S. Munir Alam, PhD

Professor in Medicine
Professor of Pathology
Member of the Duke Human Vaccine Institute
Campus mail 2 Genome Ct, MSRB II - Room 4004, Durham, NC 27710
Phone (919) 668-6372
Email address alam0004@mc.duke.edu

Research Interests.
Biophysical analysis of coreceptor modulation of TCR-MHC interactions. One of our research interests is to study the molecular mechanisms of T cell recognition. We have particular interest in understanding the trimolecular interactions between membrane bound T cell receptor (TCR-CD3 complex) and its ligand, the peptide-MHC complex (pMHC), and co-receptor molecules. We are using different biophysical approaches which include surface plasmon resonance, isothermal titration calorimetry and atomic force spectroscopy to study the influence of co-stimulatory molecules on the kinetics and thermodynamics of TCR-pMHC binding. The goal is to use integrated molecular and biophysical approaches to study membrane TCR-MHC interactions and to understand how these interactions are modulated by co-receptors during T cell development and activation.
Energetics and kinetics of monoclonal antibody binding to soluble HIV Envelope proteins. An additional interest of our lab involves biophysical characterization of soluble, recombinant human HIV-1 Envelope proteins (gp120 and gp140). We have employed surface plasmon resonance, analytical sedimentation equilibrium analysis, isothermal titration calorimetry and atomic force spectroscopy to characterize the antigenicity and study the energetics, kinetics and single molecule force measurements of the binding of anti-gp120 and anti-gp41 antibodies to several recombinant HIV-1 Envelope proteins. These studies have relevance in immunogen design for generating anti-HIV vaccines.

Education and Training

  • Ph.D., University of Glasgow (Scotland), 1992

Publications

Santra, Sampa, Georgia D. Tomaras, Ranjit Warrier, Nathan I. Nicely, Hua-Xin Liao, Justin Pollara, Pinghuang Liu, et al. “Human Non-neutralizing HIV-1 Envelope Monoclonal Antibodies Limit the Number of Founder Viruses during SHIV Mucosal Infection in Rhesus Macaques..” Plos Pathog 11, no. 8 (August 2015). https://doi.org/10.1371/journal.ppat.1005042.

PMID
26237403
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Permar, Sallie R., Youyi Fong, Nathan Vandergrift, Genevieve G. Fouda, Peter Gilbert, Robert Parks, Frederick H. Jaeger, et al. “Maternal HIV-1 envelope-specific antibody responses and reduced risk of perinatal transmission..” J Clin Invest 125, no. 7 (July 1, 2015): 2702–6. https://doi.org/10.1172/JCI81593.

PMID
26053661
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Nicely, Nathan I., Kevin Wiehe, Thomas B. Kepler, Frederick H. Jaeger, S Moses Dennison, Supachai Rerks-Ngarm, Sorachai Nitayaphan, et al. “Structural analysis of the unmutated ancestor of the HIV-1 envelope V2 region antibody CH58 isolated from an RV144 vaccine efficacy trial vaccinee..” Ebiomedicine 2, no. 7 (July 2015): 713–22. https://doi.org/10.1016/j.ebiom.2015.06.016.

PMID
26288844
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Hulot, Sandrine L., Bette Korber, Elena E. Giorgi, Nathan Vandergrift, Kevin O. Saunders, Harikrishnan Balachandran, Linh V. Mach, et al. “Comparison of Immunogenicity in Rhesus Macaques of Transmitted-Founder, HIV-1 Group M Consensus, and Trivalent Mosaic Envelope Vaccines Formulated as a DNA Prime, NYVAC, and Envelope Protein Boost..” J Virol 89, no. 12 (June 2015): 6462–80. https://doi.org/10.1128/JVI.00383-15.

PMID
25855741
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Fusco, William G., Neelima R. Choudhary, Shelley M. Stewart, S Munir Alam, Gregory D. Sempowski, Christopher Elkins, and Isabelle Leduc. “Defining Potential Vaccine Targets of Haemophilus ducreyi Trimeric Autotransporter Adhesin DsrA..” Monoclon Antib Immunodiagn Immunother 34, no. 2 (April 2015): 73–82. https://doi.org/10.1089/mab.2014.0054.

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25897604
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Liu, Mengfei, Guang Yang, Kevin Wiehe, Nathan I. Nicely, Nathan A. Vandergrift, Wes Rountree, Mattia Bonsignori, et al. “Polyreactivity and autoreactivity among HIV-1 antibodies..” J Virol 89, no. 1 (January 2015): 784–98. https://doi.org/10.1128/JVI.02378-14.

PMID
25355869
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Wiehe, Kevin, David Easterhoff, Kan Luo, Nathan I. Nicely, Todd Bradley, Frederick H. Jaeger, S Moses Dennison, et al. “Antibody light-chain-restricted recognition of the site of immune pressure in the RV144 HIV-1 vaccine trial is phylogenetically conserved..” Immunity 41, no. 6 (December 18, 2014): 909–18. https://doi.org/10.1016/j.immuni.2014.11.014.

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25526306
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Huang, Jinghe, Byong H. Kang, Marie Pancera, Jeong Hyun Lee, Tommy Tong, Yu Feng, Hiromi Imamichi, et al. “Broad and potent HIV-1 neutralization by a human antibody that binds the gp41-gp120 interface..” Nature 515, no. 7525 (November 6, 2014): 138–42. https://doi.org/10.1038/nature13601.

PMID
25186731
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Hardy, Gregory J., Gene C. Wong, Rahul Nayak, Kara Anasti, Michael Hirtz, Joseph G. Shapter, S Munir Alam, and Stefan Zauscher. “HIV-1 antibodies and vaccine antigen selectively interact with lipid domains..” Biochim Biophys Acta 1838, no. 10 (October 2014): 2662–69. https://doi.org/10.1016/j.bbamem.2014.07.007.

PMID
25019685
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Kepler, Thomas B., Hua-Xin Liao, S Munir Alam, Rekha Bhaskarabhatla, Ruijun Zhang, Chandri Yandava, Shelley Stewart, et al. “Immunoglobulin gene insertions and deletions in the affinity maturation of HIV-1 broadly reactive neutralizing antibodies..” Cell Host Microbe 16, no. 3 (September 10, 2014): 304–13. https://doi.org/10.1016/j.chom.2014.08.006.

PMID
25211073
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