S. Munir Alam, PhD

Professor in Medicine
Professor of Pathology
Member of the Duke Human Vaccine Institute
Campus mail 2 Genome Ct, MSRB II - Room 4004, Durham, NC 27710
Phone (919) 668-6372
Email address alam0004@mc.duke.edu

Research Interests.
Biophysical analysis of coreceptor modulation of TCR-MHC interactions. One of our research interests is to study the molecular mechanisms of T cell recognition. We have particular interest in understanding the trimolecular interactions between membrane bound T cell receptor (TCR-CD3 complex) and its ligand, the peptide-MHC complex (pMHC), and co-receptor molecules. We are using different biophysical approaches which include surface plasmon resonance, isothermal titration calorimetry and atomic force spectroscopy to study the influence of co-stimulatory molecules on the kinetics and thermodynamics of TCR-pMHC binding. The goal is to use integrated molecular and biophysical approaches to study membrane TCR-MHC interactions and to understand how these interactions are modulated by co-receptors during T cell development and activation.
Energetics and kinetics of monoclonal antibody binding to soluble HIV Envelope proteins. An additional interest of our lab involves biophysical characterization of soluble, recombinant human HIV-1 Envelope proteins (gp120 and gp140). We have employed surface plasmon resonance, analytical sedimentation equilibrium analysis, isothermal titration calorimetry and atomic force spectroscopy to characterize the antigenicity and study the energetics, kinetics and single molecule force measurements of the binding of anti-gp120 and anti-gp41 antibodies to several recombinant HIV-1 Envelope proteins. These studies have relevance in immunogen design for generating anti-HIV vaccines.

Education and Training

  • Ph.D., University of Glasgow (Scotland), 1992

Publications

Zhang, Mei-Yun, Andrew Rosa Borges, Roger G. Ptak, Yanping Wang, Antony S. Dimitrov, S Munir Alam, Lindsay Wieczorek, et al. “Potent and broad neutralizing activity of a single chain antibody fragment against cell-free and cell-associated HIV-1..” Mabs 2, no. 3 (May 2010): 266–74.

PMID
20305395
Scholars@Duke

Moody, M Anthony, Hua-Xin Liao, S Munir Alam, Richard M. Scearce, M Kelly Plonk, Daniel M. Kozink, Mark S. Drinker, et al. “Anti-phospholipid human monoclonal antibodies inhibit CCR5-tropic HIV-1 and induce beta-chemokines..” The Journal of Experimental Medicine 207, no. 4 (April 5, 2010): 763–76. https://doi.org/10.1084/jem.20091281.

PMID
20368576
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Shen, Xiaoying, S Moses Dennison, Pinghuang Liu, Feng Gao, Frederick Jaeger, David C. Montefiori, Laurent Verkoczy, Barton F. Haynes, S Munir Alam, and Georgia D. Tomaras. “Prolonged exposure of the HIV-1 gp41 membrane proximal region with L669S substitution..” Proceedings of the National Academy of Sciences of the United States of America 107, no. 13 (March 15, 2010): 5972–77. https://doi.org/10.1073/pnas.0912381107.

PMID
20231447
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Verkoczy, Laurent, Marilyn Diaz, T Matt Holl, Ying-Bin Ouyang, Hilary Bouton-Verville, S Munir Alam, Hua-Xin Liao, Garnett Kelsoe, and Barton F. Haynes. “Autoreactivity in an HIV-1 broadly reactive neutralizing antibody variable region heavy chain induces immunologic tolerance..” Proc Natl Acad Sci U S A 107, no. 1 (January 5, 2010): 181–86. https://doi.org/10.1073/pnas.0912914107.

PMID
20018688
Full Text

Alam, S Munir, Marco Morelli, S Moses Dennison, Hua-Xin Liao, Ruijun Zhang, Shi-Mao Xia, Sophia Rits-Volloch, et al. “Role of HIV membrane in neutralization by two broadly neutralizing antibodies..” Proc Natl Acad Sci U S A 106, no. 48 (December 1, 2009): 20234–39. https://doi.org/10.1073/pnas.0908713106.

PMID
19906992
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Gao, Feng, Richard M. Scearce, S Munir Alam, Bhavna Hora, Shimao Xia, Julie E. Hohm, Robert J. Parks, et al. “Cross-reactive monoclonal antibodies to multiple HIV-1 subtype and SIVcpz envelope glycoproteins..” Virology 394, no. 1 (November 10, 2009): 91–98. https://doi.org/10.1016/j.virol.2009.07.041.

PMID
19744690
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Shen, X., M. Dennison, F. Gao, D. C. Montefiori, L. Verkoczy, B. Haynes, M. Alam, and G. Tomaras. “OA021-04. HIV-1 gp41 envelope MPER mutation altered epitope conformation in lipid and increased sensitivity to 2F5 and 4E10 neutralizing antibodies.” In Retrovirology, Vol. 6, 2009. https://doi.org/10.1186/1742-4690-6-S3-O16.

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Holl, T. M., M. Kuraoka, D. Liao, L. Verkoczy, M. A. Moody, M. Alam, H. Liao, B. F. Haynes, and G. H. Kelsoe. “P04-44. Generation of antibody responses to HIV-1 membrane proximal external region (MPER) antigen.” Retrovirology 6, no. SUPPL. 3 (October 22, 2009). https://doi.org/10.1186/1742-4690-6-S3-P72.

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Desaire, H., B. F. Haynes, E. P. Go, H. Liao, L. L. Sutherland, Q. Chang, Y. Zhang, J. Irungu, and S. M. Alam. “P20-08. Glycosylation: An important factor in Env diversity.” Retrovirology 6, no. SUPPL. 3 (October 22, 2009). https://doi.org/10.1186/1742-4690-6-S3-P378.

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Verkoczy, L., M. Diaz, T. M. Holl, Y. Ouyang, H. Bouton-Verville, S. M. Alam, H. Liao, G. Kelsoe, and B. F. Haynes. “P04-26. Immunological tolerance prevents the expression of a broadly reactive neutralizing HIV-1 antibody.” Retrovirology 6, no. SUPPL. 3 (October 22, 2009). https://doi.org/10.1186/1742-4690-6-S3-P54.

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