John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan at Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., University of Toledo, 1974

Publications

Kerr, CM, Perfect, JR, Craven, PC, Jorgensen, JH, Drutz, DJ, Shelburne, JD, Gallis, HA, and Gutman, RA. "Fungal peritonitis in patients on continuous ambulatory peritoneal dialysis." Ann Intern Med 99, no. 3 (September 1983): 334-336. (Review)

PMID
6351688
Scholars@Duke

Washburn, DE, Perfect, JR, and Durack, DT. "Pharmacokinetics of N-formimidoyl thienamycin and influence of a renal dipeptidase inhibitor in experimental meningitis." J Antimicrob Chemother 12, no. 1 (July 1983): 39-45.

PMID
6578210
Scholars@Duke

Seaworth, BJ, Kwon-Chung, KJ, Hamilton, JD, and Perfect, JR. "Brain abscess caused by a variety of cladosporium trichoides." Am J Clin Pathol 79, no. 6 (June 1983): 747-752.

PMID
6221655
Scholars@Duke

Perfect, JR, Durack, DT, and Gallis, HA. "Cryptococcemia." Medicine (Baltimore) 62, no. 2 (March 1983): 98-109.

PMID
6338344
Scholars@Duke

Curiel, TJ, Perfect, JR, and Durack, DT. "Leukocyte subpopulations in cerebrospinal fluid of normal rabbits." Lab Anim Sci 32, no. 6 (December 1982): 622-624.

PMID
6984476
Scholars@Duke

Cohen, J, Perfect, JR, and Durack, DT. "Method for the purification of Filobasidiella neoformans basidiospores by flow cytometry." Sabouraudia 20, no. 3 (September 1982): 245-249.

PMID
6753191
Scholars@Duke

Perfect, JR, and Durack, DT. "Treatment of experimental cryptococcal meningitis with amphotericin B, 5-fluorocytosine, and ketoconazole." J Infect Dis 146, no. 3 (September 1982): 429-435.

PMID
6286799
Scholars@Duke

Perfect, JR, Durack, DT, Hamilton, JD, and Gallis, HA. "Failure of ketoconazole in cryptococcal meningitis." JAMA 247, no. 24 (June 25, 1982): 3349-3351.

PMID
6283185
Scholars@Duke

Cohen, J, Perfect, JR, and Durack, DT. "Cryptococcosis and the basidiospore." Lancet 1, no. 8284 (June 5, 1982): 1301-. (Letter)

PMID
6123035
Scholars@Duke

Durack, DT, Corey, GR, and Perfect, JR. "Trimethoprim-sulfamethoxazole vs. ampicillin in the treatment of experimental meningitis due to Streptococcus pneumoniae." Rev Infect Dis 4, no. 2 (March 1982): 311-314.

PMID
6981157
Scholars@Duke

Pages