John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan at Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., University of Toledo, 1974

Publications

Durack, D. T., G. R. Corey, and J. R. Perfect. “Trimethoprim-sulfamethoxazole vs. ampicillin in the treatment of experimental meningitis due to Streptococcus pneumoniae..” Rev Infect Dis 4, no. 2 (March 1982): 311–14.

PMID
6981157
Scholars@Duke

Cohen, J., J. R. Perfect, and D. T. Durack. “Method for the purification of filobasidiella neoformans basidiospores by flow cytometry.” Medical Mycology 20, no. 3 (January 1, 1982): 245–49. https://doi.org/10.1080/00362178285380351.

Full Text

Durack, D., J. Perfect, J. Hess, and J. Dankert. “Tolerance in viridans streptococci exerts a major influence on efficacy of penicillin in vivo.” Clinical Research 30, no. 2 (January 1, 1982).

Scholars@Duke

Perfect, J. R., S. D. Lang, and D. T. Durack. “Influence of agglutinating antibody in experimental cryptococcal meningitis..” Br J Exp Pathol 62, no. 6 (December 1981): 595–99.

PMID
7034765
Scholars@Duke

Perfect, J. R., and D. T. Durack. “Pharmacokinetics of cefoperazone, moxalactam, cefotaxime, trimethoprim and sulphamethoxazole in experimental meningitis..” J Antimicrob Chemother 8, no. 1 (July 1981): 49–58. https://doi.org/10.1093/jac/8.1.49.

PMID
6265429
Full Text

PERFECT, J. R., D. T. DURACK, and H. A. GALLIS. “CRYPTOCOCCEMIA.” In Clinical Research, 29:A393–A393. SLACK INC, 1981.

Scholars@Duke

Perfect, J. R., S. D. Lang, and D. T. Durack. “Chronic cryptococcal meningitis: a new experimental model in rabbits..” Am J Pathol 101, no. 1 (October 1980): 177–94.

PMID
7004196
Scholars@Duke

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