Michael Dee Gunn, MD

Professor of Medicine
Professor in Immunology
Associate Professor in Pathology
Campus mail 346 Sands Bldg, Durham, NC 27710
Phone (919) 681-0840
Email address michael.gunn@duke.edu

The focus of my work is on understanding how dendritic cells, monocytes, and macrophages regulate immune responses, contribute to specific disease pathologies, and can be manipulated to stimulate or inhibit specific immune responses. We are also using our knowledge of immunology to develop diagnostics and therapeutics for a variety of human diseases. 

Lab History 

The lab started with our discovery of the lymphoid chemokines, which regulate the migration of lymphocytes and dendritic cells to and within secondary lymphoid organs.  We identified the chemokine (CCL21) that mediates the entry of naïve T cells and activated dendritic cells into lymph nodes and the chemokine (CXCL13) that mediates the entry of B cells into lymphoid follicles.  Our focus then shifted to understanding how specific cell types, primarily dendritic cells, and cell migration events regulate immune responses.  We identified murine plasmacytoid dendritic cells, the cell type that causes pulmonary immune pathology during influenza infection, the dendritic cell type that stimulates Th1 immune responses, and the cell type that induces neuronal injury in Alzheimer's disease.  Our current work continues these basic studies while applying our findings to models of human disease. 

Current Research 

Identification and characterization of inflammatory cell populations in models of human disease – We have developed advanced methods of flow cytometric analysis that allows us to quantify and fully characterize all inflammatory cell types in murine and human tissues.  Using these methods, we are working to identify the cells that mediate a variety of immune pathologies.  Examples include the identification of immune-stimulatory and immune-suppressive cell types in brain tumors, identification of the cells that induce vascular changes pulmonary hypertension, and characterization of the inflammatory response to a variety of infectious pathogens.

Tumor immune therapeutics – We have developed a novel cellular vaccine strategy for the treatment of cancer.  This strategy is much simpler, more cost effective, more clinically feasible, and much more efficacious than classic dendritic cell vaccines.  We are now testing this vaccine in various preclinical tumor models including melanoma and glioblastoma and will soon be advancing it to initial human clinical trials.

Treatment of Acute Lung Injury – We have identified the first small molecule pharmacologic agent that is effective in reducing respiratory dysfunction, vascular leak, tissue injury, and mortality during Acute Lung Injury.  We are currently validating this agent in animal models of chemical-induced ALI and testing its efficacy in reducing ALI caused by other agents such as influenza and smoke inhalation.  We hope to develop this agent as the first effective pharmacologic treatment for ALI in humans.

Development of recombinant antibodies as diagnostic reagents – Our lab has developed novel methods to generate recombinant single chain antibodies using phage display technology.  We are currently using these methods to generate pathogen-specific antibodies for use in diagnostic tests for a variety of human bacterial, viral, and fungal infections.  In collaboration with Duke Biomedical Engineering, we are testing the use of our antibodies in a novel diagnostic assay platform to develop point-of-care assays for the diagnosis of infections by agents such as Zika virus, Dengue virus, Salmonella typhi, and Aspergillus fumigatus.

Education and Training

  • Fellowship in Cardiology, Cardiology, University of California - San Francisco, 1197
  • Internship and Residency, Internal Medicine, Parkland Health & Hospital System, 1197
  • M.D., UT Southwestern Medical School, 1983

Publications

Tangemann, K, Gunn, MD, Giblin, P, and Rosen, SD. "A high endothelial cell-derived chemokine induces rapid, efficient, and subset-selective arrest of rolling T lymphocytes on a reconstituted endothelial substrate." Journal of Immunology (Baltimore, Md. : 1950) 161, no. 11 (December 1998): 6330-6337.

PMID
9834123
Scholars@Duke

Pachynski, RK, Wu, SW, Gunn, MD, and Erle, DJ. "Secondary lymphoid-tissue chemokine (SLC) stimulates integrin alpha 4 beta 7-mediated adhesion of lymphocytes to mucosal addressin cell adhesion molecule-1 (MAdCAM-1) under flow." Journal of Immunology (Baltimore, Md. : 1950) 161, no. 2 (July 1998): 952-956.

PMID
9670974
Scholars@Duke

Nielsen, LB, Véniant, M, Borén, J, Raabe, M, Wong, JS, Tam, C, Flynn, L, Vanni-Reyes, T, Gunn, MD, Goldberg, IJ, Hamilton, RL, and Young, SG. "Genes for apolipoprotein B and microsomal triglyceride transfer protein are expressed in the heart: evidence that the heart has the capacity to synthesize and secrete lipoproteins." Circulation 98, no. 1 (July 1998): 13-16.

PMID
9665054
Full Text

Gunn, MD, Ngo, VN, Ansel, KM, Ekland, EH, Cyster, JG, and Williams, LT. "A B-cell-homing chemokine made in lymphoid follicles activates Burkitt's lymphoma receptor-1." Nature 391, no. 6669 (February 1998): 799-803.

PMID
9486651
Full Text

Gunn, MD, Tangemann, K, Tam, C, Cyster, JG, Rosen, SD, and Williams, LT. "A chemokine expressed in lymphoid high endothelial venules promotes the adhesion and chemotaxis of naive T lymphocytes." Proceedings of the National Academy of Sciences of the United States of America 95, no. 1 (January 1998): 258-263.

PMID
9419363
Full Text

Nielsen, LB, McCormick, SP, Pierotti, V, Tam, C, Gunn, MD, Shizuya, H, and Young, SG. "Human apolipoprotein B transgenic mice generated with 207- and 145-kilobase pair bacterial artificial chromosomes. Evidence that a distant 5'-element confers appropriate transgene expression in the intestine." J Biol Chem 272, no. 47 (November 21, 1997): 29752-29758.

PMID
9368045
Scholars@Duke

Meiner, V, Tam, C, Gunn, MD, Dong, LM, Weisgraber, KH, Novak, S, Myers, HM, Erickson, SK, and Farese, RV. "Tissue expression studies on the mouse acyl-CoA: cholesterol acyltransferase gene (Acact): findings supporting the existence of multiple cholesterol esterification enzymes in mice." J Lipid Res 38, no. 9 (September 1997): 1928-1933.

PMID
9323603
Scholars@Duke

Gunn, MD, Nelken, NA, Liao, X, and Williams, LT. "Monocyte chemoattractant protein-1 is sufficient for the chemotaxis of monocytes and lymphocytes in transgenic mice but requires an additional stimulus for inflammatory activation." J Immunol 158, no. 1 (January 1, 1997): 376-383.

PMID
8977213
Scholars@Duke

Lauer, MR, Gunn, MD, and Clusin, WT. "Endothelin activates voltage-dependent Ca2+ current by a G protein-dependent mechanism in rabbit cardiac myocytes." J Physiol 448 (March 1992): 729-747.

PMID
1593486
Scholars@Duke

Ueno, H, Gunn, M, Dell, K, Jr, AT, and Williams, L. "A truncated form of fibroblast growth factor receptor 1 inhibits signal transduction by multiple types of fibroblast growth factor receptor." Journal of Biological Chemistry 267, no. 3 (1992): 1470-1476.

PMID
1309784
Scholars@Duke

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