John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan at Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., University of Toledo, 1974


Wright, K. A., J. R. Perfect, and W. Ritter. “The pharmacokinetics of BAY R3783 and its efficacy in the treatment of experimental cryptococcal meningitis..” J Antimicrob Chemother 26, no. 3 (September 1990): 387–97.

Full Text

Hobbs, M. M., J. R. Perfect, D. L. Granger, and D. T. Durack. “Opsonic activity of cerebrospinal fluid in experimental cryptococcal meningitis..” Infect Immun 58, no. 7 (July 1990): 2115–19.


Wong, B., J. R. Perfect, S. Beggs, and K. A. Wright. “Production of the hexitol D-mannitol by Cryptococcus neoformans in vitro and in rabbits with experimental meningitis..” Infect Immun 58, no. 6 (June 1990): 1664–70.


GRANGER, D. L., J. B. HIBBS, J. R. PERFECT, and D. T. DURACK. “MACROPHAGE OXIDATION OF L-ARGININE IS LINKED TO FUNGISTATIC CAPABILITY.” In Microbial Determinants of Virulence and Host Response, edited by E. M. AYOUB and G. H. CASSELL, 147–62. AMER SOC MICROBIOLOGY, 1990.


Granger, D. L., J. B. Hibbs, J. R. Perfect, and D. T. Durack. “Metabolic fate of L-arginine in relation to microbiostatic capability of murine macrophages..” J Clin Invest 85, no. 1 (January 1990): 264–73.

Full Text

Perfect, J. R. “Antifungal therapy and its use in surgical treatment..” Surg Gynecol Obstet 171 Suppl (1990): 41–48.