P, Jimenez, AE, Ng, TW, Tobin, DM, Porcelli, SA, Larsen, MH, Schmitz, JE, Haynes, BF, Jacobs, WR, Lee, S, and Frothingham, R. "Stable Expression of Lentiviral Antigens by Quality-Controlled Recombinant Mycobacterium bovis BCG Vectors." Clinical and vaccine immunology : CVI 22, no. 7 (July 2015): 726-741.

PMID
25924766
Full Text

Molefi, M, Chofle, AA, Molloy, SF, Kalluvya, S, Changalucha, JM, Cainelli, F, Leeme, T, Lekwape, N, Goldberg, DW, Haverkamp, M, Bisson, GP, Perfect, JR, Letang, E, Fenner, L, Meintjes, G, Burton, R, Makadzange, T, Ndhlovu, CE, Hope, W, Harrison, TS, and Jarvis, JN. "AMBITION-cm: intermittent high dose AmBisome on a high dose fluconazole backbone for cryptococcal meningitis induction therapy in sub-Saharan Africa: study protocol for a randomized controlled trial." Trials 16 (June 17, 2015): 276-.

PMID
26081985
Full Text

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