S. Munir Alam, PhD

Professor in Medicine
Professor of Pathology
Member of the Duke Human Vaccine Institute
Campus mail 2 Genome Ct, MSRB II - Room 4004, Durham, NC 27710
Phone (919) 668-6372
Email address alam0004@mc.duke.edu

Research Interests.
Biophysical analysis of coreceptor modulation of TCR-MHC interactions. One of our research interests is to study the molecular mechanisms of T cell recognition. We have particular interest in understanding the trimolecular interactions between membrane bound T cell receptor (TCR-CD3 complex) and its ligand, the peptide-MHC complex (pMHC), and co-receptor molecules. We are using different biophysical approaches which include surface plasmon resonance, isothermal titration calorimetry and atomic force spectroscopy to study the influence of co-stimulatory molecules on the kinetics and thermodynamics of TCR-pMHC binding. The goal is to use integrated molecular and biophysical approaches to study membrane TCR-MHC interactions and to understand how these interactions are modulated by co-receptors during T cell development and activation.
Energetics and kinetics of monoclonal antibody binding to soluble HIV Envelope proteins. An additional interest of our lab involves biophysical characterization of soluble, recombinant human HIV-1 Envelope proteins (gp120 and gp140). We have employed surface plasmon resonance, analytical sedimentation equilibrium analysis, isothermal titration calorimetry and atomic force spectroscopy to characterize the antigenicity and study the energetics, kinetics and single molecule force measurements of the binding of anti-gp120 and anti-gp41 antibodies to several recombinant HIV-1 Envelope proteins. These studies have relevance in immunogen design for generating anti-HIV vaccines.

Education and Training

  • Ph.D., University of Glasgow (Scotland), 1992

Publications

Huang, J, Kang, BH, Pancera, M, Lee, JH, Tong, T, Feng, Y, Imamichi, H, Georgiev, IS, Chuang, G-Y, Druz, A, Doria-Rose, NA, Laub, L, Sliepen, K, van Gils, MJ, de la Peña, AT, Derking, R, Klasse, P-J, Migueles, SA, Bailer, RT, Alam, M, Pugach, P, Haynes, BF, Wyatt, RT, Sanders, RW, Binley, JM, Ward, AB, Mascola, JR, Kwong, PD, and Connors, M. "Broad and potent HIV-1 neutralization by a human antibody that binds the gp41-gp120 interface." Nature 515, no. 7525 (November 2014): 138-142.

PMID
25186731
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Hardy, GJ, Wong, GC, Nayak, R, Anasti, K, Hirtz, M, Shapter, JG, Alam, SM, and Zauscher, S. "HIV-1 antibodies and vaccine antigen selectively interact with lipid domains." Biochimica et biophysica acta 1838, no. 10 (October 2014): 2662-2669.

PMID
25019685
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Kepler, TB, Liao, H-X, Alam, SM, Bhaskarabhatla, R, Zhang, R, Yandava, C, Stewart, S, Anasti, K, Kelsoe, G, Parks, R, Lloyd, KE, Stolarchuk, C, Pritchett, J, Solomon, E, Friberg, E, Morris, L, Karim, SSA, Cohen, MS, Walter, E, Moody, MA, Wu, X, Altae-Tran, HR, Georgiev, IS, Kwong, PD, Boyd, SD, Fire, AZ, Mascola, JR, and Haynes, BF. "Immunoglobulin gene insertions and deletions in the affinity maturation of HIV-1 broadly reactive neutralizing antibodies." Cell host & microbe 16, no. 3 (September 2014): 304-313.

PMID
25211073
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Dennison, SM, Anasti, KM, Jaeger, FH, Stewart, SM, Pollara, J, Liu, P, Kunz, EL, Zhang, R, Vandergrift, N, Permar, S, Ferrari, G, Tomaras, GD, Bonsignori, M, Michael, NL, Kim, JH, Kaewkungwal, J, Nitayaphan, S, Pitisuttithum, P, Rerks-Ngarm, S, Liao, H-X, Haynes, BF, and Alam, SM. "Vaccine-induced HIV-1 envelope gp120 constant region 1-specific antibodies expose a CD4-inducible epitope and block the interaction of HIV-1 gp140 with galactosylceramide." Journal of Virology 88, no. 16 (August 2014): 9406-9417.

PMID
24920809
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Trama, AM, Moody, MA, Alam, SM, Jaeger, FH, Lockwood, B, Parks, R, Lloyd, KE, Stolarchuk, C, Scearce, R, Foulger, A, Marshall, DJ, Whitesides, JF, Jeffries, TL, Wiehe, K, Morris, L, Lambson, B, Soderberg, K, Hwang, K-K, Tomaras, GD, Vandergrift, N, Jackson, KJL, Roskin, KM, Boyd, SD, Kepler, TB, Liao, H-X, and Haynes, BF. "HIV-1 envelope gp41 antibodies can originate from terminal ileum B cells that share cross-reactivity with commensal bacteria." Cell host & microbe 16, no. 2 (August 2014): 215-226.

PMID
25121750
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Gao, F, Bonsignori, M, Liao, HX, Kumar, A, Xia, SM, Lu, X, Cai, F, Hwang, KK, Song, H, Zhou, T, Lynch, RM, Alam, SM, Moody, MA, Ferrari, G, Berrong, M, Kelsoe, G, Shaw, GM, Hahn, BH, Montefiori, DC, Kamanga, G, Cohen, MS, Hraber, P, Kwong, PD, Korber, BT, Mascola, JR, Kepler, TB, and Haynes, BF. "Cooperation of B cell lineages in induction of HIV-1-broadly neutralizing antibodies." Cell 158, no. 3 (July 31, 2014): 481-491.

Full Text

Gao, F, Bonsignori, M, Liao, H-X, Kumar, A, Xia, S-M, Lu, X, Cai, F, Hwang, K-K, Song, H, Zhou, T, Lynch, RM, Alam, SM, Moody, MA, Ferrari, G, Berrong, M, Kelsoe, G, Shaw, GM, Hahn, BH, Montefiori, DC, Kamanga, G, Cohen, MS, Hraber, P, Kwong, PD, Korber, BT, Mascola, JR, Kepler, TB, and Haynes, BF. "Cooperation of B cell lineages in induction of HIV-1-broadly neutralizing antibodies." Cell 158, no. 3 (July 24, 2014): 481-491.

PMID
25065977
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Pollara, J, Bonsignori, M, Moody, MA, Liu, P, Alam, SM, Hwang, K-K, Gurley, TC, Kozink, DM, Armand, LC, Marshall, DJ, Whitesides, JF, Kaewkungwal, J, Nitayaphan, S, Pitisuttithum, P, Rerks-Ngarm, S, Robb, ML, O'Connell, RJ, Kim, JH, Michael, NL, Montefiori, DC, Tomaras, GD, Liao, H-X, Haynes, BF, and Ferrari, G. "HIV-1 vaccine-induced C1 and V2 Env-specific antibodies synergize for increased antiviral activities." Journal of virology 88, no. 14 (July 2014): 7715-7726.

PMID
24807721
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Haynes, BF, Moody, MA, Alam, M, Bonsignori, M, Verkoczy, L, Ferrari, G, Gao, F, Tomaras, GD, Liao, H-X, and Kelsoe, G. "Progress in HIV-1 vaccine development." The Journal of allergy and clinical immunology 134, no. 1 (July 2014): 3-11. (Review)

PMID
25117798
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Osuna, CE, Gonzalez, AM, Chang, H-H, Hung, AS, Ehlinger, E, Anasti, K, Alam, SM, and Letvin, NL. "TCR affinity associated with functional differences between dominant and subdominant SIV epitope-specific CD8+ T cells in Mamu-A*01+ rhesus monkeys." PLoS pathogens 10, no. 4 (April 17, 2014): e1004069-.

PMID
24743648
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