David Stephen Pisetsky, MD, PhD

Professor of Medicine
Professor of Immunology
Member of the Duke Cancer Institute
Member of the Duke Human Vaccine Institute
Campus mail VA Med Ctr, 508 Fulton St, Durham, NC 27705
Phone (919) 286-6835
Email address david.pisetsky@duke.edu

Studies in my laboratory concern the immunological properties of DNA as related to two main topics: 1) the induction of anti-DNA responses in systemic lupus erythematosus; and 2) the stimulation of innate immunity by bacterial DNA. These topics are closely linked since we have established novel disease models in which lupus-like illness can be induced in normal mice by bacterial DNA under conditions in which mammalian DNA is inactive. This model has been useful in elucidating mechanisms of DNA antigen drive in autoimmunity.

To pursue these studies, our laboratory conducts investigations in the following areas: 1) specificity of anti-DNA for epitopes on mammalian and bacterial DNA; 2) molecular analysis of murine mononclonal anti-DNA antibodies; 3) histopathological analyses of DNA-immunized mice; 4) in vitro analysis of proliferation, antibody production and cytokine expression in human and murine immune cells; and 5) analysis of DNA binding to cell surface molecules on B cells, T cells and macrophages. Results of these studies have allowed identification of at least two structural motifs that are immunoactive. We are also exploring the impact of chemical modifications of the DNA backbone.

In addition to work on the immunology of DNA, I am also involved in clinical trials related to new immunomodulatory agents in the treatment of rheumatoid arthritis as well as serological markers of disease activity.

The areas of research for which I am known nationally are anti-DNA antibodies, systemic lupus erythematosus and immunological properties of DNA. I have written textbook chapters and reviews on all these subjects.

Education and Training

  • Resident, Medicine, Yale University School of Medicine, 1974 - 1974
  • Intern, Medicine, Yale University School of Medicine, 1973 - 1974
  • M.D., Yeshiva University, Albert Einstein College of Medicine, 1973
  • Ph.D., Yeshiva University, Albert Einstein College of Medicine, 1972


Naqvi, Ibtehaj, Ruwan Gunaratne, Jessica E. McDade, Angelo Moreno, Rachel E. Rempel, Douglas C. Rouse, Silvia Gabriela Herrera, et al. “Polymer-Mediated Inhibition of Pro-invasive Nucleic Acid DAMPs and Microvesicles Limits Pancreatic Cancer Metastasis.” Mol Ther 26, no. 4 (April 4, 2018): 1020–31. https://doi.org/10.1016/j.ymthe.2018.02.018.

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Spencer, Diane M., John R. Dye, Claude A. Piantadosi, and David S. Pisetsky. “The release of microparticles and mitochondria from RAW 264.7 murine macrophage cells undergoing necroptotic cell death in vitro.” Exp Cell Res 363, no. 2 (February 15, 2018): 151–59. https://doi.org/10.1016/j.yexcr.2017.12.024.

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Frank-Bertoncelj, Mojca, David S. Pisetsky, Christoph Kolling, Beat A. Michel, Renate E. Gay, Astrid Jüngel, and Steffen Gay. “TLR3 Ligand Poly(I:C) Exerts Distinct Actions in Synovial Fibroblasts When Delivered by Extracellular Vesicles.” Front Immunol 9 (2018): 28. https://doi.org/10.3389/fimmu.2018.00028.

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Théry, Clotilde, Kenneth W. Witwer, Elena Aikawa, Maria Jose Alcaraz, Johnathon D. Anderson, Ramaroson Andriantsitohaina, Anna Antoniou, et al. “Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines.” J Extracell Vesicles 7, no. 1 (2018): 1535750. https://doi.org/10.1080/20013078.2018.1535750.

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Zerwas, Stephanie, Janne Tidselbak Larsen, Liselotte Petersen, Laura M. Thornton, Michela Quaranta, Susanne Vinkel Koch, David Pisetsky, Preben Bo Mortensen, and Cynthia M. Bulik. “Eating Disorders, Autoimmune, and Autoinflammatory Disease.” Pediatrics 140, no. 6 (December 2017). https://doi.org/10.1542/peds.2016-2089.

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Borenstein, David G., Afton L. Hassett, and David Pisetsky. “Pain management in rheumatology research, training, and practice.” Clin Exp Rheumatol 35 Suppl 107, no. 5 (September 2017): 2–7.