John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan at Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., University of Toledo, 1974

Publications

GRANGER, D. L., J. R. PERFECT, and D. T. DURACK. “SERUM FACTOR(S) WHICH PROMOTES MACROPHAGE FUNGISTATIC ACTION.” In Journal of Leukocyte Biology, 38:133–133. FEDERATION AMER SOC EXP BIOL, 1985.

Scholars@Duke

GRANGER, D. L., J. R. PERFECT, and D. T. DURACK. “MACROPHAGES REQUIRE A SERUM FACTOR(S) FOR EFFICIENT FUNGISTASIS.” In Clinical Research, 33:A565–A565. SLACK INC, 1985.

Scholars@Duke

Smego, R. A., J. R. Perfect, and D. T. Durack. “Combined therapy with amphotericin B and 5-fluorocytosine for Candida meningitis..” Rev Infect Dis 6, no. 6 (November 1984): 791–801.

PMID
6522917
Scholars@Duke

Smego, R. A., P. W. Devoe, H. A. Sampson, J. R. Perfect, C. M. Wilfert, and R. H. Buckley. “Candida meningitis in two children with severe combined immunodeficiency..” The Journal of Pediatrics 104, no. 6 (June 1984): 902–4. https://doi.org/10.1016/s0022-3476(84)80493-x.

PMID
6726524
Full Text

GRANGER, D. L., J. R. PERFECT, and D. T. DURACK. “CARBON-DIOXIDE REGULATES CAPSULE PRODUCTION BY CRYPTOCOCCUS-NEOFORMANS.” In Clinical Research, 32:A369–A369. SLACK INC, 1984.

Scholars@Duke

PERFECT, J. R., D. L. GRANGER, and D. T. DURACK. “CYCLOSPORINE PREDISPOSES TO EXPERIMENTAL CRYPTOCOCCAL MENINGITIS.” In Clinical Research, 32:A379–A379. SLACK INC, 1984.

Scholars@Duke

Kerr, C. M., J. R. Perfect, P. C. Craven, J. H. Jorgensen, D. J. Drutz, J. D. Shelburne, H. A. Gallis, and R. A. Gutman. “Fungal peritonitis in patients on continuous ambulatory peritoneal dialysis..” Ann Intern Med 99, no. 3 (September 1983): 334–36.

PMID
6351688
Scholars@Duke

Washburn, D. E., J. R. Perfect, and D. T. Durack. “Pharmacokinetics of N-formimidoyl thienamycin and influence of a renal dipeptidase inhibitor in experimental meningitis..” J Antimicrob Chemother 12, no. 1 (July 1983): 39–45. https://doi.org/10.1093/jac/12.1.39.

PMID
6578210
Full Text

Seaworth, B. J., K. J. Kwon-Chung, J. D. Hamilton, and J. R. Perfect. “Brain abscess caused by a variety of cladosporium trichoides..” Am J Clin Pathol 79, no. 6 (June 1983): 747–52. https://doi.org/10.1093/ajcp/79.6.747.

PMID
6221655
Full Text

Pages