S. Munir Alam, PhD

Professor in Medicine
Professor of Pathology
Member of the Duke Human Vaccine Institute
Campus mail 2 Genome Ct, MSRB II - Room 4004, Durham, NC 27710
Phone (919) 668-6372
Email address alam0004@mc.duke.edu

Research Interests.
Biophysical analysis of coreceptor modulation of TCR-MHC interactions. One of our research interests is to study the molecular mechanisms of T cell recognition. We have particular interest in understanding the trimolecular interactions between membrane bound T cell receptor (TCR-CD3 complex) and its ligand, the peptide-MHC complex (pMHC), and co-receptor molecules. We are using different biophysical approaches which include surface plasmon resonance, isothermal titration calorimetry and atomic force spectroscopy to study the influence of co-stimulatory molecules on the kinetics and thermodynamics of TCR-pMHC binding. The goal is to use integrated molecular and biophysical approaches to study membrane TCR-MHC interactions and to understand how these interactions are modulated by co-receptors during T cell development and activation.
Energetics and kinetics of monoclonal antibody binding to soluble HIV Envelope proteins. An additional interest of our lab involves biophysical characterization of soluble, recombinant human HIV-1 Envelope proteins (gp120 and gp140). We have employed surface plasmon resonance, analytical sedimentation equilibrium analysis, isothermal titration calorimetry and atomic force spectroscopy to characterize the antigenicity and study the energetics, kinetics and single molecule force measurements of the binding of anti-gp120 and anti-gp41 antibodies to several recombinant HIV-1 Envelope proteins. These studies have relevance in immunogen design for generating anti-HIV vaccines.

Education and Training

  • Ph.D., University of Glasgow (Scotland), 1992

Publications

Kepler, TB, Munshaw, S, Wiehe, K, Zhang, R, Yu, J-S, Woods, CW, Denny, TN, Tomaras, GD, Alam, SM, Moody, MA, Kelsoe, G, Liao, H-X, and Haynes, BF. "Reconstructing a B-Cell Clonal Lineage. II. Mutation, Selection, and Affinity Maturation." Frontiers in Immunology 5 (January 2014): 170-null.

PMID
24795717
Full Text

Lynch, HE, Stewart, SM, Kepler, TB, Sempowski, GD, and Alam, SM. "Surface plasmon resonance measurements of plasma antibody avidity during primary and secondary responses to anthrax protective antigen." Journal of Immunological Methods 404, no. 1 (2014): 1-12.

Scholars@Duke

Fouda, GG, Jaeger, FH, Amos, JD, Ho, C, Kunz, EL, Anasti, K, Stamper, LW, Liebl, BE, Barbas, KH, Ohashi, T, Moseley, MA, Liao, H-X, Erickson, HP, Alam, SM, and Permar, SR. "Tenascin-C is an innate broad-spectrum, HIV-1-neutralizing protein in breast milk." Proc Natl Acad Sci U S A 110, no. 45 (November 5, 2013): 18220-18225.

PMID
24145401
Full Text

Alam, SM, Dennison, SM, Aussedat, B, Vohra, Y, Park, PK, Fernández-Tejada, A, Stewart, S, Jaeger, FH, Anasti, K, Blinn, JH, Kepler, TB, Bonsignori, M, Liao, H-X, Sodroski, JG, Danishefsky, SJ, and Haynes, BF. "Recognition of synthetic glycopeptides by HIV-1 broadly neutralizing antibodies and their unmutated ancestors." Proc Natl Acad Sci U S A 110, no. 45 (November 5, 2013): 18214-18219.

PMID
24145434
Full Text

Gao, F, Bonsignori, M, Liao, H, Kumar, A, Xia, S, Cai, F, Lu, X, Kozink, DM, Kwong, P, Zhou, T, Lynch, R, Alam, SM, Ferrari, G, Kelsoe, G, Sandrasegaram, G, Shaw, GM, Hahn, BH, Montefiori, DC, Kamanga, G, Cohen, M, Braber, P, Korber, BT, Mascola, JR, Kepler, TB, and Haynes, BF. "Multiple Pathways of HIV-1 Autologous Neutralizing Antibodies Cooperate to Drive CD4 Binding Site Broadly Neutralizing Antibody Responses." November 1, 2013.

Scholars@Duke

Chen, Y, Zhang, J, Bouton-Verville, H, Newman, A, Xia, S, Liao, H, Montefiori, DC, Moody, MA, Armand, L, Hwang, K, Lockwood, B, Dennison, SM, Alam, SM, Haynes, BF, and Verkoczy, L. "Lipid Components in MPER-Based Immunization Regimens Are Critical for Inducing Broadly Neutralizing Antibody Responses in 2F5 and 4E10 Knockin Mice." November 1, 2013.

Scholars@Duke

Nicely, NI, Wiehe, K, Kepler, TB, Jaeger, FH, Dennison, SM, Liao, H, Alam, SM, Rerks-Ngarm, S, Nitayaphan, S, Pitisuttithum, P, Kaewkungwal, J, Michael, NL, Kim, JH, and Haynes, BF. "Structural Analysis of the Unmutated Common Ancestor Antibodies of the HIV Envelope V2 Antibodies CH58 and CH59 Derived from RV144 Vaccinees." November 1, 2013.

Scholars@Duke

Trama, AM, Liao, H, Jaeger, FH, Alam, SM, Wiehe, K, Jr, JTL, Foulger, A, Williams, WT, Marshall, DJ, Stolarchuk, C, Lloyd, KE, Parks, R, Soderberg, K, Kepler, TB, Vandergrift, N, Jackson, KJ, Fire, AZ, Boyd, SD, Whitesides, JF, Tomaras, GD, Moody, MA, and Haynes, BF. "Intestinal Commensal Bacteria Shape the Pre-transmission Antibody Repertoire to HIV-1 Infection." November 1, 2013.

Scholars@Duke

Aussedat, B, Vohra, Y, Park, PK, Fernández-Tejada, A, Alam, SM, Dennison, SM, Jaeger, FH, Anasti, K, Stewart, S, Blinn, JH, Liao, H-X, Sodroski, JG, Haynes, BF, and Danishefsky, SJ. "Chemical synthesis of highly congested gp120 V1V2 N-glycopeptide antigens for potential HIV-1-directed vaccines." J Am Chem Soc 135, no. 35 (September 4, 2013): 13113-13120.

PMID
23915436
Full Text

Verkoczy, L, Chen, Y, Zhang, J, Bouton-Verville, H, Newman, A, Lockwood, B, Scearce, RM, Montefiori, DC, Dennison, SM, Xia, S-M, Hwang, K-K, Liao, H-X, Alam, SM, and Haynes, BF. "Induction of HIV-1 broad neutralizing antibodies in 2F5 knock-in mice: selection against membrane proximal external region-associated autoreactivity limits T-dependent responses." J Immunol 191, no. 5 (September 1, 2013): 2538-2550.

PMID
23918977
Full Text

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