S. Munir Alam, PhD

Professor in Medicine
Professor of Pathology
Member of the Duke Human Vaccine Institute
Campus mail 2 Genome Ct, MSRB II - Room 4004, Durham, NC 27710
Phone (919) 668-6372
Email address alam0004@mc.duke.edu

Research Interests.
Biophysical analysis of coreceptor modulation of TCR-MHC interactions. One of our research interests is to study the molecular mechanisms of T cell recognition. We have particular interest in understanding the trimolecular interactions between membrane bound T cell receptor (TCR-CD3 complex) and its ligand, the peptide-MHC complex (pMHC), and co-receptor molecules. We are using different biophysical approaches which include surface plasmon resonance, isothermal titration calorimetry and atomic force spectroscopy to study the influence of co-stimulatory molecules on the kinetics and thermodynamics of TCR-pMHC binding. The goal is to use integrated molecular and biophysical approaches to study membrane TCR-MHC interactions and to understand how these interactions are modulated by co-receptors during T cell development and activation.
Energetics and kinetics of monoclonal antibody binding to soluble HIV Envelope proteins. An additional interest of our lab involves biophysical characterization of soluble, recombinant human HIV-1 Envelope proteins (gp120 and gp140). We have employed surface plasmon resonance, analytical sedimentation equilibrium analysis, isothermal titration calorimetry and atomic force spectroscopy to characterize the antigenicity and study the energetics, kinetics and single molecule force measurements of the binding of anti-gp120 and anti-gp41 antibodies to several recombinant HIV-1 Envelope proteins. These studies have relevance in immunogen design for generating anti-HIV vaccines.

Education and Training

  • Ph.D., University of Glasgow (Scotland), 1992

Publications

Bonsignori, Mattia, Tongqing Zhou, Zizhang Sheng, Lei Chen, Feng Gao, M Gordon Joyce, Gabriel Ozorowski, et al. “Maturation Pathway from Germline to Broad HIV-1 Neutralizer of a CD4-Mimic Antibody..” Cell 165, no. 2 (April 7, 2016): 449–63. https://doi.org/10.1016/j.cell.2016.02.022.

PMID
26949186
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Jeffries, T. L., C. R. Sacha, J. Pollara, J. Himes, F. H. Jaeger, S. M. Dennison, E. McGuire, et al. “The function and affinity maturation of HIV-1 gp120-specific monoclonal antibodies derived from colostral B cells..” Mucosal Immunol 9, no. 2 (March 2016): 414–27. https://doi.org/10.1038/mi.2015.70.

PMID
26242599
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Wang, Yuge, Pratibha Kapoor, Robert Parks, Aaron Silva-Sanchez, S Munir Alam, Laurent Verkoczy, Hua-Xin Liao, et al. “HIV-1 gp140 epitope recognition is influenced by immunoglobulin DH gene segment sequence..” Immunogenetics 68, no. 2 (February 2016): 145–55. https://doi.org/10.1007/s00251-015-0890-x.

PMID
26687685
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Bradley, Todd, Daniela Fera, Jinal Bhiman, Leila Eslamizar, Xiaozhi Lu, Kara Anasti, Ruijung Zhang, et al. “Structural Constraints of Vaccine-Induced Tier-2 Autologous HIV Neutralizing Antibodies Targeting the Receptor-Binding Site..” Cell Rep 14, no. 1 (January 5, 2016): 43–54. https://doi.org/10.1016/j.celrep.2015.12.017.

PMID
26725118
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Mansour, Robin G., Lisa Stamper, Frederick Jaeger, Erin McGuire, Genevieve Fouda, Joshua Amos, Kimberly Barbas, et al. “The Presence and Anti-HIV-1 Function of Tenascin C in Breast Milk and Genital Fluids..” Plos One 11, no. 5 (2016). https://doi.org/10.1371/journal.pone.0155261.

PMID
27182834
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Soto, Cinque, Gilad Ofek, M Gordon Joyce, Baoshan Zhang, Krisha McKee, Nancy S. Longo, Yongping Yang, et al. “Developmental Pathway of the MPER-Directed HIV-1-Neutralizing Antibody 10E8..” Plos One 11, no. 6 (2016). https://doi.org/10.1371/journal.pone.0157409.

PMID
27299673
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Hraber, Peter, Bette Korber, Kshitij Wagh, Elena E. Giorgi, Tanmoy Bhattacharya, S. Gnanakaran, Alan S. Lapedes, et al. “Longitudinal Antigenic Sequences and Sites from Intra-Host Evolution (LASSIE) Identifies Immune-Selected HIV Variants..” Viruses 7, no. 10 (October 21, 2015): 5443–75. https://doi.org/10.3390/v7102881.

PMID
26506369
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Moody, M Anthony, Feng Gao, Thaddeus C. Gurley, Joshua D. Amos, Amit Kumar, Bhavna Hora, Dawn J. Marshall, et al. “Strain-Specific V3 and CD4 Binding Site Autologous HIV-1 Neutralizing Antibodies Select Neutralization-Resistant Viruses..” Cell Host Microbe 18, no. 3 (September 9, 2015): 354–62. https://doi.org/10.1016/j.chom.2015.08.006.

PMID
26355218
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Williams, Wilton B., Hua-Xin Liao, M Anthony Moody, Thomas B. Kepler, S Munir Alam, Feng Gao, Kevin Wiehe, et al. “HIV-1 VACCINES. Diversion of HIV-1 vaccine-induced immunity by gp41-microbiota cross-reactive antibodies..” Science 349, no. 6249 (August 14, 2015). https://doi.org/10.1126/science.aab1253.

PMID
26229114
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Wieczorek, Lindsay, Shelly J. Krebs, Vaniambadi Kalyanaraman, Stephen Whitney, Sodsai Tovanabutra, Carlos G. Moscoso, Eric Sanders-Buell, et al. “Comparable Antigenicity and Immunogenicity of Oligomeric Forms of a Novel, Acute HIV-1 Subtype C gp145 Envelope for Use in Preclinical and Clinical Vaccine Research..” J Virol 89, no. 15 (August 2015): 7478–93. https://doi.org/10.1128/JVI.00412-15.

PMID
25972551
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