Duke University Pulmonary Research Laboratories

The core pulmonary laboratory in the Medical Sciences Research Building occupies 10,000 square feet and has been designed and equipped for chemical, biochemical, cell and molecular biology, and physiological studies.

The facilities include a walk-in cold room, cell culture room, equipment room, and biochemistry bioassay preparations. Animal support facilities are provided by the Duke University Vivarium which is fully accredited by the American Association for the Accreditation of Laboratory Animal Care.

Computer resources include a network of Macintosh and IBM PC's for data acquisition (cellular, bio-chemical, and in vivo models), storage, and statistical analysis.

Major equipment includes:

  • UV/Vis and fluorescent spectrophotometer
  • Luminometer
  • HPLC (UV/Vis fluorescent and electrochemical detectors)
  • Capillary electrophoresis
  • Photolysis/chemi-luminescence
  • FPLC
  • GC mass spectrometer with electrospray ionization capabilities
  • Fast gel systems
  • Coulter counter
  • Micro-plate reader
  • Fully equipped and automated darkroom including a liford Rapid Print Processor
  • Multiple thermocyclers
  • Tonometry apparatus ultracentrifuges
  • Gamma and beta liquid scintillation counters
  • Intact bioassay systems for smooth muscle
  • GT03 force transducer
  • Grass polygraph
  • Grass ECG cables and amplifier
  • Fiber-Lite Spectramed pressure transducer (Model P23XL)
  • DATAQ instruments computerized programs for data collection and analysis
  • ABI DNA sequencer
  • Required minor laboratory equipment


Visit each Lab below.

The Ingram Lab’s research interests focus on the study of airway remodeling in human asthma.

  • Patty Lee Lab

The Lee Lab’s research interests focus on acute and chronic oxidant-induced lung injury and repair, specifically the distinct roles of stress-response pathways.

  • Harvey Marshall Lab

The Marshall Lab’s research interests focus on acute lung injury.

  • Timothy McMahon Lab

The McMahon Lab at Duke University and Durham VA Medical Center is investigating novel roles of the red blood cell (RBC) in the circulation.

  • David Murdoch Lab

 Dr. Murdoch’s research focuses on T cell immunology utilizing a variety of platforms including polychromatic flow cytometry, cytokine multiplexing, and novel single cell assays.

The Palmer Lab conducts basic and clinical research on lung transplantation and its complications..

  • Loretta Que Lab

The Que Lab’s research interests focus on studying the role of nitric oxide and related enzymes in the pathogenesis of lung disease, specifically that caused by nitrosative/oxidative stress. 

  • Robert Tighe Lab

The Tighe Lab’s research focus is to identify susceptibility factors and candidate pathways relevant to host biological responses to environmental pollutants.