Bryan R. Cullen, PhD

Professor of Molecular Genetics and Microbiology
James B. Duke Professor of Molecular Genetics and Microbiology
Director, Center for Virology
Professor in Medicine
Member of the Duke Cancer Institute
Campus mail 0424 Clin & Res Labs, Durham, NC 27710
Phone (919) 684-3369
Email address bryan.cullen@duke.edu

My laboratory has for sometime been interested in understanding the molecular biology of the replication cycle of the pathogenic retrovirus HIV-1. Because HIV-1 gene expression is primarily regulated by specific RNA:protein interactions, my laboratory has also become interested in the more general area of RNA sequence mediated gene regulation, including nuclear mRNA export and the phenomenon of RNA interference.

In the past, my laboratory has worked extensively on Tat, the transcriptional regulator encoded by HIV-1, and on Rev, a virally encoded nuclear mRNA export factor. Our major focus at present is a third HIV-1 regulatory protein termed Vif. In the absence of Vif, HIV-1 virions are produced normally but are largely non-infectious. It has now been demonstrated that Vif functions to block an innate human antiretroviral defense pathway that relies on a factor called APOBEC3G or CEM15. In the absence of Vif, APOBEC3G is packaged into virions and induces degradation of the HIV-1 genome during reverse transcription in target cells. Vif directly interacts with APOBEC3G and thereby allows reverse transcription to proceed unimpeded. Among other issues, we are currently interested in how APOBEC3G is packaged into virions and in how Vif blocks APOBEC3G function. The role of APOBEC3G in cellular defense against other retroviruses and retrotransposons is also an area of interest.

A second major research area in my group relates to how microRNA precursors are processed to yield mature microRNAs and how microRNAs, and the closely related small interfering RNAs, function in human cells. We were the first group to demonstrate overexpression of human microRNAs and therefore have a system in place which should allow us to make rapid progress in this area. We also remain interested in using RNA interference to determine the role of specific cellular factors in different processes, including HIV-1 replication and nuclear mRNA export.

Education and Training

  • Ph.D., University of Medicine and Dentistry of New Jersey, 1984

Publications

Cullen, BR, Kopchick, JJ, and Stacey, DW. "Effect of intron size on splicing efficiency in retroviral transcripts." Nucleic Acids Res 10, no. 19 (October 11, 1982): 6177-6190.

PMID
6292870
Scholars@Duke

Kopchick, JJ, Cullen, BR, and Stacey, DW. "Rapid analysis of small nucleic acid samples by gel electrophoresis." Anal Biochem 115, no. 2 (August 1981): 419-423.

PMID
6272606
Scholars@Duke

Fasy, TM, Cullen, BR, Luk, D, and Bick, MD. "Studies on the enhanced interaction of halodeoxyuridine-substituted DNAs with H1 histones and other polypeptides." J Biol Chem 255, no. 4 (February 25, 1980): 1380-1387.

PMID
7354034
Scholars@Duke

Cullen, BR, Emigholz, K, and Monahan, JJ. "Protein patterns of early mouse embryos during development." Differentiation 17, no. 3 (1980): 151-160.

PMID
7450326
Scholars@Duke

Alexander, A, Cullen, B, Emigholz, K, Norgard, MV, and Monahan, JJ. "A computer program for displaying two-dimensional gel electrophoresis data." Analytical Biochemistry 103, no. 1 (1980): 176-183.

PMID
7377541
Scholars@Duke

Cullen, B, Emigholz, K, and Monahan, J. "The transient appearance of specific proteins in one-cell mouse embryos." Developmental Biology 76, no. 1 (1980): 215-221.

PMID
7380093
Scholars@Duke

Cullen, BR, and Bick, MD. "Bromodeoxyuridine induction of deoxycytidine deaminase activity in a hamster cell line." Biochim Biophys Acta 517, no. 1 (January 26, 1978): 158-168.

PMID
623754
Scholars@Duke

Bick, MD, and Cullen, BR. "Bromodeoxyuridine inhibition of Friend leukemia cell induction by butyric acid: Time course of inhibition, reversal, and effect of other base analogs." Somatic Cell Genetics 2, no. 6 (November 1, 1976): 545-558.

Full Text

Cullen, BR, and Bick, MD. "Thermal denaturation of DNA from bromodeoxyuridine substituted cells." Nucleic Acids Res 3, no. 1 (January 1976): 49-62.

PMID
1250706
Scholars@Duke

CULLEN, BR. "Surgery of the gallbladder; postoperative study of 150 cases." Ann West Med Surg 3, no. 9 (September 1949): 318-321.

PMID
18139427
Scholars@Duke

Pages