S. Munir Alam, PhD

Professor in Medicine
Professor of Pathology
Member of the Duke Human Vaccine Institute
Campus mail 2 Genome Ct, MSRB II - Room 4004, Durham, NC 27710
Phone (919) 668-6372
Email address alam0004@mc.duke.edu

Research Interests.
Biophysical analysis of coreceptor modulation of TCR-MHC interactions. One of our research interests is to study the molecular mechanisms of T cell recognition. We have particular interest in understanding the trimolecular interactions between membrane bound T cell receptor (TCR-CD3 complex) and its ligand, the peptide-MHC complex (pMHC), and co-receptor molecules. We are using different biophysical approaches which include surface plasmon resonance, isothermal titration calorimetry and atomic force spectroscopy to study the influence of co-stimulatory molecules on the kinetics and thermodynamics of TCR-pMHC binding. The goal is to use integrated molecular and biophysical approaches to study membrane TCR-MHC interactions and to understand how these interactions are modulated by co-receptors during T cell development and activation.
Energetics and kinetics of monoclonal antibody binding to soluble HIV Envelope proteins. An additional interest of our lab involves biophysical characterization of soluble, recombinant human HIV-1 Envelope proteins (gp120 and gp140). We have employed surface plasmon resonance, analytical sedimentation equilibrium analysis, isothermal titration calorimetry and atomic force spectroscopy to characterize the antigenicity and study the energetics, kinetics and single molecule force measurements of the binding of anti-gp120 and anti-gp41 antibodies to several recombinant HIV-1 Envelope proteins. These studies have relevance in immunogen design for generating anti-HIV vaccines.

Education and Training

  • Ph.D., University of Glasgow (Scotland), 1992

Publications

Williams, WB, Liao, H-X, Moody, MA, Kepler, TB, Alam, SM, Gao, F, Wiehe, K, Trama, AM, Jones, K, Zhang, R, Song, H, Marshall, DJ, Whitesides, JF, Sawatzki, K, Hua, A, Liu, P, Tay, MZ, Seaton, KE, Shen, X, Foulger, A, Lloyd, KE, Parks, R, Pollara, J, Ferrari, G, Yu, J-S, Vandergrift, N, Montefiori, DC, Sobieszczyk, ME, Hammer, S, Karuna, S, Gilbert, P, Grove, D, Grunenberg, N, McElrath, MJ, Mascola, JR, Koup, RA, Corey, L, Nabel, GJ, Morgan, C, Churchyard, G, Maenza, J, Keefer, M, and Graham, BS et al. "HIV-1 VACCINES. Diversion of HIV-1 vaccine-induced immunity by gp41-microbiota cross-reactive antibodies." Science (New York, N.Y.) 349, no. 6249 (August 2015): aab1253-.

PMID
26229114
Full Text

Permar, SR, Fong, Y, Vandergrift, N, Fouda, GG, Gilbert, P, Parks, R, Jaeger, FH, Pollara, J, Martelli, A, Liebl, BE, Lloyd, K, Yates, NL, Overman, RG, Shen, X, Whitaker, K, Chen, H, Pritchett, J, Solomon, E, Friberg, E, Marshall, DJ, Whitesides, JF, Gurley, TC, Von Holle, T, Martinez, DR, Cai, F, Kumar, A, Xia, S-M, Lu, X, Louzao, R, Wilkes, S, Datta, S, Sarzotti-Kelsoe, M, Liao, H-X, Ferrari, G, Alam, SM, Montefiori, DC, Denny, TN, Moody, MA, Tomaras, GD, Gao, F, and Haynes, BF. "Maternal HIV-1 envelope-specific antibody responses and reduced risk of perinatal transmission." The Journal of clinical investigation 125, no. 7 (July 2015): 2702-2706.

PMID
26053661
Full Text

Nicely, NI, Wiehe, K, Kepler, TB, Jaeger, FH, Dennison, SM, Rerks-Ngarm, S, Nitayaphan, S, Pitisuttithum, P, Kaewkungwal, J, Robb, ML, O'Connell, RJ, Michael, NL, Kim, JH, Liao, H-X, Munir Alam, S, Hwang, K-K, Bonsignori, M, and Haynes, BF. "Structural analysis of the unmutated ancestor of the HIV-1 envelope V2 region antibody CH58 isolated from an RV144 vaccine efficacy trial vaccinee." EBioMedicine 2, no. 7 (July 2015): 713-722.

PMID
26288844
Full Text

Hulot, SL, Korber, B, Giorgi, EE, Vandergrift, N, Saunders, KO, Balachandran, H, Mach, LV, Lifton, MA, Pantaleo, G, Tartaglia, J, Phogat, S, Jacobs, B, Kibler, K, Perdiguero, B, Gomez, CE, Esteban, M, Rosati, M, Felber, BK, Pavlakis, GN, Parks, R, Lloyd, K, Sutherland, L, Scearce, R, Letvin, NL, Seaman, MS, Alam, SM, Montefiori, D, Liao, H-X, Haynes, BF, and Santra, S. "Comparison of Immunogenicity in Rhesus Macaques of Transmitted-Founder, HIV-1 Group M Consensus, and Trivalent Mosaic Envelope Vaccines Formulated as a DNA Prime, NYVAC, and Envelope Protein Boost." Journal of virology 89, no. 12 (June 2015): 6462-6480.

PMID
25855741
Full Text

Fusco, WG, Choudhary, NR, Stewart, SM, Alam, SM, Sempowski, GD, Elkins, C, and Leduc, I. "Defining Potential Vaccine Targets of Haemophilus ducreyi Trimeric Autotransporter Adhesin DsrA." Monoclonal antibodies in immunodiagnosis and immunotherapy 34, no. 2 (April 2015): 73-82.

PMID
25897604
Full Text

Liu, M, Yang, G, Wiehe, K, Nicely, NI, Vandergrift, NA, Rountree, W, Bonsignori, M, Alam, SM, Gao, J, Haynes, BF, and Kelsoe, G. "Polyreactivity and autoreactivity among HIV-1 antibodies." Journal of virology 89, no. 1 (January 2015): 784-798.

PMID
25355869
Full Text

Wiehe, K, Easterhoff, D, Luo, K, Nicely, NI, Bradley, T, Jaeger, FH, Dennison, SM, Zhang, R, Lloyd, KE, Stolarchuk, C, Parks, R, Sutherland, LL, Scearce, RM, Morris, L, Kaewkungwal, J, Nitayaphan, S, Pitisuttithum, P, Rerks-Ngarm, S, Sinangil, F, Phogat, S, Michael, NL, Kim, JH, Kelsoe, G, Montefiori, DC, Tomaras, GD, Bonsignori, M, Santra, S, Kepler, TB, Alam, SM, Moody, MA, Liao, H-X, and Haynes, BF. "Antibody light-chain-restricted recognition of the site of immune pressure in the RV144 HIV-1 vaccine trial is phylogenetically conserved." Immunity 41, no. 6 (December 2014): 909-918.

PMID
25526306
Full Text

Huang, J, Kang, BH, Pancera, M, Lee, JH, Tong, T, Feng, Y, Imamichi, H, Georgiev, IS, Chuang, G-Y, Druz, A, Doria-Rose, NA, Laub, L, Sliepen, K, van Gils, MJ, de la Peña, AT, Derking, R, Klasse, P-J, Migueles, SA, Bailer, RT, Alam, M, Pugach, P, Haynes, BF, Wyatt, RT, Sanders, RW, Binley, JM, Ward, AB, Mascola, JR, Kwong, PD, and Connors, M. "Broad and potent HIV-1 neutralization by a human antibody that binds the gp41-gp120 interface." Nature 515, no. 7525 (November 2014): 138-142.

PMID
25186731
Full Text

Hardy, GJ, Wong, GC, Nayak, R, Anasti, K, Hirtz, M, Shapter, JG, Alam, SM, and Zauscher, S. "HIV-1 antibodies and vaccine antigen selectively interact with lipid domains." Biochimica et biophysica acta 1838, no. 10 (October 2014): 2662-2669.

PMID
25019685
Full Text

Kepler, TB, Liao, H-X, Alam, SM, Bhaskarabhatla, R, Zhang, R, Yandava, C, Stewart, S, Anasti, K, Kelsoe, G, Parks, R, Lloyd, KE, Stolarchuk, C, Pritchett, J, Solomon, E, Friberg, E, Morris, L, Karim, SSA, Cohen, MS, Walter, E, Moody, MA, Wu, X, Altae-Tran, HR, Georgiev, IS, Kwong, PD, Boyd, SD, Fire, AZ, Mascola, JR, and Haynes, BF. "Immunoglobulin gene insertions and deletions in the affinity maturation of HIV-1 broadly reactive neutralizing antibodies." Cell host & microbe 16, no. 3 (September 2014): 304-313.

PMID
25211073
Full Text

Pages