S. Munir Alam, PhD

Professor in Medicine
Professor of Pathology
Member of the Duke Human Vaccine Institute
Campus mail 2 Genome Ct, MSRB II - Room 4004, Durham, NC 27710
Phone (919) 668-6372
Email address alam0004@mc.duke.edu

Research Interests.
Biophysical analysis of coreceptor modulation of TCR-MHC interactions. One of our research interests is to study the molecular mechanisms of T cell recognition. We have particular interest in understanding the trimolecular interactions between membrane bound T cell receptor (TCR-CD3 complex) and its ligand, the peptide-MHC complex (pMHC), and co-receptor molecules. We are using different biophysical approaches which include surface plasmon resonance, isothermal titration calorimetry and atomic force spectroscopy to study the influence of co-stimulatory molecules on the kinetics and thermodynamics of TCR-pMHC binding. The goal is to use integrated molecular and biophysical approaches to study membrane TCR-MHC interactions and to understand how these interactions are modulated by co-receptors during T cell development and activation.
Energetics and kinetics of monoclonal antibody binding to soluble HIV Envelope proteins. An additional interest of our lab involves biophysical characterization of soluble, recombinant human HIV-1 Envelope proteins (gp120 and gp140). We have employed surface plasmon resonance, analytical sedimentation equilibrium analysis, isothermal titration calorimetry and atomic force spectroscopy to characterize the antigenicity and study the energetics, kinetics and single molecule force measurements of the binding of anti-gp120 and anti-gp41 antibodies to several recombinant HIV-1 Envelope proteins. These studies have relevance in immunogen design for generating anti-HIV vaccines.

Education and Training

  • Ph.D., University of Glasgow (Scotland), 1992

Publications

Krebs, SJ, Kwon, YD, Schramm, CA, Law, WH, Donofrio, G, Zhou, KH, Gift, S, Dussupt, V, Georgiev, IS, Schätzle, S, McDaniel, JR, Lai, Y-T, Sastry, M, Zhang, B, Jarosinski, MC, Ransier, A, Chenine, AL, Asokan, M, Bailer, RT, Bose, M, Cagigi, A, Cale, EM, Chuang, G-Y, Darko, S, Driscoll, JI, Druz, A, Gorman, J, Laboune, F, Louder, MK, McKee, K, Mendez, L, Moody, MA, O'Sullivan, AM, Owen, C, Peng, D, Rawi, R, Sanders-Buell, E, Shen, C-H, Shiakolas, AR, Stephens, T, Tsybovsky, Y, Tucker, C, Verardi, R, Wang, K, Zhou, J, Zhou, T, Georgiou, G, Alam, SM, Haynes, BF, Rolland, M, Matyas, GR, Polonis, VR, McDermott, AB, Douek, DC, Shapiro, L, Tovanabutra, S, Michael, NL, Mascola, JR, Robb, ML, Kwong, PD, and Doria-Rose, NA. "Longitudinal Analysis Reveals Early Development of Three MPER-Directed Neutralizing Antibody Lineages from an HIV-1-Infected Individual." Immunity 50, no. 3 (March 12, 2019): 677-691.e13.

PMID
30876875
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Dennis, M, Eudailey, J, Pollara, J, McMillan, AS, Cronin, KD, Saha, PT, Curtis, AD, Hudgens, MG, Fouda, GG, Ferrari, G, Alam, M, Van Rompay, KKA, De Paris, K, Permar, S, and Shen, X. "Coadministration of CH31 Broadly Neutralizing Antibody Does Not Affect Development of Vaccine-Induced Anti-HIV-1 Envelope Antibody Responses in Infant Rhesus Macaques." Journal of Virology 93, no. 5 (March 2019).

PMID
30541851
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Bonsignori, M, Scott, E, Wiehe, K, Easterhoff, D, Alam, SM, Hwang, K-K, Cooper, M, Xia, S-M, Zhang, R, Montefiori, DC, Henderson, R, Nie, X, Kelsoe, G, Moody, MA, Chen, X, Joyce, MG, Kwong, PD, Connors, M, Mascola, JR, McGuire, AT, Stamatatos, L, Medina-Ramírez, M, Sanders, RW, Saunders, KO, Kepler, TB, and Haynes, BF. "Inference of the HIV-1 VRC01 Antibody Lineage Unmutated Common Ancestor Reveals Alternative Pathways to Overcome a Key Glycan Barrier." Immunity 49, no. 6 (December 11, 2018): 1162-1174.e8.

PMID
30552024
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Phillips, B, Van Rompay, KKA, Rodriguez-Nieves, J, Lorin, C, Koutsoukos, M, Tomai, M, Fox, CB, Eudailey, J, Dennis, M, Alam, SM, Hudgens, M, Fouda, G, Pollara, J, Moody, A, Shen, X, Ferrari, G, Permar, S, and De Paris, K. "Adjuvant-Dependent Enhancement of HIV Env-Specific Antibody Responses in Infant Rhesus Macaques." Journal of Virology 92, no. 20 (October 2018).

PMID
30089691
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Dennison, SM, Reichartz, M, Seaton, KE, Dutta, S, Wille-Reece, U, Hill, AVS, Ewer, KJ, Rountree, W, Sarzotti-Kelsoe, M, Ozaki, DA, Alam, SM, and Tomaras, GD. "Qualified Biolayer Interferometry Avidity Measurements Distinguish the Heterogeneity of Antibody Interactions with Plasmodium falciparum Circumsporozoite Protein Antigens." Journal of Immunology (Baltimore, Md. : 1950) 201, no. 4 (August 2018): 1315-1326.

PMID
30006374
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Wiehe, K, Bradley, T, Meyerhoff, RR, Hart, C, Williams, WB, Easterhoff, D, Faison, WJ, Kepler, TB, Saunders, KO, Alam, SM, Bonsignori, M, and Haynes, BF. "Functional Relevance of Improbable Antibody Mutations for HIV Broadly Neutralizing Antibody Development." Cell Host & Microbe 23, no. 6 (June 2018): 759-765.e6.

PMID
29861171
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Wills, S, Hwang, K-K, Liu, P, Dennison, SM, Tay, MZ, Shen, X, Pollara, J, Lucas, JT, Parks, R, Rerks-Ngarm, S, Pitisuttithum, P, Nitayapan, S, Kaewkungwal, J, Thomas, R, Kim, JH, Michael, NL, Robb, ML, McRaven, M, Montefiori, DC, Hope, TJ, Liao, H-X, Moody, MA, Ferrari, G, Haynes, BF, Alam, SM, Bonsignori, M, and Tomaras, GD. "HIV-1-Specific IgA Monoclonal Antibodies from an HIV-1 Vaccinee Mediate Galactosylceramide Blocking and Phagocytosis." Journal of Virology 92, no. 7 (April 2018).

PMID
29321320
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Yates, NL, deCamp, AC, Korber, BT, Liao, H-X, Irene, C, Pinter, A, Peacock, J, Harris, LJ, Sawant, S, Hraber, P, Shen, X, Rerks-Ngarm, S, Pitisuttithum, P, Nitayapan, S, Berman, PW, Robb, ML, Pantaleo, G, Zolla-Pazner, S, Haynes, BF, Alam, SM, Montefiori, DC, and Tomaras, GD. "HIV-1 Envelope Glycoproteins from Diverse Clades Differentiate Antibody Responses and Durability among Vaccinees." Journal of Virology 92, no. 8 (April 2018).

PMID
29386288
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Shen, X, Basu, R, Sawant, S, Beaumont, D, Kwa, SF, LaBranche, C, Seaton, KE, Yates, NL, Montefiori, DC, Ferrari, G, Wyatt, LS, Moss, B, Alam, SM, Haynes, BF, Tomaras, GD, and Robinson, HL. "HIV-1 gp120 and Modified Vaccinia Virus Ankara (MVA) gp140 Boost Immunogens Increase Immunogenicity of a DNA/MVA HIV-1 Vaccine." Journal of Virology 91, no. 24 (December 2017).

PMID
29021394
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Saunders, KO, Verkoczy, LK, Jiang, C, Zhang, J, Parks, R, Chen, H, Housman, M, Bouton-Verville, H, Shen, X, Trama, AM, Scearce, R, Sutherland, L, Santra, S, Newman, A, Eaton, A, Xu, K, Georgiev, IS, Joyce, MG, Tomaras, GD, Bonsignori, M, Reed, SG, Salazar, A, Mascola, JR, Moody, MA, Cain, DW, Centlivre, M, Zurawski, S, Zurawski, G, Erickson, HP, Kwong, PD, Alam, SM, Levy, Y, Montefiori, DC, and Haynes, BF. "Vaccine Induction of Heterologous Tier 2 HIV-1 Neutralizing Antibodies in Animal Models." Cell Reports 21, no. 13 (December 2017): 3681-3690.

PMID
29281818
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