John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan at Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., University of Toledo, 1974

Publications

Caza, Mélissa, Guanggan Hu, Michael Price, John R. Perfect, and James W. Kronstad. “The Zinc Finger Protein Mig1 Regulates Mitochondrial Function and Azole Drug Susceptibility in the Pathogenic Fungus Cryptococcus neoformans..” Msphere 1, no. 1 (January 2016). https://doi.org/10.1128/mSphere.00080-15.

PMID
27303693
Full Text

Bazemore, Taylor C., Stacey A. Maskarinec, Kahli Zietlow, Edward F. Hendershot, and John R. Perfect. “Familial Adenomatous Polyposis Manifesting as Lactococcus Endocarditis: A Case Report and Review of the Association of Lactococcus with Underlying Gastrointestinal Disease..” Case Rep Infect Dis 2016 (2016). https://doi.org/10.1155/2016/5805326.

PMID
27818810
Full Text

Tenor, Jennifer L., Stefan H. Oehlers, Jialu L. Yang, David M. Tobin, and John R. Perfect. “Live Imaging of Host-Parasite Interactions in a Zebrafish Infection Model Reveals Cryptococcal Determinants of Virulence and Central Nervous System Invasion..” Mbio 6, no. 5 (September 29, 2015): e01425–e01415. https://doi.org/10.1128/mBio.01425-15.

PMID
26419880
Full Text

Clement, Meredith E., Eileen K. Maziarz, Jacob N. Schroder, Chetan B. Patel, and John R. Perfect. “Scedosporium apiosermum infection of the "Native" valve: Fungal endocarditis in an orthotopic heart transplant recipient..” Med Mycol Case Rep 9 (September 2015): 34–36. https://doi.org/10.1016/j.mmcr.2015.07.005.

PMID
26288748
Full Text

Chen, Yuan, Anastasia P. Litvintseva, Aubrey E. Frazzitta, Miriam R. Haverkamp, Liuyang Wang, Charles Fang, Charles Muthoga, Thomas G. Mitchell, and John R. Perfect. “Comparative analyses of clinical and environmental populations of Cryptococcus neoformans in Botswana..” Mol Ecol 24, no. 14 (July 2015): 3559–71. https://doi.org/10.1111/mec.13260.

PMID
26053414
Full Text

Molefi, Mooketsi, Awilly A. Chofle, Síle F. Molloy, Samuel Kalluvya, John M. Changalucha, Francesca Cainelli, Tshepo Leeme, et al. “AMBITION-cm: intermittent high dose AmBisome on a high dose fluconazole backbone for cryptococcal meningitis induction therapy in sub-Saharan Africa: study protocol for a randomized controlled trial..” Trials 16 (June 17, 2015). https://doi.org/10.1186/s13063-015-0799-6.

PMID
26081985
Full Text

Allen, David, Dustin Wilson, Richard Drew, and John Perfect. “Azole antifungals: 35 years of invasive fungal infection management..” Expert Rev Anti Infect Ther 13, no. 6 (June 2015): 787–98. https://doi.org/10.1586/14787210.2015.1032939.

PMID
25843556
Full Text

Pages