Duke University Pulmonary Research Laboratories

The core pulmonary laboratory in the Medical Sciences Research Building occupies 10,000 square feet and has been designed and equipped for chemical, biochemical, cell and molecular biology, and physiological studies.

The facilities include a walk-in cold room, cell culture room, equipment room, and biochemistry bioassay preparations. Animal support facilities are provided by the Duke University Vivarium which is fully accredited by the American Association for the Accreditation of Laboratory Animal Care.

Computer resources include a network of Macintosh and IBM PC's for data acquisition (cellular, bio-chemical, and in vivo models), storage, and statistical analysis.

Major equipment includes:

  • UV/Vis and fluorescent spectrophotometer
  • Luminometer
  • HPLC (UV/Vis fluorescent and electrochemical detectors)
  • Capillary electrophoresis
  • Photolysis/chemi-luminescence
  • FPLC
  • GC mass spectrometer with electrospray ionization capabilities
  • Fast gel systems
  • Coulter counter
  • Micro-plate reader
  • Fully equipped and automated darkroom including a liford Rapid Print Processor
  • Multiple thermocyclers
  • Tonometry apparatus ultracentrifuges
  • Gamma and beta liquid scintillation counters
  • Intact bioassay systems for smooth muscle
  • GT03 force transducer
  • Grass polygraph
  • Grass ECG cables and amplifier
  • Fiber-Lite Spectramed pressure transducer (Model P23XL)
  • DATAQ instruments computerized programs for data collection and analysis
  • ABI DNA sequencer
  • Required minor laboratory equipment