David Stephen Pisetsky, MD, PhD

Professor of Medicine
Professor of Immunology
Member of the Duke Cancer Institute
Member of the Duke Human Vaccine Institute
Campus mail VA Med Ctr, 508 Fulton St, Durham, NC 27705
Phone (919) 286-6835
Email address david.pisetsky@duke.edu

Studies in my laboratory concern the immunological properties of DNA as related to two main topics: 1) the induction of anti-DNA responses in systemic lupus erythematosus; and 2) the stimulation of innate immunity by bacterial DNA. These topics are closely linked since we have established novel disease models in which lupus-like illness can be induced in normal mice by bacterial DNA under conditions in which mammalian DNA is inactive. This model has been useful in elucidating mechanisms of DNA antigen drive in autoimmunity.

To pursue these studies, our laboratory conducts investigations in the following areas: 1) specificity of anti-DNA for epitopes on mammalian and bacterial DNA; 2) molecular analysis of murine mononclonal anti-DNA antibodies; 3) histopathological analyses of DNA-immunized mice; 4) in vitro analysis of proliferation, antibody production and cytokine expression in human and murine immune cells; and 5) analysis of DNA binding to cell surface molecules on B cells, T cells and macrophages. Results of these studies have allowed identification of at least two structural motifs that are immunoactive. We are also exploring the impact of chemical modifications of the DNA backbone.

In addition to work on the immunology of DNA, I am also involved in clinical trials related to new immunomodulatory agents in the treatment of rheumatoid arthritis as well as serological markers of disease activity.

The areas of research for which I am known nationally are anti-DNA antibodies, systemic lupus erythematosus and immunological properties of DNA. I have written textbook chapters and reviews on all these subjects.

Education and Training

  • Resident, Medicine, Yale University School of Medicine, 1974 - 1974
  • Intern, Medicine, Yale University School of Medicine, 1973 - 1974
  • M.D., Yeshiva University, Albert Einstein College of Medicine, 1973
  • Ph.D., Yeshiva University, Albert Einstein College of Medicine, 1972

Publications

Leuchten, Nicolai, Bettina Bauernfeind, Johannes Kuttner, Tanja Stamm, Josef S. Smolen, David S. Pisetsky, and Martin Aringer. “Relevant concepts of functioning for patients with systemic lupus erythematosus identified in a Delphi exercise of experts and a literature review.” Arthritis Care Res (Hoboken) 66, no. 12 (December 2014): 1895–1904. https://doi.org/10.1002/acr.22372.

PMID
24839085
Full Text

Grossman, Jennifer M., Megan E. B. Clowse, Peter M. Izmirly, Diane L. Kamen, Alana K. Levine, Meggan Mackey, W Joseph McCune, et al. “Hydroxychloroquine Dosing and Disease Activity in a Large Multi-Racial Lupus Cohort.” Arthritis & Rheumatology 66 (October 1, 2014): S296–97.

Scholars@Duke

Pisetsky, David S., Diane Spencer, Stephen R. Wisniewski, Jason Lyons, Melissa Saul, Mandy J. McGeachy, Yong Gil Hwang, Heather Eng, and Larry W. Moreland. “The Relationship Between Disease Activity and Levels of HMGB1 in Patients with Rheumatoid Arthritis.” In Arthritis & Rheumatology, 66:S172–S172. WILEY-BLACKWELL, 2014.

Scholars@Duke

Ullal, Anirudh J., Tony N. Marion, and David S. Pisetsky. “The role of antigen specificity in the binding of murine monoclonal anti-DNA antibodies to microparticles from apoptotic cells.” Clin Immunol 154, no. 2 (October 2014): 178–87. https://doi.org/10.1016/j.clim.2014.05.007.

PMID
24873886
Full Text

Pisetsky, D. S., S. E. Trace, K. A. Brownley, R. M. Hamer, N. L. Zucker, P. Roux-Lombard, J. -. M. Dayer, and C. M. Bulik. “The expression of cytokines and chemokines in the blood of patients with severe weight loss from anorexia nervosa: an exploratory study.” Cytokine 69, no. 1 (September 2014): 110–15. https://doi.org/10.1016/j.cyto.2014.05.018.

PMID
25022969
Full Text

Spencer, D. M., F. Mobarrez, H. Wallén, and D. S. Pisetsky. “The expression of HMGB1 on microparticles from Jurkat and HL-60 cells undergoing apoptosis in vitro.” Scand J Immunol 80, no. 2 (August 2014): 101–10. https://doi.org/10.1111/sji.12191.

PMID
24846056
Full Text

Wang, Xiao, Nancy A. Stearns, Xingfu Li, and David S. Pisetsky. “The effect of polyamines on the binding of anti-DNA antibodies from patients with SLE and normal human subjects.” Clin Immunol 153, no. 1 (July 2014): 94–103. https://doi.org/10.1016/j.clim.2014.04.003.

PMID
24732074
Full Text

Spencer, Diane M., Julie Gauley, and David S. Pisetsky. “The properties of microparticles from RAW 264.7 macrophage cells undergoing in vitro activation or apoptosis.” Innate Immun 20, no. 3 (April 2014): 239–48. https://doi.org/10.1177/1753425913492552.

PMID
23839527
Full Text

Pages