S. Munir Alam, PhD

Professor in Medicine
Professor of Pathology
Member of the Duke Human Vaccine Institute
Campus mail 2 Genome Ct, MSRB II - Room 4004, Durham, NC 27710
Phone (919) 668-6372
Email address alam0004@mc.duke.edu

Research Interests.
Biophysical analysis of coreceptor modulation of TCR-MHC interactions. One of our research interests is to study the molecular mechanisms of T cell recognition. We have particular interest in understanding the trimolecular interactions between membrane bound T cell receptor (TCR-CD3 complex) and its ligand, the peptide-MHC complex (pMHC), and co-receptor molecules. We are using different biophysical approaches which include surface plasmon resonance, isothermal titration calorimetry and atomic force spectroscopy to study the influence of co-stimulatory molecules on the kinetics and thermodynamics of TCR-pMHC binding. The goal is to use integrated molecular and biophysical approaches to study membrane TCR-MHC interactions and to understand how these interactions are modulated by co-receptors during T cell development and activation.
Energetics and kinetics of monoclonal antibody binding to soluble HIV Envelope proteins. An additional interest of our lab involves biophysical characterization of soluble, recombinant human HIV-1 Envelope proteins (gp120 and gp140). We have employed surface plasmon resonance, analytical sedimentation equilibrium analysis, isothermal titration calorimetry and atomic force spectroscopy to characterize the antigenicity and study the energetics, kinetics and single molecule force measurements of the binding of anti-gp120 and anti-gp41 antibodies to several recombinant HIV-1 Envelope proteins. These studies have relevance in immunogen design for generating anti-HIV vaccines.

Education and Training

  • Ph.D., University of Glasgow (Scotland), 1992

Publications

Nicely, NI, Dennison, SM, Spicer, L, Scearce, RM, Kelsoe, G, Ueda, Y, Chen, H, Liao, H-X, Alam, SM, and Haynes, BF. "Crystal structure of a non-neutralizing antibody to the HIV-1 gp41 membrane-proximal external region." Nature structural & molecular biology 17, no. 12 (December 2010): 1492-1494.

PMID
21076400
Full Text

Haynes, BF, Nicely, NI, and Alam, SM. "HIV-1 autoreactive antibodies: are they good or bad for HIV-1 prevention?." Nature structural & molecular biology 17, no. 5 (May 2010): 543-545.

PMID
20442740
Full Text

Zhang, M-Y, Borges, AR, Ptak, RG, Wang, Y, Dimitrov, AS, Alam, SM, Wieczorek, L, Bouma, P, Fouts, T, Jiang, S, Polonis, VR, Haynes, BF, Quinnan, GV, Montefiori, DC, and Dimitrov, DS. "Potent and broad neutralizing activity of a single chain antibody fragment against cell-free and cell-associated HIV-1." MAbs 2, no. 3 (May 2010): 266-274.

PMID
20305395
Scholars@Duke

Moody, MA, Liao, H-X, Alam, SM, Scearce, RM, Plonk, MK, Kozink, DM, Drinker, MS, Zhang, R, Xia, S-M, Sutherland, LL, Tomaras, GD, Giles, IP, Kappes, JC, Ochsenbauer-Jambor, C, Edmonds, TG, Soares, M, Barbero, G, Forthal, DN, Landucci, G, Chang, C, King, SW, Kavlie, A, Denny, TN, Hwang, K-K, Chen, PP, Thorpe, PE, Montefiori, DC, and Haynes, BF. "Anti-phospholipid human monoclonal antibodies inhibit CCR5-tropic HIV-1 and induce beta-chemokines." The Journal of Experimental Medicine 207, no. 4 (April 5, 2010): 763-776.

PMID
20368576
Full Text

Shen, X, Dennison, SM, Liu, P, Gao, F, Jaeger, F, Montefiori, DC, Verkoczy, L, Haynes, BF, Alam, SM, and Tomaras, GD. "Prolonged exposure of the HIV-1 gp41 membrane proximal region with L669S substitution." Proc Natl Acad Sci U S A 107, no. 13 (March 30, 2010): 5972-5977.

PMID
20231447
Full Text

Verkoczy, L, Diaz, M, Holl, TM, Ouyang, Y-B, Bouton-Verville, H, Alam, SM, Liao, H-X, Kelsoe, G, and Haynes, BF. "Autoreactivity in an HIV-1 broadly reactive neutralizing antibody variable region heavy chain induces immunologic tolerance." Proc Natl Acad Sci U S A 107, no. 1 (January 5, 2010): 181-186.

PMID
20018688
Full Text

Saaem, I, Ma, K-S, Alam, SM, and Tian, J. "Fabrication of plastic biochips." Journal of Vacuum Science and Technology A: Vacuum, Surfaces and Films 28, no. 4 (2010): 963-968.

Full Text

Alam, SM, Morelli, M, Dennison, SM, Liao, H-X, Zhang, R, Xia, S-M, Rits-Volloch, S, Sun, L, Harrison, SC, Haynes, BF, and Chen, B. "Role of HIV membrane in neutralization by two broadly neutralizing antibodies." Proc Natl Acad Sci U S A 106, no. 48 (December 1, 2009): 20234-20239.

PMID
19906992
Full Text

Gao, F, Scearce, RM, Alam, SM, Hora, B, Xia, S, Hohm, JE, Parks, RJ, Ogburn, DF, Tomaras, GD, Park, E, Lomas, WE, Maino, VC, Fiscus, SA, Cohen, MS, Moody, MA, Hahn, BH, Korber, BT, Liao, H-X, and Haynes, BF. "Cross-reactive monoclonal antibodies to multiple HIV-1 subtype and SIVcpz envelope glycoproteins." Virology 394, no. 1 (November 2009): 91-98.

PMID
19744690
Full Text

Verkoczy, L, Moody, MA, Holl, TM, Bouton-Verville, H, Scearce, RM, Hutchinson, J, Alam, SM, Kelsoe, G, and Haynes, BF. "Functional, non-clonal IgMa-restricted B cell receptor interactions with the HIV-1 envelope gp41 membrane proximal external region. (Published online)" PLoS One 4, no. 10 (October 6, 2009): e7215-.

PMID
19806186
Full Text

Pages