S. Munir Alam, PhD

Professor in Medicine
Professor of Pathology
Member of the Duke Human Vaccine Institute
Campus mail 2 Genome Ct, MSRB II - Room 4004, Durham, NC 27710
Phone (919) 668-6372
Email address alam0004@mc.duke.edu

Research Interests.
Biophysical analysis of coreceptor modulation of TCR-MHC interactions. One of our research interests is to study the molecular mechanisms of T cell recognition. We have particular interest in understanding the trimolecular interactions between membrane bound T cell receptor (TCR-CD3 complex) and its ligand, the peptide-MHC complex (pMHC), and co-receptor molecules. We are using different biophysical approaches which include surface plasmon resonance, isothermal titration calorimetry and atomic force spectroscopy to study the influence of co-stimulatory molecules on the kinetics and thermodynamics of TCR-pMHC binding. The goal is to use integrated molecular and biophysical approaches to study membrane TCR-MHC interactions and to understand how these interactions are modulated by co-receptors during T cell development and activation.
Energetics and kinetics of monoclonal antibody binding to soluble HIV Envelope proteins. An additional interest of our lab involves biophysical characterization of soluble, recombinant human HIV-1 Envelope proteins (gp120 and gp140). We have employed surface plasmon resonance, analytical sedimentation equilibrium analysis, isothermal titration calorimetry and atomic force spectroscopy to characterize the antigenicity and study the energetics, kinetics and single molecule force measurements of the binding of anti-gp120 and anti-gp41 antibodies to several recombinant HIV-1 Envelope proteins. These studies have relevance in immunogen design for generating anti-HIV vaccines.

Education and Training

  • Ph.D., University of Glasgow (Scotland), 1992

Publications

Bonsignori, M, Liao, H-X, Gao, F, Williams, WB, Alam, SM, Montefiori, DC, and Haynes, BF. "Antibody-virus co-evolution in HIV infection: paths for HIV vaccine development." Immunological reviews 275, no. 1 (January 2017): 145-160. (Review)

PMID
28133802
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Bradley, T, Yang, G, Ilkayeva, O, Holl, TM, Zhang, R, Zhang, J, Santra, S, Fox, CB, Reed, SG, Parks, R, Bowman, CM, Bouton-Verville, H, Sutherland, LL, Scearce, RM, Vandergrift, N, Kepler, TB, Moody, MA, Liao, H-X, Alam, SM, McLendon, R, Everitt, JI, Newgard, CB, Verkoczy, L, Kelsoe, G, and Haynes, BF. "HIV-1 Envelope Mimicry of Host Enzyme Kynureninase Does Not Disrupt Tryptophan Metabolism." Journal of immunology (Baltimore, Md. : 1950) 197, no. 12 (December 2016): 4663-4673.

PMID
27849170
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Huang, J, Kang, BH, Ishida, E, Zhou, T, Griesman, T, Sheng, Z, Wu, F, Doria-Rose, NA, Zhang, B, McKee, K, O'Dell, S, Chuang, G-Y, Druz, A, Georgiev, IS, Schramm, CA, Zheng, A, Joyce, MG, Asokan, M, Ransier, A, Darko, S, Migueles, SA, Bailer, RT, Louder, MK, Alam, SM, Parks, R, Kelsoe, G, Von Holle, T, Haynes, BF, Douek, DC, Hirsch, V, Seaman, MS, Shapiro, L, Mascola, JR, Kwong, PD, and Connors, M. "Identification of a CD4-Binding-Site Antibody to HIV that Evolved Near-Pan Neutralization Breadth." Immunity 45, no. 5 (November 2016): 1108-1121.

PMID
27851912
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Bradley, T, Trama, A, Tumba, N, Gray, E, Lu, X, Madani, N, Jahanbakhsh, F, Eaton, A, Xia, S-M, Parks, R, Lloyd, KE, Sutherland, LL, Scearce, RM, Bowman, CM, Barnett, S, Abdool-Karim, SS, Boyd, SD, Melillo, B, Smith, AB, Sodroski, J, Kepler, TB, Alam, SM, Gao, F, Bonsignori, M, Liao, H-X, Moody, MA, Montefiori, D, Santra, S, Morris, L, and Haynes, BF. "Amino Acid Changes in the HIV-1 gp41 Membrane Proximal Region Control Virus Neutralization Sensitivity." EBioMedicine 12 (October 2016): 196-207.

PMID
27612593
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Kahsai, AW, Wisler, JW, Lee, J, Ahn, S, Cahill Iii, TJ, Dennison, SM, Staus, DP, Thomsen, ARB, Anasti, KM, Pani, B, Wingler, LM, Desai, H, Bompiani, KM, Strachan, RT, Qin, X, Alam, SM, Sullenger, BA, and Lefkowitz, RJ. "Conformationally selective RNA aptamers allosterically modulate the β2-adrenoceptor." Nature chemical biology 12, no. 9 (September 2016): 709-716.

PMID
27398998
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Tay, MZ, Liu, P, Williams, LD, McRaven, MD, Sawant, S, Gurley, TC, Xu, TT, Dennison, SM, Liao, H-X, Chenine, A-L, Alam, SM, Moody, MA, Hope, TJ, Haynes, BF, and Tomaras, GD. "Antibody-Mediated Internalization of Infectious HIV-1 Virions Differs among Antibody Isotypes and Subclasses." PLoS pathogens 12, no. 8 (August 31, 2016): e1005817-.

PMID
27579713
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Zhang, R, Alam, SM, Yu, J-S, Scearce, R, Lockwood, B, Hwang, K-K, Parks, R, Permar, S, Brandtzaeg, P, Haynes, BF, and Liao, H-X. "Novel Monoclonal Antibodies for Studies of Human and Rhesus Macaque Secretory Component and Human J-Chain." Monoclonal antibodies in immunodiagnosis and immunotherapy 35, no. 4 (August 2016): 217-226.

PMID
27386924
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Kwon, YD, Georgiev, IS, Ofek, G, Zhang, B, Asokan, M, Bailer, RT, Bao, A, Caruso, W, Chen, X, Choe, M, Druz, A, Ko, S-Y, Louder, MK, McKee, K, O'Dell, S, Pegu, A, Rudicell, RS, Shi, W, Wang, K, Yang, Y, Alger, M, Bender, MF, Carlton, K, Cooper, JW, Blinn, J, Eudailey, J, Lloyd, K, Parks, R, Alam, SM, Haynes, BF, Padte, NN, Yu, J, Ho, DD, Huang, J, Connors, M, Schwartz, RM, Mascola, JR, and Kwong, PD. "Optimization of the Solubility of HIV-1-Neutralizing Antibody 10E8 through Somatic Variation and Structure-Based Design." Journal of virology 90, no. 13 (July 2016): 5899-5914.

PMID
27053554
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Li, H, Wang, S, Kong, R, Ding, W, Lee, F-H, Parker, Z, Kim, E, Learn, GH, Hahn, P, Policicchio, B, Brocca-Cofano, E, Deleage, C, Hao, X, Chuang, G-Y, Gorman, J, Gardner, M, Lewis, MG, Hatziioannou, T, Santra, S, Apetrei, C, Pandrea, I, Alam, SM, Liao, H-X, Shen, X, Tomaras, GD, Farzan, M, Chertova, E, Keele, BF, Estes, JD, Lifson, JD, Doms, RW, Montefiori, DC, Haynes, BF, Sodroski, JG, Kwong, PD, Hahn, BH, and Shaw, GM. "Envelope residue 375 substitutions in simian-human immunodeficiency viruses enhance CD4 binding and replication in rhesus macaques." Proceedings of the National Academy of Sciences of the United States of America 113, no. 24 (June 2016): E3413-E3422.

PMID
27247400
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Bushey, RT, Moody, MA, Nicely, NL, Haynes, BF, Alam, SM, Keir, ST, Bentley, RC, Roy Choudhury, K, Gottlin, EB, Campa, MJ, Liao, H-X, and Patz, EF. "A Therapeutic Antibody for Cancer, Derived from Single Human B Cells." Cell reports 15, no. 7 (May 4, 2016): 1505-1513.

PMID
27160908
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